##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccII AflII AgeI
Alw44I ApaLI AscI Asp718I AspI
AviII AvrII BamHI BbeI BcgI
BclI BfrI BlnI BsiWI BsmBI
BspDI BspHI BspLU11I BsrDI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoNI FbaI FseI FspI
KasI KpnI KspI MluI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SmaI SnaBI
SpeI Sse8387I Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 2576 GTAGAC GT^[AC][GT]AC
AccIII 360 TCCGGA T^CCGGA
AclI 3900 AACGTT AA^CGTT
AcyI 580 GACGCC G[GA]^CG[CT]C
AflIII 11 ACACGT A^C[GA][CT]GT
AhdI 659 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1781 CCTGAGG CC^T[ACGT]AGG
Aor51HI 101 AGCGCT AGC^GCT
AseI 2041 ATTAAT AT^TAAT
AsnI 2041 ATTAAT AT^TAAT
AspEI 659 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 162 TGGCCA TGG^CCA
BbrPI 12 CACGTG CAC^GTG
BglII 481 AGATCT A^GATCT
BsaAI 12 CACGTG [CT]AC^GT[GA]
BsaHI 580 GACGCC G[GA]^CG[CT]C
BsaI 1102 GAGACC GGTCTC(1/5)
BseAI 360 TCCGGA T^CCGGA
BsiEI 1975 CGATCG CG[GA][CT]^CG
BsmI 685 GCATTC GAATGC(1/-1)
Bsp1407I 2459 TGTACA T^GTACA
BspEI 360 TCCGGA T^CCGGA
BsrFI 2018 GCCGGT [GA]^CCGG[CT]
BsrGI 2459 TGTACA T^GTACA
BstAPI 3760 GCATATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1331 GGTGACC G^GT[ACGT]ACC
BstPI 1331 GGTGACC G^GT[ACGT]ACC
Bsu36I 1781 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2018 GCCGGT [GA]^CCGG[CT]
DraIII 12 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 659 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 101 AGCGCT AGC^GCT
Eco81I 1781 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1331 GGTGACC G^GT[ACGT]ACC
EcoRI 2956 GAATTC G^AATTC
EcoRV 278 GATATC GAT^ATC
Hin1I 580 GACGCC G[GA]^CG[CT]C
MluNI 162 TGGCCA TGG^CCA
MroI 360 TCCGGA T^CCGGA
MscI 162 TGGCCA TGG^CCA
NdeI 3761 CATATG CA^TATG
PflMI 991 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 12 CACGTG CAC^GTG
PmlI 12 CACGTG CAC^GTG
PshBI 2041 ATTAAT AT^TAAT
Psp1406I 3900 AACGTT AA^CGTT
PvuI 1975 CGATCG CGAT^CG
SapI 1646 GAAGAGC GCTCTTC(1/4)
ScaI 2129 AGTACT AGT^ACT
SgrAI 2017 CGCCGGTG C[GA]^CCGG[CT]G
SspBI 2459 TGTACA T^GTACA
StuI 1136 AGGCCT AGG^CCT
SwaI 2493 ATTTAAAT ATTT^AAAT
Van91I 991 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2769 CCATGATTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
ApaI 980 GGGCCC GGGCC^C
1690 GGGCCC
Asp700I 2031 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2668 GAATTTCTTC
AvaI 925 CTCGGG C^[CT]CG[GA]G
1185 CTCGGG
BanI 941 GGTGCC G^G[CT][GA]CC
2262 GGTGCC
BbvCI 85 GCTGAGG CCTCAGC(-5/-2)
1088 GCTGAGG
BglI 728 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2995 GCCCAGCAGGC
BlpI 971 GCTAAGC GC^T[ACGT]AGC
1980 GCTCAGC
Bpu1102I 971 GCTAAGC GC^T[ACGT]AGC
1980 GCTCAGC
BsaBI 320 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
777 GATTCATATC
BsaWI 360 TCCGGA [AT]^CCGG[AT]
1036 ACCGGA
BsoBI 925 CTCGGG C^[CT]CG[GA]G
1185 CTCGGG
BsrBI 1649 GAGCGG CCGCTC(-3/-3)
1729 GAGCGG
BstYI 35 GGATCT [GA]^GATC[CT]
481 AGATCT
CelII 971 GCTAAGC GC^T[ACGT]AGC
1980 GCTCAGC
FauI 207 CCCGC CCCGC(4/6)
1651 GCGGG
HaeII 101 AGCGCT [GA]GCGC^[CT]
1014 AGCGCC
HgaI 580 GACGC GACGC(5/10)
1429 GCGTC
HpaI 1745 GTTAAC GTT^AAC
3146 GTTAAC
MamI 320 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
777 GATTCATATC
MflI 35 GGATCT [GA]^GATC[CT]
481 AGATCT
PpuMI 1054 AGGACCC [GA]G^G[AT]CC[CT]
1465 GGGACCT
SphI 1710 GCATGC GCATG^C
2814 GCATGC
SspI 2202 AATATT AAT^ATT
2546 AATATT
XhoII 35 GGATCT [GA]^GATC[CT]
481 AGATCT
XmnI 2031 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2668 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MnlI MseI MslI
MspA1I MspI MunI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I