##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI ApoI AscI Asp718I
AspI AviII AvrII BbeI BfrI
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI DraIII EcoRI EcoRV FseI
FspI HpaI KasI KpnI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
SalI SbfI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 761 GACGTC GACGT^C
AhdI 3647 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 476 AGCGCT AGC^GCT
AseI 1003 ATTAAT AT^TAAT
AsnI 1003 ATTAAT AT^TAAT
AspEI 3647 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 3517 CACGTG CAC^GTG
BciVI 1220 GTATCC GTATCC(6/5)
BclI 2580 TGATCA T^GATCA
BglII 3698 AGATCT A^GATCT
BsaBI 661 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 51 TGTACA T^GTACA
BspHI 2577 TCATGA T^CATGA
BsrGI 51 TGTACA T^GTACA
BstAPI 539 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 400 GGTCACC G^GT[ACGT]ACC
BstPI 400 GGTCACC G^GT[ACGT]ACC
CpoI 857 CGGACCG CG^G[AT]CCG
DraI 2966 TTTAAA TTT^AAA
DrdI 3286 GACATGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3647 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 476 AGCGCT AGC^GCT
EcoO65I 400 GGTCACC G^GT[ACGT]ACC
EcoT22I 2703 ATGCAT ATGCA^T
FbaI 2580 TGATCA T^GATCA
HaeII 476 AGCGCT [GA]GCGC^[CT]
HincII 65 GTTGAC GT[CT]^[GA]AC
HindII 65 GTTGAC GT[CT]^[GA]AC
HindIII 1810 AAGCTT A^AGCTT
MamI 661 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 2703 ATGCAT ATGCA^T
NspI 1105 GCATGT [GA]CATG^[CT]
PaeR7I 2643 CTCGAG C^TCGAG
PmaCI 3517 CACGTG CAC^GTG
PmlI 3517 CACGTG CAC^GTG
PshBI 1003 ATTAAT AT^TAAT
RcaI 2577 TCATGA T^CATGA
RsrII 857 CGGACCG CG^G[AT]CCG
SapI 2548 GCTCTTC GCTCTTC(1/4)
ScaI 2028 AGTACT AGT^ACT
SexAI 2529 ACCAGGT A^CC[AT]GGT
SfiI 1282 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 51 TGTACA T^GTACA
SspI 1000 AATATT AAT^ATT
Tsp509I 4120 AATT ^AATT
XhoI 2643 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AflIII 30 ACGTGT A^C[GA][CT]GT
3518 ACGTGT
Alw44I 1882 GTGCAC G^TGCAC
2816 GTGCAC
ApaLI 1882 GTGCAC G^TGCAC
2816 GTGCAC
BcgI 1162 CGAAGTGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2808 CGAGCCTCGTGC
BsaAI 29 TACGTG [CT]AC^GT[GA]
3517 CACGTG
BsmI 2303 GCATTC GAATGC(1/-1)
2845 GCATTC
BstXI 1563 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3733 CCACTTTCATGG
KspI 247 CCGCGG CCGC^GG
2370 CCGCGG
NaeI 1957 GCCGGC GCC^GGC
3781 GCCGGC
NgoMIV 1957 GCCGGC G^CCGGC
3781 GCCGGC
SacI 1633 GAGCTC GAGCT^C
3396 GAGCTC
SacII 247 CCGCGG CCGC^GG
2370 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI Asp700I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshAI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI