##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AspI
AvrII BbrPI BbvCI BclI BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsiEI BsiWI BspDI BspEI BssHII
CelII ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FbaI FseI HpaI KspI MluI
MroI NcoI NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 3983 CGCG CG^CG
AcsI 2017 GAATTC [GA]^AATT[CT]
ApaI 4186 GGGCCC GGGCC^C
ApoI 2017 GAATTC [GA]^AATT[CT]
Asp700I 1642 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2715 TGCGCA TGC^GCA
BamHI 587 GGATCC G^GATCC
BbeI 11 GGCGCC GGCGC^C
BcgI 1077 GCAGGAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 2425 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1594 GCATTC GAATGC(1/-1)
BspHI 4077 TCATGA T^CATGA
BssSI 908 CTCGTG CACGAG(-5/-1)
BstBI 2084 TTCGAA TT^CGAA
BstEII 2967 GGTCACC G^GT[ACGT]ACC
BstPI 2967 GGTCACC G^GT[ACGT]ACC
BstUI 3983 CGCG CG^CG
DraI 865 TTTAAA TTT^AAA
DrdI 3428 GACTAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2967 GGTCACC G^GT[ACGT]ACC
EcoRI 2017 GAATTC G^AATTC
FspI 2715 TGCGCA TGC^GCA
HgaI 481 GACGC GACGC(5/10)
HincII 502 GTCAAC GT[CT]^[GA]AC
HindII 502 GTCAAC GT[CT]^[GA]AC
HindIII 1957 AAGCTT A^AGCTT
KasI 11 GGCGCC G^GCGCC
MamI 2425 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 115 CAATTG C^AATTG
MunI 115 CAATTG C^AATTG
MvnI 3983 CGCG CG^CG
NaeI 3560 GCCGGC GCC^GGC
NarI 11 GGCGCC GG^CGCC
NdeI 1856 CATATG CA^TATG
NgoMIV 3560 GCCGGC G^CCGGC
NspV 2084 TTCGAA TT^CGAA
PshAI 1893 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1703 CTGCAG CTGCA^G
RcaI 4077 TCATGA T^CATGA
ScaI 1256 AGTACT AGT^ACT
SexAI 2049 ACCTGGT A^CC[AT]GGT
SfoI 11 GGCGCC GGC^GCC
SfuI 2084 TTCGAA TT^CGAA
SgrAI 3559 CGCCGGCG C[GA]^CCGG[CT]G
XmnI 1642 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AatII 712 GACGTC GACGT^C
1372 GACGTC
AflII 1588 CTTAAG C^TTAAG
3504 CTTAAG
AseI 2146 ATTAAT AT^TAAT
3028 ATTAAT
AsnI 2146 ATTAAT AT^TAAT
3028 ATTAAT
BciVI 3884 GGATAC GTATCC(6/5)
3615 GTATCC
BfrI 1588 CTTAAG C^TTAAG
3504 CTTAAG
BglII 1613 AGATCT A^GATCT
2372 AGATCT
BsaWI 590 TCCGGT [AT]^CCGG[AT]
1391 ACCGGA
BsmBI 3255 CGTCTC CGTCTC(1/5)
3648 CGTCTC
Bsp1407I 971 TGTACA T^GTACA
3015 TGTACA
BspMI 3537 ACCTGC ACCTGC(4/8)
4195 GCAGGT
BsrBI 1200 CCGCTC CCGCTC(-3/-3)
2137 GAGCGG
BsrFI 759 GCCGGT [GA]^CCGG[CT]
3560 GCCGGC
BsrGI 971 TGTACA T^GTACA
3015 TGTACA
Bst1107I 1524 GTATAC GTA^TAC
2302 GTATAC
BstAPI 904 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3489 GCACCCAGTGC
BstZ17I 1524 GTATAC GTA^TAC
2302 GTATAC
Cfr10I 759 GCCGGT [GA]^CCGG[CT]
3560 GCCGGC
DsaI 3057 CCGTGG C^C[GA][CT]GG
3824 CCACGG
HaeII 11 GGCGCC [GA]GCGC^[CT]
167 AGCGCC
PshBI 2146 ATTAAT AT^TAAT
3028 ATTAAT
PvuII 3736 CAGCTG CAG^CTG
4034 CAGCTG
SfcI 1703 CTGCAG C^T[GA][CT]AG
1804 CTACAG
SmaI 3317 CCCGGG CCC^GGG
3400 CCCGGG
SmlI 1588 CTTAAG C^T[CT][GA]AG
3504 CTTAAG
SspBI 971 TGTACA T^GTACA
3015 TGTACA
XbaI 1056 TCTAGA T^CTAGA
2361 TCTAGA
XcmI 2237 CCAACCGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3048 CCACATCATCCGTGG
XmaCI 3317 CCCGGG C^CCGGG
3400 CCCGGG
XmaI 3317 CCCGGG C^CCGGG
3400 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
Acc65I AccI AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII