##################################
# NO CUT ENZYMES ( 37 enzymes) #
##################################
Acc65I AgeI AscI Asp718I AvrII
BbeI BcgI BlnI BsaBI BsiWI
BssHII BstBI CpoI EcoRI EcoRV
HpaI KasI KpnI MamI MfeI
MluI MunI NarI NotI NruI
NspV PacI PinAI PmeI PshAI
RsrII SalI SfoI SfuI SgrAI
SnaBI SpeI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 463 GACGTC GACGT^C
AhdI 2656 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2656 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 6210 TGCGCA TGC^GCA
BciVI 3598 GGATAC GTATCC(6/5)
BglII 7178 AGATCT A^GATCT
Bsp1407I 7190 TGTACA T^GTACA
BspDI 919 ATCGAT AT^CGAT
BspHI 2708 TCATGA T^CATGA
BsrGI 7190 TGTACA T^GTACA
Bst1107I 7645 GTATAC GTA^TAC
BstEII 2844 GGTGACC G^GT[ACGT]ACC
BstPI 2844 GGTGACC G^GT[ACGT]ACC
BstZ17I 7645 GTATAC GTA^TAC
ClaI 919 ATCGAT AT^CGAT
EagI 2161 CGGCCG C^GGCCG
Eam1105I 2656 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2161 CGGCCG C^GGCCG
Eco52I 2161 CGGCCG C^GGCCG
EcoO65I 2844 GGTGACC G^GT[ACGT]ACC
FseI 2158 GGCCGGCC GGCCGG^CC
FspI 6210 TGCGCA TGC^GCA
HincII 2992 GTCAAC GT[CT]^[GA]AC
HindII 2992 GTCAAC GT[CT]^[GA]AC
KspI 5244 CCGCGG CCGC^GG
NheI 1332 GCTAGC G^CTAGC
PaeR7I 2737 CTCGAG C^TCGAG
PvuI 3118 CGATCG CGAT^CG
RcaI 2708 TCATGA T^CATGA
SacII 5244 CCGCGG CCGC^GG
ScaI 6442 AGTACT AGT^ACT
SexAI 1913 ACCTGGT A^CC[AT]GGT
SfiI 1028 GGCCACTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 4855 GCATGC GCATG^C
SspBI 7190 TGTACA T^GTACA
SwaI 5639 ATTTAAAT ATTT^AAAT
XbaI 3694 TCTAGA T^CTAGA
XhoI 2737 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 4932 GTCTAC GT^[AC][GT]AC
7645 GTATAC
AccIII 824 TCCGGA T^CCGGA
1931 TCCGGA
AclI 2143 AACGTT AA^CGTT
7424 AACGTT
AflII 3409 CTTAAG C^TTAAG
3830 CTTAAG
Alw44I 1369 GTGCAC G^TGCAC
3648 GTGCAC
ApaLI 1369 GTGCAC G^TGCAC
3648 GTGCAC
AseI 5745 ATTAAT AT^TAAT
6921 ATTAAT
AsnI 5745 ATTAAT AT^TAAT
6921 ATTAAT
Asp700I 5455 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6419 GAAGAGCTTC
BbrPI 1690 CACGTG CAC^GTG
2113 CACGTG
BfrI 3409 CTTAAG C^TTAAG
3830 CTTAAG
BsaWI 824 TCCGGA [AT]^CCGG[AT]
1931 TCCGGA
BseAI 824 TCCGGA T^CCGGA
1931 TCCGGA
BsmBI 465 CGTCTC CGTCTC(1/5)
2889 CGTCTC
BspEI 824 TCCGGA T^CCGGA
1931 TCCGGA
BspLU11I 5396 ACATGT A^CATGT
7463 ACATGT
BsrDI 1560 CATTGC GCAATG(2/0)
5990 GCAATG
BssSI 691 CACGAG CACGAG(-5/-1)
1477 CTCGTG
DrdI 780 GACGCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2053 GACCCCATTGTC
EcoT22I 3616 ATGCAT ATGCA^T
5371 ATGCAT
HindIII 3917 AAGCTT A^AGCTT
7639 AAGCTT
MroI 824 TCCGGA T^CCGGA
1931 TCCGGA
NdeI 5277 CATATG CA^TATG
6247 CATATG
NsiI 3616 ATGCAT ATGCA^T
5371 ATGCAT
PmaCI 1690 CACGTG CAC^GTG
2113 CACGTG
PmlI 1690 CACGTG CAC^GTG
2113 CACGTG
PshBI 5745 ATTAAT AT^TAAT
6921 ATTAAT
Psp1406I 2143 AACGTT AA^CGTT
7424 AACGTT
SacI 1320 GAGCTC GAGCT^C
7242 GAGCTC
SapI 6419 GAAGAGC GCTCTTC(1/4)
4561 GCTCTTC
SbfI 2187 CCTGCAGG CCTGCA^GG
2985 CCTGCAGG
SmaI 2444 CCCGGG CCC^GGG
7253 CCCGGG
Sse8387I 2187 CCTGCAGG CCTGCA^GG
2985 CCTGCAGG
XmaCI 2444 CCCGGG C^CCGGG
7253 CCCGGG
XmaI 2444 CCCGGG C^CCGGG
7253 CCCGGG
XmnI 5455 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6419 GAAGAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 147 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI ApoI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII