##################################
# NO CUT ENZYMES (   65 enzymes) #
##################################

AatII       AclI        AgeI        Aor51HI     ApaI        
AscI        AviII       AvrII       BbeI        BbrPI       
BcgI        BglI        BlnI        BlpI        Bpu1102I    
BsiEI       BsiWI       BspDI       BspHI       BsrFI       
BssHII      Bst1107I    BstZ17I     CelII       Cfr10I      
ClaI        CpoI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       FseI        FspI        KasI        
KspI        MluI        NaeI        NarI        NgoMIV      
NotI        NruI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
Sse8387I    XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       1838  GGTACC               G^GTACC
AccI         7086  GTAGAC               GT^[AC][GT]AC
AccIII        812  TCCGGA               T^CCGGA
AcyI          340  GGCGTC               G[GA]^CG[CT]C
AhdI         6501  GACCTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1252  GTGCAC               G^TGCAC
ApaLI        1252  GTGCAC               G^TGCAC
Asp718I      1838  GGTACC               G^GTACC
AspEI        6501  GACCTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         409  GGATCC               G^GATCC
BbvCI        1763  GCTGAGG              CCTCAGC(-5/-2)
BglII        4145  AGATCT               A^GATCT
BsaHI         340  GGCGTC               G[GA]^CG[CT]C
BseAI         812  TCCGGA               T^CCGGA
BsgI         2530  CTGCAC               GTGCAG(16/14)
BsmBI        3535  GAGACG               CGTCTC(1/5)
BspEI         812  TCCGGA               T^CCGGA
BsrBI         808  CCGCTC               CCGCTC(-3/-3)
BstBI        3505  TTCGAA               TT^CGAA
DraIII       2859  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          325  GACTCCGTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     6501  GACCTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2679  GAATTC               G^AATTC
HaeII         102  GGCGCT               [GA]GCGC^[CT]
Hin1I         340  GGCGTC               G[GA]^CG[CT]C
HincII        782  GTTAAC               GT[CT]^[GA]AC
HindII        782  GTTAAC               GT[CT]^[GA]AC
HpaI          782  GTTAAC               GTT^AAC
KpnI         1838  GGTACC               GGTAC^C
MfeI         4600  CAATTG               C^AATTG
MroI          812  TCCGGA               T^CCGGA
MunI         4600  CAATTG               C^AATTG
NheI         6943  GCTAGC               G^CTAGC
NspV         3505  TTCGAA               TT^CGAA
PshAI        2611  GACGACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          953  GAGCTC               GAGCT^C
SfuI         3505  TTCGAA               TT^CGAA
SnaBI        4024  TACGTA               TAC^GTA
StuI         6715  AGGCCT               AGG^CCT
SwaI         5756  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AflII         970  CTTAAG               C^TTAAG
             2631  CTTAAG              
AspI          176  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              325  GACTCCGTC           
AvaI          569  CTCGGG               C^[CT]CG[GA]G
              933  CTCGGG              
BalI         4083  TGGCCA               TGG^CCA
             6847  TGGCCA              
BfrI          970  CTTAAG               C^TTAAG
             2631  CTTAAG              
BsaAI        3993  CACGTA               [CT]AC^GT[GA]
             4024  TACGTA              
BsaI          378  GAGACC               GGTCTC(1/5)
             6916  GGTCTC              
BsoBI         569  CTCGGG               C^[CT]CG[GA]G
              933  CTCGGG              
Bsp1407I     5096  TGTACA               T^GTACA
             5594  TGTACA              
BspMI         549  ACCTGC               ACCTGC(4/8)
             2534  ACCTGC              
BsrDI        2136  GCAATG               GCAATG(2/0)
             5995  GCAATG              
BsrGI        5096  TGTACA               T^GTACA
             5594  TGTACA              
BssSI         243  CACGAG               CACGAG(-5/-1)
              488  CACGAG              
DraII        1404  AGGACCT              [GA]G^G[ACGT]CC[CT]
             6854  AGGTCCT             
EcoO109I     1404  AGGACCT              [GA]G^G[ACGT]CC[CT]
             6854  AGGTCCT             
EcoT22I      6391  ATGCAT               ATGCA^T
             6831  ATGCAT              
HindIII       698  AAGCTT               A^AGCTT
             2029  AAGCTT              
MluNI        4083  TGGCCA               TGG^CCA
             6847  TGGCCA              
MscI         4083  TGGCCA               TGG^CCA
             6847  TGGCCA              
NsiI         6391  ATGCAT               ATGCA^T
             6831  ATGCAT              
PflFI         176  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              325  GACTCCGTC           
PflMI        1842  CCAGACTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6306  CCACATCTTGG         
PpuMI        1404  AGGACCT              [GA]G^G[AT]CC[CT]
             6854  AGGTCCT             
SapI          129  GAAGAGC              GCTCTTC(1/4)
              955  GCTCTTC             
SpeI         4339  ACTAGT               A^CTAGT
             6641  ACTAGT              
SphI          922  GCATGC               GCATG^C
              964  GCATGC              
SspBI        5096  TGTACA               T^GTACA
             5594  TGTACA              
Tth111I       176  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              325  GACTCCGTC           
Van91I       1842  CCAGACTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             6306  CCACATCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
AseI        AsnI        Asp700I     AspHI       AvaII       
BanI        BanII       BbsI        BbvI        BciVI       
BclI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaBI       BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspLU11I    BsrI        BssKI       
BstAPI      BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Bsu36I      Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DsaI        EaeI        EarI        Eco81I      EcoO65I     
EcoRII      EcoRV       EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PshBI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII       XmnI