##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbvCI BciVI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BspEI BssHII BstUI BstXI Bsu36I
CelII CfoI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI FauI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacII
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
BalI 3163 TGGCCA TGG^CCA
BcgI 2309 GCAGTTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 47 CCCGG CC^[GC]GG
BglI 678 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2435 AGATCT A^GATCT
BsiEI 965 CGATCG CG[GA][CT]^CG
BsmBI 1831 CGTCTC CGTCTC(1/5)
BspDI 963 ATCGAT AT^CGAT
BsrBI 2926 CCGCTC CCGCTC(-3/-3)
BsrFI 3588 GCCGGT [GA]^CCGG[CT]
BssSI 3247 CTCGTG CACGAG(-5/-1)
BstAPI 2469 GCATTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2317 TTCGAA TT^CGAA
BstEII 4038 GGTTACC G^GT[ACGT]ACC
BstPI 4038 GGTTACC G^GT[ACGT]ACC
Cfr10I 3588 GCCGGT [GA]^CCGG[CT]
ClaI 963 ATCGAT AT^CGAT
DraII 167 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1165 CCGTGG C^C[GA][CT]GG
EaeI 3163 TGGCCA [CT]^GGCC[GA]
EcoNI 159 CCTTTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 167 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 4038 GGTTACC G^GT[ACGT]ACC
MaeII 777 ACGT A^CGT
MluNI 3163 TGGCCA TGG^CCA
MscI 3163 TGGCCA TGG^CCA
NciI 47 CCCGG CC^[GC]GG
NdeI 258 CATATG CA^TATG
NspV 2317 TTCGAA TT^CGAA
PaeR7I 3319 CTCGAG C^TCGAG
PpuMI 167 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 965 CGATCG CGAT^CG
SalI 3099 GTCGAC G^TCGAC
SfuI 2317 TTCGAA TT^CGAA
SphI 3686 GCATGC GCATG^C
StuI 2851 AGGCCT AGG^CCT
XcmI 1307 CCAGATGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3319 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 1441 ACATGT A^C[GA][CT]GT
3944 ACATGT
AseI 2471 ATTAAT AT^TAAT
4135 ATTAAT
AsnI 2471 ATTAAT AT^TAAT
4135 ATTAAT
Asp700I 3378 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4125 GAACGGCTTC
AvaI 1672 CTCGGG C^[CT]CG[GA]G
3319 CTCGAG
BamHI 3418 GGATCC G^GATCC
3893 GGATCC
BbsI 1593 GAAGAC GAAGAC(2/6)
2832 GAAGAC
BclI 3173 TGATCA T^GATCA
3872 TGATCA
BpuAI 1593 GAAGAC GAAGAC(2/6)
2832 GAAGAC
BsaI 358 GAGACC GGTCTC(1/5)
1759 GGTCTC
BsoBI 1672 CTCGGG C^[CT]CG[GA]G
3319 CTCGAG
BspHI 2264 TCATGA T^CATGA
3386 TCATGA
BspLU11I 1441 ACATGT A^CATGT
3944 ACATGT
BspMI 1053 GCAGGT ACCTGC(4/8)
3782 GCAGGT
Bst1107I 1840 GTATAC GTA^TAC
3940 GTATAC
BstZ17I 1840 GTATAC GTA^TAC
3940 GTATAC
EcoRV 2544 GATATC GAT^ATC
3630 GATATC
EcoT14I 3052 CCAAGG C^C[AT][AT]GG
3713 CCTTGG
FbaI 3173 TGATCA T^GATCA
3872 TGATCA
HapII 48 CCGG C^CGG
3589 CCGG
HgaI 1118 GACGC GACGC(5/10)
2210 GCGTC
HindIII 2003 AAGCTT A^AGCTT
3522 AAGCTT
HpaII 48 CCGG C^CGG
3589 CCGG
MslI 1050 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1901 CACAAATATG
MspI 48 CCGG C^CGG
3589 CCGG
PshBI 2471 ATTAAT AT^TAAT
4135 ATTAAT
PstI 2250 CTGCAG CTGCA^G
2770 CTGCAG
RcaI 2264 TCATGA T^CATGA
3386 TCATGA
SexAI 170 ACCTGGT A^CC[AT]GGT
2957 ACCTGGT
SspI 828 AATATT AAT^ATT
2021 AATATT
StyI 3052 CCAAGG C^C[AT][AT]GG
3713 CCTTGG
XbaI 822 TCTAGA T^CTAGA
2123 TCTAGA
XmnI 3378 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4125 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII