##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

Acc65I      AclI        AgeI        AscI        AseI        
AsnI        Asp718I     AviII       AvrII       BciVI       
BglII       BlnI        BsaBI       BsiWI       BsmI        
Bsp1407I    BspDI       BsrGI       BssHII      BstBI       
ClaI        CpoI        DrdI        EcoRI       EcoRV       
EcoT22I     FspI        HincII      HindII      HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        1162  GACGTC               GACGT^C
AcsI          496  AAATTC               [GA]^AATT[CT]
AflII        1993  CTTAAG               C^TTAAG
AflIII       4106  ACATGT               A^C[GA][CT]GT
AhdI          646  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2541  CCTCAGG              CC^T[ACGT]AGG
Aor51HI      1226  AGCGCT               AGC^GCT
ApoI          496  AAATTC               [GA]^AATT[CT]
AspEI         646  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1004  TGGCCA               TGG^CCA
BamHI         116  GGATCC               G^GATCC
BbeI         3846  GGCGCC               GGCGC^C
BbrPI        3951  CACGTG               CAC^GTG
BbvCI        2136  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2075  GCAACAAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          878  TGATCA               T^GATCA
BfrI         1993  CTTAAG               C^TTAAG
BlpI         2796  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2796  GCTGAGC              GC^T[ACGT]AGC
BsaI         2762  GGTCTC               GGTCTC(1/5)
BspHI        3756  TCATGA               T^CATGA
BspLU11I     4106  ACATGT               A^CATGT
Bst1107I     1074  GTATAC               GTA^TAC
BstAPI       2292  GCAGTCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        3266  CCAGTGAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1074  GTATAC               GTA^TAC
Bsu36I       2541  CCTCAGG              CC^T[ACGT]AGG
CelII        2796  GCTGAGC              GC^T[ACGT]AGC
DraI         3186  TTTAAA               TTT^AAA
Eam1105I      646  GACAAAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1226  AGCGCT               AGC^GCT
Eco81I       2541  CCTCAGG              CC^T[ACGT]AGG
FbaI          878  TGATCA               T^GATCA
FseI         2501  GGCCGGCC             GGCCGG^CC
HindIII      3196  AAGCTT               A^AGCTT
KasI         3846  GGCGCC               G^GCGCC
MluNI        1004  TGGCCA               TGG^CCA
MscI         1004  TGGCCA               TGG^CCA
NarI         3846  GGCGCC               GG^CGCC
PflMI         996  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        3951  CACGTG               CAC^GTG
PmlI         3951  CACGTG               CAC^GTG
RcaI         3756  TCATGA               T^CATGA
SbfI         1908  CCTGCAGG             CCTGCA^GG
SfoI         3846  GGCGCC               GGC^GCC
Sse8387I     1908  CCTGCAGG             CCTGCA^GG
Van91I        996  CCACCCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AspI          705  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4127  GACTCGGTC           
BsmBI         745  GAGACG               CGTCTC(1/5)
             2224  GAGACG              
BsrDI        1455  CATTGC               GCAATG(2/0)
              257  GCAATG              
DraIII        526  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3875  CACCTGGTG           
EagI            6  CGGCCG               C^GGCCG
               34  CGGCCG              
EclXI           6  CGGCCG               C^GGCCG
               34  CGGCCG              
Eco52I          6  CGGCCG               C^GGCCG
               34  CGGCCG              
EcoNI        1242  CCTGTCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2492  CCTGAGCGAGG         
NaeI          615  GCCGGC               GCC^GGC
             2502  GCCGGC              
NcoI          641  CCATGG               C^CATGG
             3027  CCATGG              
NgoMIV        615  GCCGGC               G^CCGGC
             2502  GCCGGC              
PflFI         705  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4127  GACTCGGTC           
PshAI        2691  GACTCACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3383  GACCTTGGTC          
PvuII        1273  CAGCTG               CAG^CTG
             3094  CAGCTG              
ScaI          431  AGTACT               AGT^ACT
             1112  AGTACT              
TfiI           66  GATTC                G^A[AT]TC
              262  GATTC               
Tsp509I       497  AATT                 ^AATT
             3180  AATT                
Tth111I       705  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4127  GACTCGGTC           
XcmI          509  CCAGCTCGAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3867  CCAGTCGGCACCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcyI        
AfaI        AluI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       Asp700I     AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaAI       BsaHI       BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspEI       BspMI       BsrBI       BsrFI       
BsrI        BssKI       BssSI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MroI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     XhoII       
XmnI