##################################
# NO CUT ENZYMES ( 33 enzymes) #
##################################
AgeI Alw44I ApaLI AscI BciVI
BsiWI BsmBI BspDI BssHII Bst1107I
BstZ17I ClaI CpoI EcoRV FseI
HpaI KspI MfeI MluI MunI
NotI NruI PacI PinAI PmeI
PvuI RsrII SacII SalI SgrAI
SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 2105 CGCG CG^CG
AflIII 4567 ACATGT A^C[GA][CT]GT
AhdI 2298 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 291 ATTAAT AT^TAAT
AsnI 291 ATTAAT AT^TAAT
AspEI 2298 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1485 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3721 TGCGCA TGC^GCA
BbrPI 1038 CACGTG CAC^GTG
BsaBI 381 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 4567 ACATGT A^CATGT
BsrBI 5661 CCGCTC CCGCTC(-3/-3)
BstUI 2105 CGCG CG^CG
DrdI 5256 GACGTCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2298 GACATGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 801 GAATTC G^AATTC
EcoT22I 304 ATGCAT ATGCA^T
FspI 3721 TGCGCA TGC^GCA
HincII 735 GTTGAC GT[CT]^[GA]AC
HindII 735 GTTGAC GT[CT]^[GA]AC
HindIII 1842 AAGCTT A^AGCTT
MamI 381 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2105 CGCG CG^CG
NheI 2897 GCTAGC G^CTAGC
NsiI 304 ATGCAT ATGCA^T
PflFI 1485 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1038 CACGTG CAC^GTG
PmlI 1038 CACGTG CAC^GTG
PshAI 7228 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 291 ATTAAT AT^TAAT
ScaI 4606 AGTACT AGT^ACT
SspI 48 AATATT AAT^ATT
Tth111I 1485 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 6202 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 3797 GACGTC GACGT^C
5256 GACGTC
AccIII 4471 TCCGGA T^CCGGA
6043 TCCGGA
AflII 5700 CTTAAG C^TTAAG
5745 CTTAAG
Aor51HI 2030 AGCGCT AGC^GCT
3839 AGCGCT
AvrII 6831 CCTAGG C^CTAGG
7328 CCTAGG
BamHI 1912 GGATCC G^GATCC
4711 GGATCC
BbeI 2212 GGCGCC GGCGC^C
3927 GGCGCC
BclI 1306 TGATCA T^GATCA
2333 TGATCA
BfrI 5700 CTTAAG C^TTAAG
5745 CTTAAG
BglII 523 AGATCT A^GATCT
4854 AGATCT
BlnI 6831 CCTAGG C^CTAGG
7328 CCTAGG
BsaAI 1038 CACGTG [CT]AC^GT[GA]
3753 TACGTG
BseAI 4471 TCCGGA T^CCGGA
6043 TCCGGA
BspEI 4471 TCCGGA T^CCGGA
6043 TCCGGA
BstBI 4483 TTCGAA TT^CGAA
6654 TTCGAA
BstXI 2516 CCAGAACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6607 CCAGCACTTTGG
EagI 4033 CGGCCG C^GGCCG
6018 CGGCCG
EclXI 4033 CGGCCG C^GGCCG
6018 CGGCCG
Eco47III 2030 AGCGCT AGC^GCT
3839 AGCGCT
Eco52I 4033 CGGCCG C^GGCCG
6018 CGGCCG
FbaI 1306 TGATCA T^GATCA
2333 TGATCA
KasI 2212 GGCGCC G^GCGCC
3927 GGCGCC
MroI 4471 TCCGGA T^CCGGA
6043 TCCGGA
NarI 2212 GGCGCC GG^CGCC
3927 GGCGCC
NdeI 1010 CATATG CA^TATG
1834 CATATG
NspV 4483 TTCGAA TT^CGAA
6654 TTCGAA
PaeR7I 6183 CTCGAG C^TCGAG
6458 CTCGAG
SbfI 2648 CCTGCAGG CCTGCA^GG
3083 CCTGCAGG
SexAI 2728 ACCTGGT A^CC[AT]GGT
5068 ACCTGGT
SfiI 2267 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5409 GGCCCACGGGGCC
SfoI 2212 GGCGCC GGC^GCC
3927 GGCGCC
SfuI 4483 TTCGAA TT^CGAA
6654 TTCGAA
Sse8387I 2648 CCTGCAGG CCTGCA^GG
3083 CCTGCAGG
XhoI 6183 CTCGAG C^TCGAG
6458 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 157 enzymes) #
#########################################
Acc65I AccI AciI AclI AcsI
AcyI AfaI AluI AlwI AlwNI
AocI ApaI ApoI Asp700I Asp718I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
Psp1406I PstI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI