##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AgeI        AhdI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbeI        
BcgI        BciVI       BclI        BfrI        BlnI        
BsaHI       BsiWI       BsmBI       BsmI        Bsp1407I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Cfr10I      DraI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       Hin1I       HpaI        KasI        KpnI        
KspI        MfeI        MluI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        SacII       SapI        SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        SwaI        TfiI        
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AccI         1337  GTCGAC               GT^[AC][GT]AC
AccII         986  CGCG                 CG^CG
AccIII       1250  TCCGGA               T^CCGGA
AfaI         2283  GTAC                 GT^AC
Alw44I        649  GTGCAC               G^TGCAC
AlwNI         743  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         649  GTGCAC               G^TGCAC
AspI         1291  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          466  GGCACC               G^G[CT][GA]CC
BbrPI         955  CACGTG               CAC^GTG
BbsI          501  GAAGAC               GAAGAC(2/6)
BglII         764  AGATCT               A^GATCT
BlpI         1156  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1156  GCTGAGC              GC^T[ACGT]AGC
BpuAI         501  GAAGAC               GAAGAC(2/6)
BsaBI        1005  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1030  GAGACC               GGTCTC(1/5)
BsaWI        1250  TCCGGA               [AT]^CCGG[AT]
BseAI        1250  TCCGGA               T^CCGGA
BsiEI         561  CGGTCG               CG[GA][CT]^CG
BspDI        1675  ATCGAT               AT^CGAT
BspEI        1250  TCCGGA               T^CCGGA
BspHI         725  TCATGA               T^CATGA
BspLU11I     2326  ACATGT               A^CATGT
BsrDI        1746  CATTGC               GCAATG(2/0)
BstUI         986  CGCG                 CG^CG
BstXI        1704  CCAGTGGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1156  GCTGAGC              GC^T[ACGT]AGC
ClaI         1675  ATCGAT               AT^CGAT
CpoI         1252  CGGACCG              CG^G[AT]CCG
EcoNI         582  CCTGGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      2238  CCTTGG               C^C[AT][AT]GG
HgaI          417  GACGC                GACGC(5/10)
MamI         1005  GATTTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1250  TCCGGA               T^CCGGA
MseI         1904  TTAA                 T^TAA
MslI            2  CACTGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          986  CGCG                 CG^CG
NspI         2326  ACATGT               [GA]CATG^[CT]
PflFI        1291  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         955  CACGTG               CAC^GTG
PmlI          955  CACGTG               CAC^GTG
RcaI          725  TCATGA               T^CATGA
RsaI         2283  GTAC                 GT^AC
RsrII        1252  CGGACCG              CG^G[AT]CCG
SacI         2092  GAGCTC               GAGCT^C
SalI         1337  GTCGAC               G^TCGAC
SbfI          549  CCTGCAGG             CCTGCA^GG
ScaI         2282  AGTACT               AGT^ACT
SexAI         674  ACCAGGT              A^CC[AT]GGT
Sse8387I      549  CCTGCAGG             CCTGCA^GG
StuI         1602  AGGCCT               AGG^CCT
StyI         2238  CCTTGG               C^C[AT][AT]GG
Tru9I        1904  TTAA                 T^TAA
Tth111I      1291  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflIII       2156  ACACGT               A^C[GA][CT]GT
             2326  ACATGT              
AocI         1018  CCTCAGG              CC^T[ACGT]AGG
             2127  CCTGAGG             
Asp700I       156  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              180  GAAAGCTTTC          
AvaI          626  CCCGGG               C^[CT]CG[GA]G
             1479  CCCGGG              
BalI          659  TGGCCA               TGG^CCA
             1697  TGGCCA              
BanII        1987  GGGCTC               G[GA]GC[CT]^C
             2092  GAGCTC              
BbvCI        1350  CCTCAGC              CCTCAGC(-5/-2)
              532  GCTGAGG             
BglI          798  GCCTGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1771  GCCAGCTTGGC         
BpmI         2335  CTCCAG               CTGGAG(16/14)
              808  CTGGAG              
BsaAI         955  CACGTG               [CT]AC^GT[GA]
             2157  CACGTA              
BsoBI         626  CCCGGG               C^[CT]CG[GA]G
             1479  CCCGGG              
BspMI         552  GCAGGT               ACCTGC(4/8)
             1212  GCAGGT              
Bsu36I       1018  CCTCAGG              CC^T[ACGT]AGG
             2127  CCTGAGG             
CfoI          792  GCGC                 GCG^C
              879  GCGC                
DrdI         1240  GACATTGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2155  GACACGTATGTC        
EarI         2396  CTCTTC               CTCTTC(1/4)
              496  GAAGAG              
Eco81I       1018  CCTCAGG              CC^T[ACGT]AGG
             2127  CCTGAGG             
HhaI          792  GCGC                 GCG^C
              879  GCGC                
HinP1I        792  GCGC                 G^CGC
              879  GCGC                
HindIII       182  AAGCTT               A^AGCTT
             2292  AAGCTT              
Ksp632I      2396  CTCTTC               CTCTTC(1/4)
              496  GAAGAG              
MaeII         956  ACGT                 A^CGT
             2158  ACGT                
MluNI         659  TGGCCA               TGG^CCA
             1697  TGGCCA              
MscI          659  TGGCCA               TGG^CCA
             1697  TGGCCA              
SmaI          626  CCCGGG               CCC^GGG
             1479  CCCGGG              
Tsp509I      2025  AATT                 ^AATT
             2193  AATT                
XcmI         1685  CCATGTTGGAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1743  CCACATTGCAGATGG     
XmaCI         626  CCCGGG               C^CCGGG
             1479  CCCGGG              
XmaI          626  CCCGGG               C^CCGGG
             1479  CCCGGG              
XmnI          156  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              180  GAAAGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BbvI        BcnI        BfaI        BmyI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DraIII      
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PstI        PvuII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII