##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
EcoO65I FauI FseI FspI Hin1I
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 2211 ACATGT A^C[GA][CT]GT
Alw44I 42 GTGCAC G^TGCAC
Aor51HI 1007 AGCGCT AGC^GCT
ApaI 3153 GGGCCC GGGCC^C
ApaLI 42 GTGCAC G^TGCAC
AspI 4095 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 2492 CCCGG CC^[GC]GG
BglII 2418 AGATCT A^GATCT
BsaWI 267 ACCGGA [AT]^CCGG[AT]
BsgI 3878 GTGCAG GTGCAG(16/14)
BsiEI 3294 CGATCG CG[GA][CT]^CG
Bsp1407I 471 TGTACA T^GTACA
BspHI 3347 TCATGA T^CATGA
BspLU11I 2211 ACATGT A^CATGT
BsrGI 471 TGTACA T^GTACA
DraI 1375 TTTAAA TTT^AAA
Eco47III 1007 AGCGCT AGC^GCT
EcoNI 3907 CCTTTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3979 ATGCAT ATGCA^T
HaeII 1007 AGCGCT [GA]GCGC^[CT]
HgaI 255 GCGTC GACGC(5/10)
HincII 457 GTTGAC GT[CT]^[GA]AC
HindII 457 GTTGAC GT[CT]^[GA]AC
MaeII 22 ACGT A^CGT
NciI 2492 CCCGG CC^[GC]GG
NsiI 3979 ATGCAT ATGCA^T
PflFI 4095 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 3294 CGATCG CGAT^CG
RcaI 3347 TCATGA T^CATGA
SacI 1645 GAGCTC GAGCT^C
SapI 2521 GCTCTTC GCTCTTC(1/4)
SpeI 1735 ACTAGT A^CTAGT
SphI 3278 GCATGC GCATG^C
SspBI 471 TGTACA T^GTACA
Tth111I 4095 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1 CGCG CG^CG
39 CGCG
AciI 4043 CCGC CCGC(-3/-1)
2649 GCGG
AocI 3237 CCTGAGG CC^T[ACGT]AGG
4022 CCTGAGG
BamHI 787 GGATCC G^GATCC
2996 GGATCC
BanI 1156 GGCACC G^G[CT][GA]CC
1606 GGCACC
BciVI 327 GGATAC GTATCC(6/5)
2349 GGATAC
BclI 357 TGATCA T^GATCA
774 TGATCA
BsaI 1638 GAGACC GGTCTC(1/5)
3004 GAGACC
BsmI 3977 GAATGC GAATGC(1/-1)
1508 GCATTC
BstAPI 412 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
834 GCATTGTGTGC
BstUI 1 CGCG CG^CG
39 CGCG
Bsu36I 3237 CCTGAGG CC^T[ACGT]AGG
4022 CCTGAGG
Eco81I 3237 CCTGAGG CC^T[ACGT]AGG
4022 CCTGAGG
EcoRI 1381 GAATTC G^AATTC
2184 GAATTC
FbaI 357 TGATCA T^GATCA
774 TGATCA
HapII 268 CCGG C^CGG
2493 CCGG
HpaII 268 CCGG C^CGG
2493 CCGG
MspI 268 CCGG C^CGG
2493 CCGG
MvnI 1 CGCG CG^CG
39 CGCG
NcoI 1152 CCATGG C^CATGG
3149 CCATGG
PpuMI 1403 AGGACCT [GA]G^G[AT]CC[CT]
1808 AGGACCC
ScaI 2234 AGTACT AGT^ACT
2273 AGTACT
SspI 1980 AATATT AAT^ATT
2947 AATATT
TaqI 519 TCGA T^CGA
2526 TCGA
TthHB8I 519 TCGA T^CGA
2526 TCGA
XcmI 2204 CCAGTGGACATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3157 CCAGATCGGGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI