##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccI AflII Alw44I
Aor51HI ApaLI AscI Asp700I Asp718I
AvrII BamHI BbvCI BclI BfrI
BglI BlnI BsaBI BsiWI BssHII
Bst1107I BstBI BstZ17I DrdI EagI
EclXI Eco47III Eco52I FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MluI NheI NotI NruI
NspV PacI PaeR7I PmeI PshAI
PvuI SalI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI StuI
SwaI XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 290 TCCGGA T^CCGGA
AgeI 2766 ACCGGT A^CCGGT
AhdI 2554 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1466 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 2554 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1529 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1296 TGCGCA TGC^GCA
BalI 972 TGGCCA TGG^CCA
BcgI 1422 GCAACAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1173 AGATCT A^GATCT
BlpI 1438 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1438 GCTGAGC GC^T[ACGT]AGC
BseAI 290 TCCGGA T^CCGGA
BsiEI 2580 CGACCG CG[GA][CT]^CG
BspDI 146 ATCGAT AT^CGAT
BspEI 290 TCCGGA T^CCGGA
BspHI 2090 TCATGA T^CATGA
BsrBI 2322 CCGCTC CCGCTC(-3/-3)
BssSI 2365 CACGAG CACGAG(-5/-1)
CelII 1438 GCTGAGC GC^T[ACGT]AGC
ClaI 146 ATCGAT AT^CGAT
CpoI 1946 CGGTCCG CG^G[AT]CCG
Eam1105I 2554 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 964 GAATTC G^AATTC
EcoRV 4153 GATATC GAT^ATC
FspI 1296 TGCGCA TGC^GCA
MluNI 972 TGGCCA TGG^CCA
MroI 290 TCCGGA T^CCGGA
MscI 972 TGGCCA TGG^CCA
NaeI 2635 GCCGGC GCC^GGC
NdeI 4189 CATATG CA^TATG
NgoMIV 2635 GCCGGC G^CCGGC
PflFI 1529 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2766 ACCGGT A^CCGGT
RcaI 2090 TCATGA T^CATGA
RsrII 1946 CGGTCCG CG^G[AT]CCG
SapI 2076 GCTCTTC GCTCTTC(1/4)
SbfI 1573 CCTGCAGG CCTGCA^GG
SgrAI 2564 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 2371 CTCAAG C^T[CT][GA]AG
Sse8387I 1573 CCTGCAGG CCTGCA^GG
Tth111I 1529 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AclI 2845 AACGTT AA^CGTT
2996 AACGTT
ApaI 1967 GGGCCC GGGCC^C
2230 GGGCCC
AseI 2979 ATTAAT AT^TAAT
3961 ATTAAT
AsnI 2979 ATTAAT AT^TAAT
3961 ATTAAT
BbeI 1404 GGCGCC GGCGC^C
1804 GGCGCC
BsmBI 308 CGTCTC CGTCTC(1/5)
2712 GAGACG
BsmI 3478 GCATTC GAATGC(1/-1)
3655 GCATTC
Bsp1407I 4040 TGTACA T^GTACA
4087 TGTACA
BsrDI 366 GCAATG GCAATG(2/0)
4130 GCAATG
BsrGI 4040 TGTACA T^GTACA
4087 TGTACA
BstEII 2376 GGTCACC G^GT[ACGT]ACC
3181 GGTGACC
BstPI 2376 GGTCACC G^GT[ACGT]ACC
3181 GGTGACC
BstXI 1320 CCACGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3531 CCATAGATGTGG
DraIII 1150 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2960 CACACAGTG
EcoNI 1570 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3186 CCTGTGAAAGG
EcoO65I 2376 GGTCACC G^GT[ACGT]ACC
3181 GGTGACC
KasI 1404 GGCGCC G^GCGCC
1804 GGCGCC
KspI 2226 CCGCGG CCGC^GG
2258 CCGCGG
MfeI 3818 CAATTG C^AATTG
3887 CAATTG
MunI 3818 CAATTG C^AATTG
3887 CAATTG
NarI 1404 GGCGCC GG^CGCC
1804 GGCGCC
NcoI 400 CCATGG C^CATGG
3328 CCATGG
PshBI 2979 ATTAAT AT^TAAT
3961 ATTAAT
Psp1406I 2845 AACGTT AA^CGTT
2996 AACGTT
SacI 2148 GAGCTC GAGCT^C
2368 GAGCTC
SacII 2226 CCGCGG CCGC^GG
2258 CCGCGG
SfoI 1404 GGCGCC GGC^GCC
1804 GGCGCC
SmaI 2546 CCCGGG CCC^GGG
2736 CCCGGG
SspBI 4040 TGTACA T^GTACA
4087 TGTACA
SspI 3616 AATATT AAT^ATT
3942 AATATT
XmaCI 2546 CCCGGG C^CCGGG
2736 CCCGGG
XmaI 2546 CCCGGG C^CCGGG
2736 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AocI ApoI
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII