##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BglI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspMI       BsrBI       BsrGI       BssSI       
BstAPI      BstBI       BstEII      BstPI       BstXI       
Bsu36I      CelII       ClaI        CpoI        DraIII      
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRV       FseI        FspI        Hin1I       
HindIII     KasI        KpnI        MamI        MluI        
MroI        NarI        NciI        NcoI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         3643  GTATAC               GT^[AC][GT]AC
AflII        2038  CTTAAG               C^TTAAG
Alw44I       3164  GTGCAC               G^TGCAC
AlwI         2347  GATCC                GGATC(4/5)
AlwNI          77  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        3164  GTGCAC               G^TGCAC
Asp700I      1256  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        3164  GTGCAC               G[AT]GC[AT]^C
AvaI          116  CCCGAG               C^[CT]CG[GA]G
AvaII        1561  GGACC                G^G[AT]CC
AvrII        3582  CCTAGG               C^CTAGG
BanII        1676  GAGCCC               G[GA]GC[CT]^C
BciVI        1821  GTATCC               GTATCC(6/5)
BclI         2829  TGATCA               T^GATCA
BfrI         2038  CTTAAG               C^TTAAG
BlnI         3582  CCTAGG               C^CTAGG
BpmI          535  CTCCAG               CTGGAG(16/14)
BsaI          244  GGTCTC               GGTCTC(1/5)
BsiEI         239  CGGCCG               CG[GA][CT]^CG
BsiHKAI      3164  GTGCAC               G[AT]GC[AT]^C
BsmI         3856  GCATTC               GAATGC(1/-1)
BsoBI         116  CCCGAG               C^[CT]CG[GA]G
BspHI         420  TCATGA               T^CATGA
BssHII        133  GCGCGC               G^CGCGC
Bst1107I     3643  GTATAC               GTA^TAC
BstZ17I      3643  GTATAC               GTA^TAC
DsaI          184  CCGCGG               C^C[GA][CT]GG
EagI          239  CGGCCG               C^GGCCG
EclXI         239  CGGCCG               C^GGCCG
Eco52I        239  CGGCCG               C^GGCCG
EcoRI        3546  GAATTC               G^AATTC
EcoT14I      3582  CCTAGG               C^C[AT][AT]GG
FbaI         2829  TGATCA               T^GATCA
KspI          184  CCGCGG               CCGC^GG
MaeII         880  ACGT                 A^CGT
MfeI          348  CAATTG               C^AATTG
MslI         2833  CATTTATATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          348  CAATTG               C^AATTG
NaeI           16  GCCGGC               GCC^GGC
NgoMIV         16  GCCGGC               G^CCGGC
PflMI         729  CCAACAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1560  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          420  TCATGA               T^CATGA
SacII         184  CCGCGG               CCGC^GG
SapI         1358  GAAGAGC              GCTCTTC(1/4)
SmlI         2038  CTTAAG               C^T[CT][GA]AG
StyI         3582  CCTAGG               C^C[AT][AT]GG
Van91I        729  CCAACAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1256  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BalI         1879  TGGCCA               TGG^CCA
             2154  TGGCCA              
BanI         2286  GGCACC               G^G[CT][GA]CC
             3714  GGTGCC              
BbsI          602  GAAGAC               GAAGAC(2/6)
             2301  GTCTTC              
BmyI         1676  GAGCCC               G[AGT]GC[ACT]^C
             3164  GTGCAC              
BpuAI         602  GAAGAC               GAAGAC(2/6)
             2301  GTCTTC              
BsmFI        1797  GGGAC                GGGAC(10/14)
               79  GTCCC               
Bsp1286I     1676  GAGCCC               G[AGT]GC[ACT]^C
             3164  GTGCAC              
BsrFI          16  GCCGGC               [GA]^CCGG[CT]
              241  GCCGGT              
Cfr10I         16  GCCGGC               [GA]^CCGG[CT]
              241  GCCGGT              
EcoT22I      1521  ATGCAT               ATGCA^T
             3854  ATGCAT              
HaeII         120  AGCGCC               [GA]GCGC^[CT]
              127  AGCGCC              
HapII          17  CCGG                 C^CGG
              242  CCGG                
HgaI          891  GACGC                GACGC(5/10)
             2116  GCGTC               
HpaI         2865  GTTAAC               GTT^AAC
             3074  GTTAAC              
HpaII          17  CCGG                 C^CGG
              242  CCGG                
MluNI        1879  TGGCCA               TGG^CCA
             2154  TGGCCA              
MscI         1879  TGGCCA               TGG^CCA
             2154  TGGCCA              
MspI           17  CCGG                 C^CGG
              242  CCGG                
NdeI         1916  CATATG               CA^TATG
             3188  CATATG              
NsiI         1521  ATGCAT               ATGCA^T
             3854  ATGCAT              
PstI          147  CTGCAG               CTGCA^G
             3653  CTGCAG              
ScaI          437  AGTACT               AGT^ACT
             3921  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        ApoI        BbvI        BfaI        BglII       
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BspLU11I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HincII      HindII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII