##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       AccIII      AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AspEI       AvrII       BbeI        BbrPI       
BcgI        BciVI       BfrI        BlnI        BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BssHII      BstEII      BstPI       BstXI       
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
FseI        HpaI        KasI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NotI        NruI        PacI        
PinAI       PmaCI       PmeI        PmlI        PvuI        
RsrII       SacI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        StuI        SwaI        
XbaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AclI           17  AACGTT               AA^CGTT
AcyI          400  GGCGTC               G[GA]^CG[CT]C
AflIII       3209  ACGTGT               A^C[GA][CT]GT
AseI         4291  ATTAAT               AT^TAAT
AsnI         4291  ATTAAT               AT^TAAT
AspI         3099  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         164  TGCGCA               TGC^GCA
BalI         3447  TGGCCA               TGG^CCA
BamHI         139  GGATCC               G^GATCC
BbvCI        1111  CCTCAGC              CCTCAGC(-5/-2)
BlpI         1166  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1166  GCTCAGC              GC^T[ACGT]AGC
BsaHI         400  GGCGTC               G[GA]^CG[CT]C
BsaI          453  GGTCTC               GGTCTC(1/5)
BspHI          93  TCATGA               T^CATGA
BspMI        3415  GCAGGT               ACCTGC(4/8)
BsrDI        1522  GCAATG               GCAATG(2/0)
Bst1107I     2107  GTATAC               GTA^TAC
BstAPI       1870  GCATTCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      2107  GTATAC               GTA^TAC
CelII        1166  GCTCAGC              GC^T[ACGT]AGC
EcoNI        1371  CCTCTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1428  GAATTC               G^AATTC
FspI          164  TGCGCA               TGC^GCA
HaeII        1401  AGCGCC               [GA]GCGC^[CT]
HgaI          401  GCGTC                GACGC(5/10)
Hin1I         400  GGCGTC               G[GA]^CG[CT]C
KspI         3584  CCGCGG               CCGC^GG
MluNI        3447  TGGCCA               TGG^CCA
MscI         3447  TGGCCA               TGG^CCA
NheI         2940  GCTAGC               G^CTAGC
PaeR7I       1330  CTCGAG               C^TCGAG
PflFI        3099  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        3334  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4291  ATTAAT               AT^TAAT
Psp1406I       17  AACGTT               AA^CGTT
RcaI           93  TCATGA               T^CATGA
SacII        3584  CCGCGG               CCGC^GG
SalI         2042  GTCGAC               G^TCGAC
Tth111I      3099  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1330  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Acc65I        737  GGTACC               G^GTACC
             3480  GGTACC              
AccII         324  CGCG                 CG^CG
             3585  CGCG                
Asp718I       737  GGTACC               G^GTACC
             3480  GGTACC              
BanII        1164  GGGCTC               G[GA]GC[CT]^C
             3344  GAGCCC              
BglII         428  AGATCT               A^GATCT
             3878  AGATCT              
BssSI         520  CTCGTG               CACGAG(-5/-1)
             1159  CTCGTG              
BstBI         251  TTCGAA               TT^CGAA
              345  TTCGAA              
BstUI         324  CGCG                 CG^CG
             3585  CGCG                
EaeI         1060  CGGCCA               [CT]^GGCC[GA]
             3447  TGGCCA              
EcoT22I       628  ATGCAT               ATGCA^T
             3925  ATGCAT              
FauI          396  GCGGG                CCCGC(4/6)
             1047  GCGGG               
KpnI          737  GGTACC               GGTAC^C
             3480  GGTACC              
MslI          262  CATACACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4026  CACAGAGATG          
MvnI          324  CGCG                 CG^CG
             3585  CGCG                
NcoI           84  CCATGG               C^CATGG
              919  CCATGG              
NsiI          628  ATGCAT               ATGCA^T
             3925  ATGCAT              
NspV          251  TTCGAA               TT^CGAA
              345  TTCGAA              
SfuI          251  TTCGAA               TT^CGAA
              345  TTCGAA              
SmaI         1172  CCCGGG               CCC^GGG
             2680  CCCGGG              
SphI         1446  GCATGC               GCATG^C
             2615  GCATGC              
XcmI         2039  CCAGTCGACGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3354  CCAGGCAATCTTTGG     
XmaCI        1172  CCCGGG               C^CCGGG
             2680  CCCGGG              
XmaI         1172  CCCGGG               C^CCGGG
             2680  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BanI        BbsI        BbvI        
BclI        BcnI        BfaI        BglI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       XmnI