##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AscI AspI AvrII
BcgI BfrI BglII BlnI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI MluI MroI NdeI
NotI NruI NsiI NspV PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SalI SfiI SfuI SgrAI
SnaBI SspBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AhdI 3865 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1803 GTGCAC G^TGCAC
AocI 352 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2181 AGCGCT AGC^GCT
ApaLI 1803 GTGCAC G^TGCAC
AseI 4208 ATTAAT AT^TAAT
AsnI 4208 ATTAAT AT^TAAT
Asp700I 4249 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3865 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 972 GGATCC G^GATCC
BbrPI 698 CACGTG CAC^GTG
BciVI 3095 GGATAC GTATCC(6/5)
BclI 2163 TGATCA T^GATCA
BsaAI 698 CACGTG [CT]AC^GT[GA]
BsaWI 2467 ACCGGA [AT]^CCGG[AT]
BsiEI 89 CGGCCG CG[GA][CT]^CG
BsmBI 317 GAGACG CGTCTC(1/5)
BspHI 895 TCATGA T^CATGA
BsrDI 1626 GCAATG GCAATG(2/0)
BssHII 52 GCGCGC G^CGCGC
Bsu36I 352 CCTGAGG CC^T[ACGT]AGG
DraIII 2992 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2709 GACCATCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 89 CGGCCG C^GGCCG
Eam1105I 3865 GACTTCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 89 CGGCCG C^GGCCG
Eco47III 2181 AGCGCT AGC^GCT
Eco52I 89 CGGCCG C^GGCCG
Eco81I 352 CCTGAGG CC^T[ACGT]AGG
FbaI 2163 TGATCA T^GATCA
HincII 2661 GTCAAC GT[CT]^[GA]AC
HindII 2661 GTCAAC GT[CT]^[GA]AC
KspI 937 CCGCGG CCGC^GG
MfeI 4055 CAATTG C^AATTG
MunI 4055 CAATTG C^AATTG
NaeI 1478 GCCGGC GCC^GGC
NgoMIV 1478 GCCGGC G^CCGGC
PacI 4386 TTAATTAA TTAAT^TAA
PaeR7I 1647 CTCGAG C^TCGAG
PflMI 2140 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 698 CACGTG CAC^GTG
PmlI 698 CACGTG CAC^GTG
PshBI 4208 ATTAAT AT^TAAT
RcaI 895 TCATGA T^CATGA
SacII 937 CCGCGG CCGC^GG
SapI 3068 GCTCTTC GCTCTTC(1/4)
ScaI 582 AGTACT AGT^ACT
SexAI 2993 ACCAGGT A^CC[AT]GGT
SpeI 3690 ACTAGT A^CTAGT
SphI 958 GCATGC GCATG^C
Van91I 2140 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2704 CCATTGACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1647 CTCGAG C^TCGAG
XmnI 4249 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 927 GGTACC G^GTACC
3594 GGTACC
AcsI 3109 GAATTT [GA]^AATT[CT]
4075 AAATTC
ApoI 3109 GAATTT [GA]^AATT[CT]
4075 AAATTC
Asp718I 927 GGTACC G^GTACC
3594 GGTACC
BalI 1497 TGGCCA TGG^CCA
2092 TGGCCA
BsaBI 1428 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2569 GATACTGATC
BsgI 272 CTGCAC GTGCAG(16/14)
2491 GTGCAG
BsmI 1198 GAATGC GAATGC(1/-1)
4361 GCATTC
BsrBI 1223 GAGCGG CCGCTC(-3/-3)
1697 GAGCGG
BstXI 3187 CCATTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3754 CCAGGAGGCTGG
KpnI 927 GGTACC GGTAC^C
3594 GGTACC
MamI 1428 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2569 GATACTGATC
MluNI 1497 TGGCCA TGG^CCA
2092 TGGCCA
MscI 1497 TGGCCA TGG^CCA
2092 TGGCCA
MslI 3188 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3830 CACCCACATG
NheI 331 GCTAGC G^CTAGC
3847 GCTAGC
NspI 616 ACATGC [GA]CATG^[CT]
958 GCATGC
SbfI 976 CCTGCAGG CCTGCA^GG
3259 CCTGCAGG
SmaI 646 CCCGGG CCC^GGG
753 CCCGGG
Sse8387I 976 CCTGCAGG CCTGCA^GG
3259 CCTGCAGG
XmaCI 646 CCCGGG C^CCGGG
753 CCCGGG
XmaI 646 CCCGGG C^CCGGG
753 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AviII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII