##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AflII AgeI Alw44I ApaLI
AscI AviII AvrII BamHI BbrPI
BfrI BlnI BsaBI BsiWI Bsp1407I
BspDI BspHI BsrFI BsrGI BssHII
BssSI BstEII BstPI Cfr10I ClaI
CpoI DrdI EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KspI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SgrAI Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 3499 GGTACC G^GTACC
AccI 3680 GTATAC GT^[AC][GT]AC
AccII 3847 CGCG CG^CG
AclI 198 AACGTT AA^CGTT
AcyI 1226 GGCGCC G[GA]^CG[CT]C
AflIII 970 ACATGT A^C[GA][CT]GT
AhdI 484 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2975 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 319 AGCGCT AGC^GCT
ApaI 711 GGGCCC GGGCC^C
Asp718I 3499 GGTACC G^GTACC
AspEI 484 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4100 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1226 GGCGCC GGCGC^C
BciVI 344 GTATCC GTATCC(6/5)
BclI 2158 TGATCA T^GATCA
BglII 2969 AGATCT A^GATCT
BsaAI 2606 TACGTA [CT]AC^GT[GA]
BsaHI 1226 GGCGCC G[GA]^CG[CT]C
BsaI 1500 GAGACC GGTCTC(1/5)
BsiEI 887 CGGCCG CG[GA][CT]^CG
BsmI 159 GAATGC GAATGC(1/-1)
BspLU11I 970 ACATGT A^CATGT
BsrBI 882 CCGCTC CCGCTC(-3/-3)
BsrDI 212 GCAATG GCAATG(2/0)
Bst1107I 3680 GTATAC GTA^TAC
BstBI 1831 TTCGAA TT^CGAA
BstUI 3847 CGCG CG^CG
BstZ17I 3680 GTATAC GTA^TAC
EagI 887 CGGCCG C^GGCCG
Eam1105I 484 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 887 CGGCCG C^GGCCG
Eco47III 319 AGCGCT AGC^GCT
Eco52I 887 CGGCCG C^GGCCG
FbaI 2158 TGATCA T^GATCA
HgaI 3020 GACGC GACGC(5/10)
Hin1I 1226 GGCGCC G[GA]^CG[CT]C
HincII 906 GTCAAC GT[CT]^[GA]AC
HindII 906 GTCAAC GT[CT]^[GA]AC
KasI 1226 GGCGCC G^GCGCC
KpnI 3499 GGTACC GGTAC^C
MslI 846 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 3847 CGCG CG^CG
NarI 1226 GGCGCC GG^CGCC
NspV 1831 TTCGAA TT^CGAA
PaeR7I 645 CTCGAG C^TCGAG
PflFI 4100 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1995 AGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 198 AACGTT AA^CGTT
PvuII 254 CAGCTG CAG^CTG
SacI 2807 GAGCTC GAGCT^C
SfoI 1226 GGCGCC GGC^GCC
SfuI 1831 TTCGAA TT^CGAA
SmaI 860 CCCGGG CCC^GGG
SnaBI 2606 TACGTA TAC^GTA
SpeI 2088 ACTAGT A^CTAGT
SphI 130 GCATGC GCATG^C
Tth111I 4100 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 645 CTCGAG C^TCGAG
XmaCI 860 CCCGGG C^CCGGG
XmaI 860 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 1260 CCTCAGG CC^T[ACGT]AGG
1349 CCTCAGG
Asp700I 277 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2172 GAATGACTTC
AvaI 645 CTCGAG C^[CT]CG[GA]G
860 CCCGGG
BalI 1463 TGGCCA TGG^CCA
3930 TGGCCA
BbsI 1446 GTCTTC GAAGAC(2/6)
2787 GTCTTC
BbvCI 875 CCTCAGC CCTCAGC(-5/-2)
4020 GCTGAGG
BcgI 2240 CGAAATCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4026 GCAGGAGAATCG
BlpI 831 GCTGAGC GC^T[ACGT]AGC
1470 GCTTAGC
Bpu1102I 831 GCTGAGC GC^T[ACGT]AGC
1470 GCTTAGC
BpuAI 1446 GTCTTC GAAGAC(2/6)
2787 GTCTTC
BsoBI 645 CTCGAG C^[CT]CG[GA]G
860 CCCGGG
BstAPI 985 GCACACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3033 GCACCCAGTGC
BstYI 657 GGATCT [GA]^GATC[CT]
2969 AGATCT
Bsu36I 1260 CCTCAGG CC^T[ACGT]AGG
1349 CCTCAGG
CelII 831 GCTGAGC GC^T[ACGT]AGC
1470 GCTTAGC
DraIII 1592 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3034 CACCCAGTG
Eco81I 1260 CCTCAGG CC^T[ACGT]AGG
1349 CCTCAGG
EcoRI 2146 GAATTC G^AATTC
4041 GAATTC
HaeII 319 AGCGCT [GA]GCGC^[CT]
1226 GGCGCC
MflI 657 GGATCT [GA]^GATC[CT]
2969 AGATCT
MluNI 1463 TGGCCA TGG^CCA
3930 TGGCCA
MscI 1463 TGGCCA TGG^CCA
3930 TGGCCA
NcoI 1460 CCATGG C^CATGG
1924 CCATGG
XbaI 1210 TCTAGA T^CTAGA
2960 TCTAGA
XhoII 657 GGATCT [GA]^GATC[CT]
2969 AGATCT
XmnI 277 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2172 GAATGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccIII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
Bsp1286I BspEI BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MroI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI