##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbrPI BciVI BfrI
BglII BlnI BsaBI BseAI BsiWI
BspDI BspEI BspLU11I BsrDI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraIII Eam1105I Eco47III Eco81I
EcoRI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 3578 GACGTC GACGT^C
AflIII 3735 ACACGT A^C[GA][CT]GT
AgeI 2534 ACCGGT A^CCGGT
Alw44I 1572 GTGCAC G^TGCAC
ApaLI 1572 GTGCAC G^TGCAC
Asp700I 512 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 439 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 344 TGGCCA TGG^CCA
BanI 2574 GGCGCC G^G[CT][GA]CC
BbeI 2574 GGCGCC GGCGC^C
BcgI 4018 CGAGCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 130 TGATCA T^GATCA
BglI 888 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1847 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1847 GCTGAGC GC^T[ACGT]AGC
BsaAI 1299 TACGTG [CT]AC^GT[GA]
BsaWI 2534 ACCGGT [AT]^CCGG[AT]
BspHI 34 TCATGA T^CATGA
BssSI 4016 CACGAG CACGAG(-5/-1)
BstAPI 2756 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1847 GCTGAGC GC^T[ACGT]AGC
DraI 111 TTTAAA TTT^AAA
DraII 1191 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 1152 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3239 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1191 AGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 130 TGATCA T^GATCA
HincII 2979 GTCAAC GT[CT]^[GA]AC
HindII 2979 GTCAAC GT[CT]^[GA]AC
KasI 2574 GGCGCC G^GCGCC
KspI 2566 CCGCGG CCGC^GG
MluNI 344 TGGCCA TGG^CCA
MscI 344 TGGCCA TGG^CCA
NaeI 1410 GCCGGC GCC^GGC
NarI 2574 GGCGCC GG^CGCC
NgoMIV 1410 GCCGGC G^CCGGC
NspI 2752 ACATGC [GA]CATG^[CT]
PaeR7I 3524 CTCGAG C^TCGAG
PflFI 439 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 824 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2534 ACCGGT A^CCGGT
RcaI 34 TCATGA T^CATGA
SacII 2566 CCGCGG CCGC^GG
SfoI 2574 GGCGCC GGC^GCC
SgrAI 2533 CACCGGTG C[GA]^CCGG[CT]G
SmaI 574 CCCGGG CCC^GGG
Tth111I 439 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 824 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4070 TCTAGA T^CTAGA
XhoI 3524 CTCGAG C^TCGAG
XmaCI 574 CCCGGG C^CCGGG
XmaI 574 CCCGGG C^CCGGG
XmnI 512 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 118 AAATTT [GA]^AATT[CT]
1234 AAATTC
ApoI 118 AAATTT [GA]^AATT[CT]
1234 AAATTC
BsaI 672 GAGACC GGTCTC(1/5)
3315 GAGACC
BsiEI 1999 CGGCCG CG[GA][CT]^CG
2689 CGGCCG
Bsp1407I 840 TGTACA T^GTACA
4045 TGTACA
BsrBI 2546 CCGCTC CCGCTC(-3/-3)
3249 GAGCGG
BsrGI 840 TGTACA T^GTACA
4045 TGTACA
BstYI 278 AGATCC [GA]^GATC[CT]
1477 AGATCC
EagI 1999 CGGCCG C^GGCCG
2689 CGGCCG
EclXI 1999 CGGCCG C^GGCCG
2689 CGGCCG
Eco52I 1999 CGGCCG C^GGCCG
2689 CGGCCG
EcoRV 258 GATATC GAT^ATC
2833 GATATC
EcoT22I 665 ATGCAT ATGCA^T
766 ATGCAT
MflI 278 AGATCC [GA]^GATC[CT]
1477 AGATCC
NcoI 1648 CCATGG C^CATGG
3217 CCATGG
NsiI 665 ATGCAT ATGCA^T
766 ATGCAT
PstI 289 CTGCAG CTGCA^G
3998 CTGCAG
SapI 3767 GAAGAGC GCTCTTC(1/4)
2548 GCTCTTC
SmlI 726 CTCAAG C^T[CT][GA]AG
3524 CTCGAG
SspBI 840 TGTACA T^GTACA
4045 TGTACA
XhoII 278 AGATCC [GA]^GATC[CT]
1477 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI