##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BpuAI BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BstEII BstPI BstUI BstXI Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MaeII MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI Tth111I
Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 285 GTATAC GT^[AC][GT]AC
Alw44I 861 GTGCAC G^TGCAC
AlwNI 1348 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 861 GTGCAC G^TGCAC
BbvCI 1528 CCTCAGC CCTCAGC(-5/-2)
BglI 15 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1158 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1158 GCTAAGC GC^T[ACGT]AGC
BsaBI 255 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 451 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 980 TCCGGT [AT]^CCGG[AT]
BsgI 433 GTGCAG GTGCAG(16/14)
BsmAI 217 GAGAC GTCTC(1/5)
BsmFI 1106 GGGAC GGGAC(10/14)
BspHI 1588 TCATGA T^CATGA
BsrDI 1747 GCAATG GCAATG(2/0)
Bst1107I 285 GTATAC GTA^TAC
BstAPI 426 GCAGCTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 337 TTCGAA TT^CGAA
BstZ17I 285 GTATAC GTA^TAC
CelII 1158 GCTAAGC GC^T[ACGT]AGC
Cfr13I 784 GGCCC G^G[ACGT]CC
DraII 783 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoNI 1353 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 783 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 558 GATATC GAT^ATC
EcoT14I 451 CCAAGG C^C[AT][AT]GG
FauI 1742 GCGGG CCCGC(4/6)
HindIII 1453 AAGCTT A^AGCTT
HphI 747 GGTGA GGTGA(8/7)
MamI 255 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 337 TTCGAA TT^CGAA
PleI 1585 GAGTC GAGTC(4/5)
RcaI 1588 TCATGA T^CATGA
SapI 2015 GAAGAGC GCTCTTC(1/4)
Sau96I 784 GGCCC G^G[ACGT]CC
SexAI 1630 ACCTGGT A^CC[AT]GGT
SfuI 337 TTCGAA TT^CGAA
SmlI 1197 CTTGAG C^T[CT][GA]AG
SspI 312 AATATT AAT^ATT
StyI 451 CCAAGG C^C[AT][AT]GG
SwaI 364 ATTTAAAT ATTT^AAAT
Tsp45I 1928 GTCAC ^GT[GC]AC
XbaI 1334 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 153 GTAC GT^AC
870 GTAC
Asp700I 1035 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2120 GAAACATTTC
AspHI 861 GTGCAC G[AT]GC[AT]^C
1066 GAGCAC
BalI 173 TGGCCA TGG^CCA
764 TGGCCA
BmyI 861 GTGCAC G[AGT]GC[ACT]^C
1066 GAGCAC
BpmI 928 CTCCAG CTGGAG(16/14)
1366 CTCCAG
BseRI 190 CTCCTC GAGGAG(10/8)
1810 GAGGAG
BsiHKAI 861 GTGCAC G[AT]GC[AT]^C
1066 GAGCAC
Bsp1286I 861 GTGCAC G[AGT]GC[ACT]^C
1066 GAGCAC
BssKI 329 CCAGG ^CC[ACGT]GG
1631 CCTGG
BssSI 1582 CACGAG CACGAG(-5/-1)
6 CTCGTG
BstF5I 1274 GGATG GGATG(2/0)
1470 GGATG
BstNI 329 CCAGG CC^[AT]GG
1631 CCTGG
BstYI 260 AGATCC [GA]^GATC[CT]
332 GGATCT
DraI 365 TTTAAA TTT^AAA
884 TTTAAA
EcoRII 329 CCAGG ^CC[AT]GG
1631 CCTGG
FokI 1274 GGATG GGATG(9/13)
1470 GGATG
HapII 981 CCGG C^CGG
1536 CCGG
HpaII 981 CCGG C^CGG
1536 CCGG
MflI 260 AGATCC [GA]^GATC[CT]
332 GGATCT
MluNI 173 TGGCCA TGG^CCA
764 TGGCCA
MscI 173 TGGCCA TGG^CCA
764 TGGCCA
MspA1I 931 CAGCTG C[AC]G^C[GT]G
1171 CAGCTG
MspI 981 CCGG C^CGG
1536 CCGG
MvaI 329 CCAGG CC^[AT]GG
1631 CCTGG
NlaIII 221 CATG CATG^
1589 CATG
NlaIV 12 GGAGCC GG[ACGT]^[ACGT]CC
1128 GGAGCC
PstI 280 CTGCAG CTGCA^G
1886 CTGCAG
PvuII 931 CAGCTG CAG^CTG
1171 CAGCTG
RsaI 153 GTAC GT^AC
870 GTAC
ScrFI 329 CCAGG CC^[ACGT]GG
1631 CCTGG
XcmI 1558 CCACTAAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1792 CCATTGTTTTTGTGG
XhoII 260 AGATCC [GA]^GATC[CT]
332 GGATCT
XmnI 1035 GAATCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2120 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BfaI BsiYI BslI BsrI
Cac8I DdeI DpnII EaeI EarI
Fnu4HI HaeIII HincII HindII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MwoI
NdeII Sau3AI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I