##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII ApaI AscI Asp718I AviII
AvrII BamHI BbeI BbvCI BfrI
BlnI BsaBI BsaHI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraIII EagI EclXI
Eco52I EcoRI FseI FspI Hin1I
KasI KpnI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NotI NruI NspV PaeR7I PmeI
PshAI Psp1406I PvuI RsrII SacII
SapI SbfI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AhdI 2857 GACGCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3863 GTGCAC G^TGCAC
AocI 2230 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1242 AGCGCT AGC^GCT
ApaLI 3863 GTGCAC G^TGCAC
AseI 3257 ATTAAT AT^TAAT
AsnI 3257 ATTAAT AT^TAAT
AspEI 2857 GACGCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 935 CACGTG CAC^GTG
BcgI 1813 CGAATCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1117 GGATAC GTATCC(6/5)
BlpI 2005 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2005 GCTGAGC GC^T[ACGT]AGC
BsgI 639 GTGCAG GTGCAG(16/14)
BsmBI 1465 CGTCTC CGTCTC(1/5)
BspHI 861 TCATGA T^CATGA
BsrBI 1613 CCGCTC CCGCTC(-3/-3)
BsrDI 284 CATTGC GCAATG(2/0)
BstAPI 543 GCAAATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2230 CCTGAGG CC^T[ACGT]AGG
CelII 2005 GCTGAGC GC^T[ACGT]AGC
Eam1105I 2857 GACGCTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1242 AGCGCT AGC^GCT
Eco81I 2230 CCTGAGG CC^T[ACGT]AGG
EcoNI 2990 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 334 ATGCAT ATGCA^T
HaeII 1242 AGCGCT [GA]GCGC^[CT]
HindIII 2048 AAGCTT A^AGCTT
HpaI 2167 GTTAAC GTT^AAC
MfeI 2199 CAATTG C^AATTG
MunI 2199 CAATTG C^AATTG
NheI 308 GCTAGC G^CTAGC
NsiI 334 ATGCAT ATGCA^T
PacI 731 TTAATTAA TTAAT^TAA
PmaCI 935 CACGTG CAC^GTG
PmlI 935 CACGTG CAC^GTG
PshBI 3257 ATTAAT AT^TAAT
PstI 2796 CTGCAG CTGCA^G
RcaI 861 TCATGA T^CATGA
SacI 1001 GAGCTC GAGCT^C
SalI 593 GTCGAC G^TCGAC
ScaI 3661 AGTACT AGT^ACT
SfcI 2796 CTGCAG C^T[GA][CT]AG
SmaI 704 CCCGGG CCC^GGG
SspI 2490 AATATT AAT^ATT
StuI 2811 AGGCCT AGG^CCT
XmaCI 704 CCCGGG C^CCGGG
XmaI 704 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AgeI 2613 ACCGGT A^CCGGT
3001 ACCGGT
AlwNI 1222 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
2799 CAGAGGCTG
AspI 1854 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2786 GACTGCGTC
BbsI 1639 GAAGAC GAAGAC(2/6)
1937 GAAGAC
BglII 3420 AGATCT A^GATCT
3693 AGATCT
BpuAI 1639 GAAGAC GAAGAC(2/6)
1937 GAAGAC
BsiEI 1858 CGGTCG CG[GA][CT]^CG
2694 CGGTCG
BspMI 19 GCAGGT ACCTGC(4/8)
3589 GCAGGT
BstEII 1444 GGTGACC G^GT[ACGT]ACC
2539 GGTGACC
BstPI 1444 GGTGACC G^GT[ACGT]ACC
2539 GGTGACC
CfoI 17 GCGC GCG^C
1243 GCGC
DraI 3329 TTTAAA TTT^AAA
3889 TTTAAA
DrdI 1447 GACCAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2818 GACAGGGAAGTC
EcoO65I 1444 GGTGACC G^GT[ACGT]ACC
2539 GGTGACC
EcoRV 1776 GATATC GAT^ATC
3294 GATATC
HhaI 17 GCGC GCG^C
1243 GCGC
HinP1I 17 GCGC G^CGC
1243 GCGC
NspI 589 GCATGT [GA]CATG^[CT]
597 ACATGC
PflFI 1854 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2786 GACTGCGTC
PflMI 2107 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2392 CCAGCCCATGG
PinAI 2613 ACCGGT A^CCGGT
3001 ACCGGT
SfiI 1837 GGCCATCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3541 GGCCTCTTGGGCC
SmlI 982 CTCAAG C^T[CT][GA]AG
1664 CTCAAG
Tth111I 1854 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2786 GACTGCGTC
Van91I 2107 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2392 CCAGCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbvI BclI BcnI BfaI
BglI BmyI BpmI BsaAI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI