##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AscI AspI AviII AvrII
BbeI BbrPI BcgI BfrI BlnI
BsaAI BseAI BsiEI BsiWI BsmBI
BspEI BssHII BstBI BstUI CpoI
DraIII DrdI EagI EclXI Eco52I
FseI FspI HpaI KasI KspI
MluI MroI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1848 GGTACC G^GTACC
AcyI 4372 GACGCC G[GA]^CG[CT]C
AhdI 4372 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2660 GTGCAC G^TGCAC
AocI 1714 CCTAAGG CC^T[ACGT]AGG
Aor51HI 3854 AGCGCT AGC^GCT
ApaI 1427 GGGCCC GGGCC^C
ApaLI 2660 GTGCAC G^TGCAC
Asp718I 1848 GGTACC G^GTACC
AspEI 4372 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3556 GGATCC G^GATCC
BciVI 2725 GTATCC GTATCC(6/5)
BsaHI 4372 GACGCC G[GA]^CG[CT]C
BsaWI 4166 ACCGGA [AT]^CCGG[AT]
Bsp1407I 6675 TGTACA T^GTACA
BspDI 2953 ATCGAT AT^CGAT
BspHI 984 TCATGA T^CATGA
BsrFI 4991 ACCGGC [GA]^CCGG[CT]
BsrGI 6675 TGTACA T^GTACA
Bst1107I 2138 GTATAC GTA^TAC
BstZ17I 2138 GTATAC GTA^TAC
Bsu36I 1714 CCTAAGG CC^T[ACGT]AGG
Cfr10I 4991 ACCGGC [GA]^CCGG[CT]
ClaI 2953 ATCGAT AT^CGAT
Eam1105I 4372 GACGCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3854 AGCGCT AGC^GCT
Eco81I 1714 CCTAAGG CC^T[ACGT]AGG
FauI 5612 CCCGC CCCGC(4/6)
HgaI 4372 GACGC GACGC(5/10)
Hin1I 4372 GACGCC G[GA]^CG[CT]C
HincII 6954 GTCGAC GT[CT]^[GA]AC
HindII 6954 GTCGAC GT[CT]^[GA]AC
KpnI 1848 GGTACC GGTAC^C
NdeI 12 CATATG CA^TATG
PflMI 3513 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4704 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 984 TCATGA T^CATGA
SalI 6954 GTCGAC G^TCGAC
SphI 863 GCATGC GCATG^C
SspBI 6675 TGTACA T^GTACA
StuI 3387 AGGCCT AGG^CCT
Van91I 3513 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2138 GTATAC GT^[AC][GT]AC
6954 GTCGAC
AseI 1525 ATTAAT AT^TAAT
6635 ATTAAT
AsnI 1525 ATTAAT AT^TAAT
6635 ATTAAT
BclI 16 TGATCA T^GATCA
6642 TGATCA
BglI 5356 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5368 GCCATTGAGGC
BglII 1656 AGATCT A^GATCT
6378 AGATCT
BsaBI 2481 GATGCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5034 GATTCAAATC
BsrBI 4398 CCGCTC CCGCTC(-3/-3)
5116 GAGCGG
BssSI 4471 CACGAG CACGAG(-5/-1)
6507 CACGAG
BstAPI 4347 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5898 GCATCCCCTGC
EcoRI 3363 GAATTC G^AATTC
6583 GAATTC
EcoRV 886 GATATC GAT^ATC
3055 GATATC
EcoT22I 2106 ATGCAT ATGCA^T
5628 ATGCAT
FbaI 16 TGATCA T^GATCA
6642 TGATCA
HaeII 431 GGCGCT [GA]GCGC^[CT]
3854 AGCGCT
HindIII 1646 AAGCTT A^AGCTT
4816 AAGCTT
MaeII 79 ACGT A^CGT
4749 ACGT
MamI 2481 GATGCGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5034 GATTCAAATC
MfeI 1560 CAATTG C^AATTG
7031 CAATTG
MunI 1560 CAATTG C^AATTG
7031 CAATTG
NsiI 2106 ATGCAT ATGCA^T
5628 ATGCAT
PaeR7I 3476 CTCGAG C^TCGAG
3700 CTCGAG
PshBI 1525 ATTAAT AT^TAAT
6635 ATTAAT
ScaI 1969 AGTACT AGT^ACT
6809 AGTACT
SexAI 2133 ACCAGGT A^CC[AT]GGT
4634 ACCAGGT
XhoI 3476 CTCGAG C^TCGAG
3700 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI