##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AclI        AscI        AseI        AsnI        
Asp718I     AspI        AvaI        AvrII       BamHI       
BanI        BbeI        BbrPI       BciVI       BglII       
BlnI        BsaAI       BsaBI       BsiWI       BsoBI       
BspDI       BssHII      Bst1107I    BstBI       BstZ17I     
ClaI        DraI        DrdI        EcoT22I     FseI        
KasI        KpnI        MamI        MfeI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        SalI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SwaI        Tth111I     
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AatII         522  GACGTC               GACGT^C
AccIII       4033  TCCGGA               T^CCGGA
AgeI         2830  ACCGGT               A^CCGGT
Alw44I       5947  GTGCAC               G^TGCAC
Aor51HI      1936  AGCGCT               AGC^GCT
ApaLI        5947  GTGCAC               G^TGCAC
AviII        3538  TGCGCA               TGC^GCA
BbvCI        2154  GCTGAGG              CCTCAGC(-5/-2)
BclI         3412  TGATCA               T^GATCA
BseAI        4033  TCCGGA               T^CCGGA
Bsp1407I      329  TGTACA               T^GTACA
BspEI        4033  TCCGGA               T^CCGGA
BsrGI         329  TGTACA               T^GTACA
BstEII       1495  GGTTACC              G^GT[ACGT]ACC
BstPI        1495  GGTTACC              G^GT[ACGT]ACC
CpoI         5442  CGGTCCG              CG^G[AT]CCG
DraIII        642  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         5447  CGGCCG               C^GGCCG
EclXI        5447  CGGCCG               C^GGCCG
Eco47III     1936  AGCGCT               AGC^GCT
Eco52I       5447  CGGCCG               C^GGCCG
EcoO65I      1495  GGTTACC              G^GT[ACGT]ACC
EcoRV        2475  GATATC               GAT^ATC
FbaI         3412  TGATCA               T^GATCA
FspI         3538  TGCGCA               TGC^GCA
HpaI         4452  GTTAAC               GTT^AAC
KspI         5450  CCGCGG               CCGC^GG
MluI         2495  ACGCGT               A^CGCGT
MroI         4033  TCCGGA               T^CCGGA
PinAI        2830  ACCGGT               A^CCGGT
PshAI        4107  GACCTCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        5442  CGGTCCG              CG^G[AT]CCG
SacII        5450  CCGCGG               CCGC^GG
SbfI         4796  CCTGCAGG             CCTGCA^GG
SphI         2212  GCATGC               GCATG^C
Sse8387I     4796  CCTGCAGG             CCTGCA^GG
SspBI         329  TGTACA               T^GTACA
XbaI         1802  TCTAGA               T^CTAGA
XcmI         2209  CCAGCATGCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         2976  GTCTAC               GT^[AC][GT]AC
             5967  GTAGAC              
AcyI          522  GACGTC               G[GA]^CG[CT]C
             3111  GACGCC              
AflII        1425  CTTAAG               C^TTAAG
             2546  CTTAAG              
AhdI         4119  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5365  GACCCTCTGTC         
AocI         2927  CCTGAGG              CC^T[ACGT]AGG
             5278  CCTCAGG             
ApaI         1888  GGGCCC               GGGCC^C
             5929  GGGCCC              
Asp700I        81  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1956  GAAAAGCTTC          
AspEI        4119  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5365  GACCCTCTGTC         
BfrI         1425  CTTAAG               C^TTAAG
             2546  CTTAAG              
BglI         2163  GCCTTGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4438  GCCTTCCTGGC         
BsaHI         522  GACGTC               G[GA]^CG[CT]C
             3111  GACGCC              
BsaWI        2830  ACCGGT               [AT]^CCGG[AT]
             4033  TCCGGA              
BsmBI         524  CGTCTC               CGTCTC(1/5)
             3225  GAGACG              
BspLU11I     5080  ACATGT               A^CATGT
             5769  ACATGT              
BsrBI        3965  CCGCTC               CCGCTC(-3/-3)
             4041  GAGCGG              
BsrFI        2830  ACCGGT               [GA]^CCGG[CT]
             3169  ACCGGC              
BstAPI        248  GCAAATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1763  GCAGATTTTGC         
Bsu36I       2927  CCTGAGG              CC^T[ACGT]AGG
             5278  CCTCAGG             
Cfr10I       2830  ACCGGT               [GA]^CCGG[CT]
             3169  ACCGGC              
Eam1105I     4119  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5365  GACCCTCTGTC         
Eco81I       2927  CCTGAGG              CC^T[ACGT]AGG
             5278  CCTCAGG             
Hin1I         522  GACGTC               G[GA]^CG[CT]C
             3111  GACGCC              
SspI           45  AATATT               AAT^ATT
             3636  AATATT              
StuI         1333  AGGCCT               AGG^CCT
             3688  AGGCCT              
XmnI           81  GAATACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1956  GAAAAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaII       BalI        BanII       BbsI        BbvI        
BcgI        BcnI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspHI       BspMI       BsrDI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RcaI        RsaI        SacI        SapI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII