##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BlnI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DraI Eam1105I EcoRV FseI
FspI HincII HindII HindIII HpaI
KasI KpnI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SpeI Sse8387I
SspBI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 614 GACGCC G[GA]^CG[CT]C
AflII 2071 CTTAAG C^TTAAG
AocI 2982 CCTTAGG CC^T[ACGT]AGG
AspI 3231 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2576 GCTGAGG CCTCAGC(-5/-2)
BcgI 1090 GCACCACGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2816 TGATCA T^GATCA
BfrI 2071 CTTAAG C^TTAAG
BlpI 1111 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1111 GCTCAGC GC^T[ACGT]AGC
BsaAI 3570 TACGTA [CT]AC^GT[GA]
BsaBI 322 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 614 GACGCC G[GA]^CG[CT]C
BsmI 171 GAATGC GAATGC(1/-1)
BssHII 1053 GCGCGC G^CGCGC
BstEII 439 GGTCACC G^GT[ACGT]ACC
BstPI 439 GGTCACC G^GT[ACGT]ACC
Bsu36I 2982 CCTTAGG CC^T[ACGT]AGG
CelII 1111 GCTCAGC GC^T[ACGT]AGC
DraIII 1437 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 647 GACCTGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1079 CGGCCG C^GGCCG
EclXI 1079 CGGCCG C^GGCCG
Eco52I 1079 CGGCCG C^GGCCG
Eco81I 2982 CCTTAGG CC^T[ACGT]AGG
EcoO65I 439 GGTCACC G^GT[ACGT]ACC
FbaI 2816 TGATCA T^GATCA
Hin1I 614 GACGCC G[GA]^CG[CT]C
MamI 322 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1262 CCATGG C^CATGG
NheI 2196 GCTAGC G^CTAGC
PflFI 3231 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
ScaI 3499 AGTACT AGT^ACT
SnaBI 3570 TACGTA TAC^GTA
SspI 2606 AATATT AAT^ATT
StuI 2339 AGGCCT AGG^CCT
Tth111I 3231 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Aor51HI 1071 AGCGCT AGC^GCT
1115 AGCGCT
AvaI 1428 CCCGAG C^[CT]CG[GA]G
3452 CCCGAG
BalI 363 TGGCCA TGG^CCA
3273 TGGCCA
BamHI 1619 GGATCC G^GATCC
1963 GGATCC
BbsI 1886 GAAGAC GAAGAC(2/6)
693 GTCTTC
BciVI 2582 GGATAC GTATCC(6/5)
3401 GTATCC
BglII 2179 AGATCT A^GATCT
2517 AGATCT
BpuAI 1886 GAAGAC GAAGAC(2/6)
693 GTCTTC
BsaI 1127 GAGACC GGTCTC(1/5)
205 GGTCTC
BsiEI 1079 CGGCCG CG[GA][CT]^CG
1096 CGGTCG
BsoBI 1428 CCCGAG C^[CT]CG[GA]G
3452 CCCGAG
BsrBI 291 CCGCTC CCGCTC(-3/-3)
2627 CCGCTC
BstAPI 1792 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2488 GCAGGGACTGC
Eco47III 1071 AGCGCT AGC^GCT
1115 AGCGCT
EcoRI 62 GAATTC G^AATTC
2508 GAATTC
EcoT22I 2189 ATGCAT ATGCA^T
3177 ATGCAT
HaeII 1071 AGCGCT [GA]GCGC^[CT]
1115 AGCGCT
HgaI 614 GACGC GACGC(5/10)
1669 GACGC
KspI 1082 CCGCGG CCGC^GG
1122 CCGCGG
MluNI 363 TGGCCA TGG^CCA
3273 TGGCCA
MscI 363 TGGCCA TGG^CCA
3273 TGGCCA
NsiI 2189 ATGCAT ATGCA^T
3177 ATGCAT
PstI 1169 CTGCAG CTGCA^G
1790 CTGCAG
SacII 1082 CCGCGG CCGC^GG
1122 CCGCGG
SexAI 807 ACCAGGT A^CC[AT]GGT
3586 ACCTGGT
SphI 621 GCATGC GCATG^C
1107 GCATGC
TaqI 685 TCGA T^CGA
909 TCGA
TthHB8I 685 TCGA T^CGA
909 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmnI