##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BfrI        BlnI        BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BspLU11I    BsrGI       BssHII      
BstBI       ClaI        CpoI        Eam1105I    Eco47III    
EcoRI       EcoRV       FseI        FspI        HgaI        
KasI        KpnI        KspI        MamI        MluI        
NarI        NdeI        NotI        NruI        NspV        
PacI        PflFI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII       2594  TCCGGA               T^CCGGA
AgeI         1758  ACCGGT               A^CCGGT
BbrPI        1209  CACGTG               CAC^GTG
BbsI         1833  GAAGAC               GAAGAC(2/6)
BcgI         4206  GCACTAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2180  GTATCC               GTATCC(6/5)
BclI         4079  TGATCA               T^GATCA
BglI          739  GCCGGCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2699  AGATCT               A^GATCT
BlpI         2215  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2215  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1833  GAAGAC               GAAGAC(2/6)
BsaAI        1209  CACGTG               [CT]AC^GT[GA]
BseAI        2594  TCCGGA               T^CCGGA
BsiEI         737  CGGCCG               CG[GA][CT]^CG
BsmBI        1628  CGTCTC               CGTCTC(1/5)
BspEI        2594  TCCGGA               T^CCGGA
BspHI        3346  TCATGA               T^CATGA
BsrBI         801  CCGCTC               CCGCTC(-3/-3)
BssSI        2054  CACGAG               CACGAG(-5/-1)
Bst1107I     3137  GTATAC               GTA^TAC
BstAPI       1664  GCACAGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      3137  GTATAC               GTA^TAC
CelII        2215  GCTGAGC              GC^T[ACGT]AGC
DrdI          127  GACATTGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          737  CGGCCG               C^GGCCG
EclXI         737  CGGCCG               C^GGCCG
Eco52I        737  CGGCCG               C^GGCCG
EcoNI        1580  CCTGGCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      2853  ATGCAT               ATGCA^T
FbaI         4079  TGATCA               T^GATCA
HindIII      3180  AAGCTT               A^AGCTT
HpaI         3194  GTTAAC               GTT^AAC
MroI         2594  TCCGGA               T^CCGGA
NaeI          739  GCCGGC               GCC^GGC
NgoMIV        739  GCCGGC               G^CCGGC
NheI         1391  GCTAGC               G^CTAGC
NsiI         2853  ATGCAT               ATGCA^T
NspI         1204  ACATGC               [GA]CATG^[CT]
PaeR7I       2616  CTCGAG               C^TCGAG
PinAI        1758  ACCGGT               A^CCGGT
PmaCI        1209  CACGTG               CAC^GTG
PmlI         1209  CACGTG               CAC^GTG
RcaI         3346  TCATGA               T^CATGA
SexAI        3198  ACCAGGT              A^CC[AT]GGT
SfiI          738  GGCCGGCTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI         2617  TCGA                 T^CGA
TthHB8I      2617  TCGA                 T^CGA
XbaI         3587  TCTAGA               T^CTAGA
XhoI         2616  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AatII        1626  GACGTC               GACGT^C
             1920  GACGTC              
AccI         3137  GTATAC               GT^[AC][GT]AC
             3567  GTAGAC              
AcyI         1626  GACGTC               G[GA]^CG[CT]C
             1920  GACGTC              
AlwNI        1846  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3200  CAGGTCCTG           
AocI         4085  CCTGAGG              CC^T[ACGT]AGG
             4274  CCTGAGG             
BanI         1477  GGCACC               G^G[CT][GA]CC
             2286  GGTGCC              
BsaHI        1626  GACGTC               G[GA]^CG[CT]C
             1920  GACGTC              
BsgI         3963  CTGCAC               GTGCAG(16/14)
             1267  GTGCAG              
BstEII        603  GGTGACC              G^GT[ACGT]ACC
             3546  GGTCACC             
BstPI         603  GGTGACC              G^GT[ACGT]ACC
             3546  GGTCACC             
Bsu36I       4085  CCTGAGG              CC^T[ACGT]AGG
             4274  CCTGAGG             
EarI         3422  CTCTTC               CTCTTC(1/4)
             3432  GAAGAG              
Eco81I       4085  CCTGAGG              CC^T[ACGT]AGG
             4274  CCTGAGG             
EcoO65I       603  GGTGACC              G^GT[ACGT]ACC
             3546  GGTCACC             
Hin1I        1626  GACGTC               G[GA]^CG[CT]C
             1920  GACGTC              
Ksp632I      3422  CTCTTC               CTCTTC(1/4)
             3432  GAAGAG              
MfeI         2314  CAATTG               C^AATTG
             3259  CAATTG              
MunI         2314  CAATTG               C^AATTG
             3259  CAATTG              
NcoI          457  CCATGG               C^CATGG
             2719  CCATGG              
SmaI          718  CCCGGG               CCC^GGG
             3228  CCCGGG              
XmaCI         718  CCCGGG               C^CCGGG
             3228  CCCGGG              
XmaI          718  CCCGGG               C^CCGGG
             3228  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaI        AvaII       
BalI        BanII       BbvCI       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BsaWI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraI        DraII       DraIII      DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XhoII