##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
AhdI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       BamHI       BciVI       BclI        
BfrI        BglII       BsaBI       BsiWI       Bsp1407I    
BspDI       BspLU11I    BsrGI       BssSI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraIII      
DrdI        Eam1105I    EcoRI       EcoRV       FbaI        
HincII      HindII      HindIII     HpaI        KpnI        
MamI        MfeI        MluI        MunI        NheI        
NotI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SalI        ScaI        SfiI        SfuI        
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII         955  GACGTC               GACGT^C
AccIII       1033  TCCGGA               T^CCGGA
Alw44I       3961  GTGCAC               G^TGCAC
ApaLI        3961  GTGCAC               G^TGCAC
AspI         1397  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3155  CCTAGG               C^CTAGG
BbvCI        1373  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1573  GCAGGAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         3155  CCTAGG               C^CTAGG
BseAI        1033  TCCGGA               T^CCGGA
BspEI        1033  TCCGGA               T^CCGGA
BspHI        2667  TCATGA               T^CATGA
BsrDI         371  GCAATG               GCAATG(2/0)
BstEII       2500  GGTCACC              G^GT[ACGT]ACC
BstPI        2500  GGTCACC              G^GT[ACGT]ACC
DraI          856  TTTAAA               TTT^AAA
EcoO65I      2500  GGTCACC              G^GT[ACGT]ACC
EcoT22I      1040  ATGCAT               ATGCA^T
FseI          267  GGCCGGCC             GGCCGG^CC
MroI         1033  TCCGGA               T^CCGGA
NdeI          574  CATATG               CA^TATG
NruI         1582  TCGCGA               TCG^CGA
NsiI         1040  ATGCAT               ATGCA^T
NspI         1791  ACATGC               [GA]CATG^[CT]
PflFI        1397  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2494  GACCCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2667  TCATGA               T^CATGA
SacI         3331  GAGCTC               GAGCT^C
SbfI         2317  CCTGCAGG             CCTGCA^GG
SexAI        1416  ACCTGGT              A^CC[AT]GGT
Sse8387I     2317  CCTGCAGG             CCTGCA^GG
Tth111I      1397  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         3658  CCATTTAGCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI          501  GAATTT               [GA]^AATT[CT]
             3991  AAATTT              
AflIII       1359  ACGTGT               A^C[GA][CT]GT
             2766  ACGTGT              
Aor51HI      1026  AGCGCT               AGC^GCT
             2328  AGCGCT              
ApoI          501  GAATTT               [GA]^AATT[CT]
             3991  AAATTT              
AviII        1248  TGCGCA               TGC^GCA
             2682  TGCGCA              
BbrPI        2765  CACGTG               CAC^GTG
             3751  CACGTG              
BlpI         2109  GCTCAGC              GC^T[ACGT]AGC
             3333  GCTCAGC             
Bpu1102I     2109  GCTCAGC              GC^T[ACGT]AGC
             3333  GCTCAGC             
BsaAI        2765  CACGTG               [CT]AC^GT[GA]
             3751  CACGTG              
BsaWI        1033  TCCGGA               [AT]^CCGG[AT]
             1492  ACCGGA              
BsmI         2560  GAATGC               GAATGC(1/-1)
             2975  GAATGC              
CelII        2109  GCTCAGC              GC^T[ACGT]AGC
             3333  GCTCAGC             
Eco47III     1026  AGCGCT               AGC^GCT
             2328  AGCGCT              
FspI         1248  TGCGCA               TGC^GCA
             2682  TGCGCA              
PmaCI        2765  CACGTG               CAC^GTG
             3751  CACGTG              
PmlI         2765  CACGTG               CAC^GTG
             3751  CACGTG              
SapI         2216  GAAGAGC              GCTCTTC(1/4)
             1311  GCTCTTC             
SgrAI         805  CGCCGGCG             C[GA]^CCGG[CT]G
             1727  CGCCGGCG            


#########################################
# 3 OR MORE CUT ENZYMES (  136 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmaCI       
XmaI