##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AocI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BbrPI
BbvCI BclI BfrI BglII BlnI
BsaBI BsiEI BsiWI BspLU11I BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CpoI EagI EclXI Eco52I Eco81I
EcoRV EcoT22I FbaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NcoI NdeI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SbfI ScaI SfuI SgrAI SnaBI
Sse8387I SspI SwaI Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 608 TCCGGA T^CCGGA
AcsI 60 GAATTC [GA]^AATT[CT]
AhdI 759 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 60 GAATTC [GA]^AATT[CT]
AspEI 759 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2152 GGATCC G^GATCC
BciVI 1756 GTATCC GTATCC(6/5)
BlpI 468 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 468 GCTGAGC GC^T[ACGT]AGC
BsaAI 369 TACGTG [CT]AC^GT[GA]
BsaWI 608 TCCGGA [AT]^CCGG[AT]
BseAI 608 TCCGGA T^CCGGA
BsmBI 1377 CGTCTC CGTCTC(1/5)
BsmI 2082 GAATGC GAATGC(1/-1)
Bsp1407I 3750 TGTACA T^GTACA
BspEI 608 TCCGGA T^CCGGA
BspHI 1909 TCATGA T^CATGA
BsrGI 3750 TGTACA T^GTACA
BstEII 3245 GGTCACC G^GT[ACGT]ACC
BstPI 3245 GGTCACC G^GT[ACGT]ACC
CelII 468 GCTGAGC GC^T[ACGT]AGC
DraI 3771 TTTAAA TTT^AAA
DraIII 1491 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3068 CCACGG C^C[GA][CT]GG
Eam1105I 759 GACTTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3245 GGTCACC G^GT[ACGT]ACC
EcoRI 60 GAATTC G^AATTC
FseI 3203 GGCCGGCC GGCCGG^CC
MroI 608 TCCGGA T^CCGGA
PaeR7I 262 CTCGAG C^TCGAG
RcaI 1909 TCATGA T^CATGA
SalI 3320 GTCGAC G^TCGAC
SfiI 2484 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 362 ACTAGT A^CTAGT
SphI 2221 GCATGC GCATG^C
SspBI 3750 TGTACA T^GTACA
StuI 3106 AGGCCT AGG^CCT
XhoI 262 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Aor51HI 1561 AGCGCT AGC^GCT
1933 AGCGCT
BbeI 1024 GGCGCC GGCGC^C
3481 GGCGCC
BsaI 1161 GAGACC GGTCTC(1/5)
1218 GAGACC
BspDI 462 ATCGAT AT^CGAT
2127 ATCGAT
BssSI 2226 CACGAG CACGAG(-5/-1)
35 CTCGTG
BstYI 2152 GGATCC [GA]^GATC[CT]
2572 AGATCC
ClaI 462 ATCGAT AT^CGAT
2127 ATCGAT
Eco47III 1561 AGCGCT AGC^GCT
1933 AGCGCT
HincII 234 GTCAAC GT[CT]^[GA]AC
3320 GTCGAC
HindII 234 GTCAAC GT[CT]^[GA]AC
3320 GTCGAC
KasI 1024 GGCGCC G^GCGCC
3481 GGCGCC
MflI 2152 GGATCC [GA]^GATC[CT]
2572 AGATCC
NarI 1024 GGCGCC GG^CGCC
3481 GGCGCC
NheI 566 GCTAGC G^CTAGC
3431 GCTAGC
PflMI 947 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1655 CCACGAACTGG
SexAI 2144 ACCAGGT A^CC[AT]GGT
2596 ACCTGGT
SfoI 1024 GGCGCC GGC^GCC
3481 GGCGCC
SmaI 2863 CCCGGG CCC^GGG
3013 CCCGGG
Tsp509I 61 AATT ^AATT
3021 AATT
Van91I 947 CCATACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1655 CCACGAACTGG
XcmI 877 CCAAGAACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1126 CCATGTATAACCTGG
XhoII 2152 GGATCC [GA]^GATC[CT]
2572 AGATCC
XmaCI 2863 CCCGGG C^CCGGG
3013 CCCGGG
XmaI 2863 CCCGGG C^CCGGG
3013 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII AviII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I