##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI Alw44I ApaLI AscI AseI
AsnI AspI BbrPI BciVI BclI
BglII BsaBI BsiWI Bsp1407I BspDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI EcoO65I EcoRV
EcoT22I FbaI FseI HgaI HindIII
HpaI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmlI PshAI PshBI Psp1406I PvuI
SbfI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 9 GACGTC GACGT^C
Acc65I 3316 GGTACC G^GTACC
AccIII 2606 TCCGGA T^CCGGA
AflII 2596 CTTAAG C^TTAAG
AflIII 3224 ACATGT A^C[GA][CT]GT
AhdI 3636 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2626 CCTAAGG CC^T[ACGT]AGG
ApaI 1001 GGGCCC GGGCC^C
Asp700I 2632 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3316 GGTACC G^GTACC
AspEI 3636 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 55 TGCGCA TGC^GCA
AvrII 99 CCTAGG C^CTAGG
BalI 3324 TGGCCA TGG^CCA
BamHI 2622 GGATCC G^GATCC
BbeI 862 GGCGCC GGCGC^C
BfrI 2596 CTTAAG C^TTAAG
BlnI 99 CCTAGG C^CTAGG
BlpI 2205 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2205 GCTGAGC GC^T[ACGT]AGC
BsaAI 767 TACGTG [CT]AC^GT[GA]
BsaI 1997 GAGACC GGTCTC(1/5)
BseAI 2606 TCCGGA T^CCGGA
BsmBI 2778 CGTCTC CGTCTC(1/5)
BsmI 3101 GAATGC GAATGC(1/-1)
BspEI 2606 TCCGGA T^CCGGA
BspHI 2838 TCATGA T^CATGA
BspLU11I 3224 ACATGT A^CATGT
BspMI 536 GCAGGT ACCTGC(4/8)
BssSI 945 CACGAG CACGAG(-5/-1)
Bsu36I 2626 CCTAAGG CC^T[ACGT]AGG
CelII 2205 GCTGAGC GC^T[ACGT]AGC
CpoI 321 CGGTCCG CG^G[AT]CCG
DraIII 1510 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3148 GACAGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3636 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2626 CCTAAGG CC^T[ACGT]AGG
FspI 55 TGCGCA TGC^GCA
KasI 862 GGCGCC G^GCGCC
KpnI 3316 GGTACC GGTAC^C
MluNI 3324 TGGCCA TGG^CCA
MroI 2606 TCCGGA T^CCGGA
MscI 3324 TGGCCA TGG^CCA
NarI 862 GGCGCC GG^CGCC
NdeI 3422 CATATG CA^TATG
NruI 967 TCGCGA TCG^CGA
PflMI 3865 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3255 GTTTAAAC GTTT^AAAC
PstI 2978 CTGCAG CTGCA^G
RcaI 2838 TCATGA T^CATGA
RsrII 321 CGGTCCG CG^G[AT]CCG
SacI 2917 GAGCTC GAGCT^C
SalI 1231 GTCGAC G^TCGAC
SfoI 862 GGCGCC GGC^GCC
SgrAI 1584 CACCGGTG C[GA]^CCGG[CT]G
SmaI 114 CCCGGG CCC^GGG
SspI 2658 AATATT AAT^ATT
Van91I 3865 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 114 CCCGGG C^CCGGG
XmaI 114 CCCGGG C^CCGGG
XmnI 2632 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 9 GACGTC G[GA]^CG[CT]C
862 GGCGCC
AgeI 1585 ACCGGT A^CCGGT
3519 ACCGGT
AspHI 2917 GAGCTC G[AT]GC[AT]^C
3904 GAGCAC
BcgI 341 CGAAGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1140 GCATCGGGGTCG
BglI 726 GCCTTTTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3025 GCCCTGGTGGC
BsaHI 9 GACGTC G[GA]^CG[CT]C
862 GGCGCC
BsiHKAI 2917 GAGCTC G[AT]GC[AT]^C
3904 GAGCAC
BstXI 2310 CCAAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3436 CCAGTACCCTGG
EcoRI 2570 GAATTC G^AATTC
3789 GAATTC
Hin1I 9 GACGTC G[GA]^CG[CT]C
862 GGCGCC
KspI 207 CCGCGG CCGC^GG
215 CCGCGG
NcoI 2617 CCATGG C^CATGG
3321 CCATGG
PinAI 1585 ACCGGT A^CCGGT
3519 ACCGGT
SacII 207 CCGCGG CCGC^GG
215 CCGCGG
SapI 684 GAAGAGC GCTCTTC(1/4)
2914 GAAGAGC
ScaI 3372 AGTACT AGT^ACT
3532 AGTACT
StuI 724 AGGCCT AGG^CCT
2592 AGGCCT
XcmI 1564 CCACTAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1647 CCAGGGGATTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI Aor51HI ApoI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrDI
BsrFI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII