##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BcnI BglI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstZ17I CelII CfoI Cfr10I
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoNI EcoRV FauI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaII KasI MluI
MroI MspI NaeI NarI NciI
NgoMIV NotI PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2732 GGTACC G^GTACC
AflII 2060 CTTAAG C^TTAAG
AhdI 1209 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 848 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2403 CCTGAGG CC^T[ACGT]AGG
Asp718I 2732 GGTACC G^GTACC
AspEI 1209 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2117 GTGCTC G[AT]GC[AT]^C
AspI 694 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 372 GGATCC G^GATCC
BbsI 937 GAAGAC GAAGAC(2/6)
BfrI 2060 CTTAAG C^TTAAG
BglII 2283 AGATCT A^GATCT
BpuAI 937 GAAGAC GAAGAC(2/6)
BsaAI 2832 TACGTG [CT]AC^GT[GA]
BsiEI 1 CGACCG CG[GA][CT]^CG
BsiHKAI 2117 GTGCTC G[AT]GC[AT]^C
BspDI 811 ATCGAT AT^CGAT
BspHI 3449 TCATGA T^CATGA
BspMI 1107 ACCTGC ACCTGC(4/8)
BsrDI 3371 GCAATG GCAATG(2/0)
BstBI 567 TTCGAA TT^CGAA
Bsu36I 2403 CCTGAGG CC^T[ACGT]AGG
Cac8I 2725 GCTAGC GC[ACGT]^[ACGT]GC
ClaI 811 ATCGAT AT^CGAT
DraII 1207 GGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1209 GACCTTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2403 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1207 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1081 ATGCAT ATGCA^T
HgaI 3000 GCGTC GACGC(5/10)
KpnI 2732 GGTACC GGTAC^C
KspI 4 CCGCGG CCGC^GG
MfeI 1016 CAATTG C^AATTG
MspA1I 4 CCGCGG C[AC]G^C[GT]G
MunI 1016 CAATTG C^AATTG
MwoI 1074 GCCTAGTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 2725 GCTAGC G^CTAGC
NruI 1271 TCGCGA TCG^CGA
NsiI 1081 ATGCAT ATGCA^T
NspV 567 TTCGAA TT^CGAA
PflFI 694 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1173 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1207 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 3449 TCATGA T^CATGA
SacII 4 CCGCGG CCGC^GG
SapI 2119 GCTCTTC GCTCTTC(1/4)
ScaI 3156 AGTACT AGT^ACT
SfuI 567 TTCGAA TT^CGAA
SpeI 135 ACTAGT A^CTAGT
Tth111I 694 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1173 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3444 CCAACTCATGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 5 CGCG CG^CG
1272 CGCG
Asp700I 937 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1859 GAAAAGCTTC
BalI 621 TGGCCA TGG^CCA
2253 TGGCCA
BanI 2732 GGTACC G^G[CT][GA]CC
2976 GGTGCC
BanII 843 GAGCCC G[GA]GC[CT]^C
2810 GGGCTC
BciVI 139 GTATCC GTATCC(6/5)
2409 GTATCC
BclI 743 TGATCA T^GATCA
756 TGATCA
BpmI 193 CTCCAG CTGGAG(16/14)
2634 CTCCAG
BseRI 513 CTCCTC GAGGAG(10/8)
4240 GAGGAG
BspLU11I 1410 ACATGT A^CATGT
2672 ACATGT
BstEII 408 GGTGACC G^GT[ACGT]ACC
1048 GGTGACC
BstPI 408 GGTGACC G^GT[ACGT]ACC
1048 GGTGACC
BstUI 5 CGCG CG^CG
1272 CGCG
BstXI 986 CCATTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3098 CCATGCTGTTGG
BstYI 372 GGATCC [GA]^GATC[CT]
2283 AGATCT
EaeI 621 TGGCCA [CT]^GGCC[GA]
2253 TGGCCA
EcoO65I 408 GGTGACC G^GT[ACGT]ACC
1048 GGTGACC
EcoRI 977 GAATTC G^AATTC
3914 GAATTC
FbaI 743 TGATCA T^GATCA
756 TGATCA
MflI 372 GGATCC [GA]^GATC[CT]
2283 AGATCT
MluNI 621 TGGCCA TGG^CCA
2253 TGGCCA
MscI 621 TGGCCA TGG^CCA
2253 TGGCCA
MvnI 5 CGCG CG^CG
1272 CGCG
NcoI 303 CCATGG C^CATGG
1053 CCATGG
NdeI 916 CATATG CA^TATG
2587 CATATG
TfiI 802 GATTC G^A[AT]TC
1030 GAATC
XhoII 372 GGATCC [GA]^GATC[CT]
2283 AGATCT
XmnI 937 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1859 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BbvI BfaI BmyI BsaBI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HindIII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I