##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspHI AspI AvrII BbeI BglI
BlnI BsaHI BsaI BseRI BsiEI
BsiHKAI BsiWI BsmBI BspDI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I FseI HgaI Hin1I KasI
KspI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PinAI
PpuMI PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 3501 GGTACC G^GTACC
AccII 81 CGCG CG^CG
AccIII 3104 TCCGGA T^CCGGA
AclI 2942 AACGTT AA^CGTT
AflII 404 CTTAAG C^TTAAG
AflIII 3401 ACGTGT A^C[GA][CT]GT
AocI 730 CCTTAGG CC^T[ACGT]AGG
Asp718I 3501 GGTACC G^GTACC
AviII 2308 TGCGCA TGC^GCA
BalI 3197 TGGCCA TGG^CCA
BbrPI 3400 CACGTG CAC^GTG
BbsI 1822 GTCTTC GAAGAC(2/6)
BbvCI 2748 CCTCAGC CCTCAGC(-5/-2)
BcgI 656 GCAGAATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 404 CTTAAG C^TTAAG
BpuAI 1822 GTCTTC GAAGAC(2/6)
BsaBI 3108 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3104 TCCGGA [AT]^CCGG[AT]
BseAI 3104 TCCGGA T^CCGGA
BsmAI 3246 GAGAC GTCTC(1/5)
BspEI 3104 TCCGGA T^CCGGA
BspHI 2956 TCATGA T^CATGA
BspMI 2598 GCAGGT ACCTGC(4/8)
BsrFI 928 ACCGGC [GA]^CCGG[CT]
BstUI 81 CGCG CG^CG
Bsu36I 730 CCTTAGG CC^T[ACGT]AGG
Cfr10I 928 ACCGGC [GA]^CCGG[CT]
EaeI 3197 TGGCCA [CT]^GGCC[GA]
Eco81I 730 CCTTAGG CC^T[ACGT]AGG
EcoNI 1521 CCTACGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2205 GATATC GAT^ATC
EcoT22I 2715 ATGCAT ATGCA^T
FspI 2308 TGCGCA TGC^GCA
KpnI 3501 GGTACC GGTAC^C
MamI 3108 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3197 TGGCCA TGG^CCA
MroI 3104 TCCGGA T^CCGGA
MscI 3197 TGGCCA TGG^CCA
MvnI 81 CGCG CG^CG
NsiI 2715 ATGCAT ATGCA^T
PacI 3982 TTAATTAA TTAAT^TAA
PflMI 2524 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3400 CACGTG CAC^GTG
PmeI 1720 GTTTAAAC GTTT^AAAC
PmlI 3400 CACGTG CAC^GTG
PshAI 1815 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2942 AACGTT AA^CGTT
RcaI 2956 TCATGA T^CATGA
SexAI 2417 ACCAGGT A^CC[AT]GGT
SnaBI 2696 TACGTA TAC^GTA
Van91I 2524 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3655 CCACTTATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 867 GTAGAC GT^[AC][GT]AC
1619 GTCTAC
Asp700I 1115 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1699 GAACTATTTC
AvaI 2896 CTCGGG C^[CT]CG[GA]G
3505 CCCGAG
BamHI 37 GGATCC G^GATCC
1079 GGATCC
BanI 913 GGCACC G^G[CT][GA]CC
3501 GGTACC
BanII 616 GGGCTC G[GA]GC[CT]^C
3826 GAGCCC
BglII 380 AGATCT A^GATCT
1397 AGATCT
BmyI 616 GGGCTC G[AGT]GC[ACT]^C
3826 GAGCCC
BsgI 1925 CTGCAC GTGCAG(16/14)
3709 GTGCAG
BsoBI 2896 CTCGGG C^[CT]CG[GA]G
3505 CCCGAG
Bsp1286I 616 GGGCTC G[AGT]GC[ACT]^C
3826 GAGCCC
Bsp1407I 2153 TGTACA T^GTACA
4220 TGTACA
BsrGI 2153 TGTACA T^GTACA
4220 TGTACA
BstAPI 647 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2514 GCATTAAATGC
HaeII 94 AGCGCC [GA]GCGC^[CT]
4000 GGCGCT
HindIII 1917 AAGCTT A^AGCTT
3892 AAGCTT
HpaI 3845 GTTAAC GTT^AAC
4295 GTTAAC
NspI 940 ACATGC [GA]CATG^[CT]
1854 GCATGT
ScaI 3453 AGTACT AGT^ACT
3785 AGTACT
SspBI 2153 TGTACA T^GTACA
4220 TGTACA
StuI 1741 AGGCCT AGG^CCT
2142 AGGCCT
XmnI 1115 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1699 GAACTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaII
BbvI BciVI BclI BcnI BfaI
BlpI BpmI Bpu1102I BsaAI BsaJI
BsiYI BslI BsmFI BsmI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DsaI EarI EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII