##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp718I AviII BcgI BfrI
BsaBI BsiWI BspDI BssHII ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI ScaI SfiI SgrAI SnaBI
SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 751 GTATAC GT^[AC][GT]AC
AccIII 1822 TCCGGA T^CCGGA
AhdI 503 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 503 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1408 CCTAGG C^CTAGG
BamHI 2420 GGATCC G^GATCC
BclI 329 TGATCA T^GATCA
BglI 3993 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3838 AGATCT A^GATCT
BlnI 1408 CCTAGG C^CTAGG
BseAI 1822 TCCGGA T^CCGGA
BsiEI 558 CGACCG CG[GA][CT]^CG
Bsp1407I 3848 TGTACA T^GTACA
BspEI 1822 TCCGGA T^CCGGA
BspLU11I 435 ACATGT A^CATGT
BsrDI 646 CATTGC GCAATG(2/0)
BsrFI 1221 ACCGGC [GA]^CCGG[CT]
BsrGI 3848 TGTACA T^GTACA
BssSI 1097 CACGAG CACGAG(-5/-1)
Bst1107I 751 GTATAC GTA^TAC
BstZ17I 751 GTATAC GTA^TAC
Cfr10I 1221 ACCGGC [GA]^CCGG[CT]
Eam1105I 503 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1000 GAAGAG CTCTTC(1/4)
EcoNI 2494 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 89 ATGCAT ATGCA^T
FbaI 329 TGATCA T^GATCA
Ksp632I 1000 GAAGAG CTCTTC(1/4)
MroI 1822 TCCGGA T^CCGGA
NcoI 3071 CCATGG C^CATGG
NsiI 89 ATGCAT ATGCA^T
PaeR7I 2670 CTCGAG C^TCGAG
SacI 4027 GAGCTC GAGCT^C
SbfI 637 CCTGCAGG CCTGCA^GG
SmlI 2670 CTCGAG C^T[CT][GA]AG
Sse8387I 637 CCTGCAGG CCTGCA^GG
SspBI 3848 TGTACA T^GTACA
XhoI 2670 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Alw44I 3738 GTGCAC G^TGCAC
3790 GTGCAC
ApaLI 3738 GTGCAC G^TGCAC
3790 GTGCAC
Asp700I 1126 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3627 GAAAAAGTTC
AspI 1724 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2272 GACTCCGTC
BalI 3055 TGGCCA TGG^CCA
3074 TGGCCA
BbeI 1051 GGCGCC GGCGC^C
1754 GGCGCC
BciVI 176 GTATCC GTATCC(6/5)
2504 GTATCC
BsaWI 1822 TCCGGA [AT]^CCGG[AT]
4160 TCCGGT
BsmBI 3290 CGTCTC CGTCTC(1/5)
1304 GAGACG
BsmI 534 GAATGC GAATGC(1/-1)
4248 GCATTC
BspHI 1170 TCATGA T^CATGA
2406 TCATGA
BsrBI 3716 CCGCTC CCGCTC(-3/-3)
3768 CCGCTC
BstBI 282 TTCGAA TT^CGAA
1589 TTCGAA
DraI 1555 TTTAAA TTT^AAA
2200 TTTAAA
DraIII 152 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
2558 CACACCGTG
KasI 1051 GGCGCC G^GCGCC
1754 GGCGCC
MluNI 3055 TGGCCA TGG^CCA
3074 TGGCCA
MscI 3055 TGGCCA TGG^CCA
3074 TGGCCA
NarI 1051 GGCGCC GG^CGCC
1754 GGCGCC
NspV 282 TTCGAA TT^CGAA
1589 TTCGAA
PflFI 1724 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2272 GACTCCGTC
PpuMI 1677 AGGACCC [GA]G^G[AT]CC[CT]
2638 AGGACCT
PshAI 143 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1705 GACCAACGTC
RcaI 1170 TCATGA T^CATGA
2406 TCATGA
SexAI 2249 ACCAGGT A^CC[AT]GGT
4198 ACCAGGT
SfoI 1051 GGCGCC GGC^GCC
1754 GGCGCC
SfuI 282 TTCGAA TT^CGAA
1589 TTCGAA
SphI 627 GCATGC GCATG^C
3816 GCATGC
SspI 324 AATATT AAT^ATT
3856 AATATT
Tth111I 1724 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2272 GACTCCGTC
XmnI 1126 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3627 GAAAAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
Eco81I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI