##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
AspEI       AvrII       BamHI       BbrPI       BcgI        
BciVI       BglI        BglII       BlnI        BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstUI       BstZ17I     
Cfr10I      ClaI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoT22I     FseI        
HindIII     KspI        MamI        MluI        MroI        
MvnI        NaeI        NcoI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PvuI        SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I        471  GGTACC               G^GTACC
AclI          872  AACGTT               AA^CGTT
AcyI         3051  GGCGCC               G[GA]^CG[CT]C
AflIII        362  ACGTGT               A^C[GA][CT]GT
AlwNI        2617  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2239  CCTGAGG              CC^T[ACGT]AGG
ApaI         2647  GGGCCC               GGGCC^C
Asp700I      3451  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       471  GGTACC               G^GTACC
AspI         2105  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2445  TGCGCA               TGC^GCA
BanII        2647  GGGCCC               G[GA]GC[CT]^C
BbeI         3051  GGCGCC               GGCGC^C
BbsI          155  GAAGAC               GAAGAC(2/6)
BbvCI        1787  GCTGAGG              CCTCAGC(-5/-2)
BclI         2857  TGATCA               T^GATCA
BlpI           27  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       27  GCTGAGC              GC^T[ACGT]AGC
BpuAI         155  GAAGAC               GAAGAC(2/6)
BsaHI        3051  GGCGCC               G[GA]^CG[CT]C
BspHI        2038  TCATGA               T^CATGA
BssSI        2968  CTCGTG               CACGAG(-5/-1)
Bsu36I       2239  CCTGAGG              CC^T[ACGT]AGG
CelII          27  GCTGAGC              GC^T[ACGT]AGC
CpoI          651  CGGACCG              CG^G[AT]CCG
DrdI         1950  GACTTTTAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2239  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1874  CCTCAAATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1115  GAATTC               G^AATTC
EcoRV        1196  GATATC               GAT^ATC
FauI          978  CCCGC                CCCGC(4/6)
FbaI         2857  TGATCA               T^GATCA
FspI         2445  TGCGCA               TGC^GCA
Hin1I        3051  GGCGCC               G[GA]^CG[CT]C
HpaI          526  GTTAAC               GTT^AAC
KasI         3051  GGCGCC               G^GCGCC
KpnI          471  GGTACC               GGTAC^C
MfeI         2390  CAATTG               C^AATTG
MunI         2390  CAATTG               C^AATTG
NarI         3051  GGCGCC               GG^CGCC
NdeI         3296  CATATG               CA^TATG
PflFI        2105  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        3891  GACAAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      872  AACGTT               AA^CGTT
RcaI         2038  TCATGA               T^CATGA
RsrII         651  CGGACCG              CG^G[AT]CCG
SfoI         3051  GGCGCC               GGC^GCC
SmaI          784  CCCGGG               CCC^GGG
StuI         1919  AGGCCT               AGG^CCT
Tth111I      2105  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1974  CCAAGAGGCTCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         784  CCCGGG               C^CCGGG
XmaI          784  CCCGGG               C^CCGGG
XmnI         3451  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflII         147  CTTAAG               C^TTAAG
             3043  CTTAAG              
AseI         2845  ATTAAT               AT^TAAT
             3205  ATTAAT              
AsnI         2845  ATTAAT               AT^TAAT
             3205  ATTAAT              
AvaI          784  CCCGGG               C^[CT]CG[GA]G
             1467  CCCGAG              
BanI          471  GGTACC               G^G[CT][GA]CC
             3051  GGCGCC              
BfrI          147  CTTAAG               C^TTAAG
             3043  CTTAAG              
BsmAI        1326  GAGAC                GTCTC(1/5)
             4069  GAGAC               
BsmI         3596  GAATGC               GAATGC(1/-1)
             3247  GCATTC              
BsoBI         784  CCCGGG               C^[CT]CG[GA]G
             1467  CCCGAG              
BspMI         603  ACCTGC               ACCTGC(4/8)
             1930  GCAGGT              
BsrDI         673  CATTGC               GCAATG(2/0)
             1272  CATTGC              
BstEII       1348  GGTGACC              G^GT[ACGT]ACC
             1364  GGTGACC             
BstPI        1348  GGTGACC              G^GT[ACGT]ACC
             1364  GGTGACC             
BstXI         451  CCACAACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1848  CCAAGGATGTGG        
BstYI        1862  GGATCT               [GA]^GATC[CT]
             2796  AGATCC              
DraIII        495  CACTGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2174  CACTCAGTG           
EcoO65I      1348  GGTGACC              G^GT[ACGT]ACC
             1364  GGTGACC             
HgaI         1281  GACGC                GACGC(5/10)
             2379  GCGTC               
HincII        526  GTTAAC               GT[CT]^[GA]AC
             1292  GTTGAC              
HindII        526  GTTAAC               GT[CT]^[GA]AC
             1292  GTTGAC              
MflI         1862  GGATCT               [GA]^GATC[CT]
             2796  AGATCC              
PleI          793  GAGTC                GAGTC(4/5)
             2637  GAGTC               
PpuMI        3637  AGGACCC              [GA]G^G[AT]CC[CT]
             3853  GGGTCCC             
PshBI        2845  ATTAAT               AT^TAAT
             3205  ATTAAT              
PstI         2363  CTGCAG               CTGCA^G
             3626  CTGCAG              
PvuII         777  CAGCTG               CAG^CTG
              878  CAGCTG              
SspI          406  AATATT               AAT^ATT
             1382  AATATT              
XhoII        1862  GGATCT               [GA]^GATC[CT]
             2796  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BalI        BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I