##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI Asp718I AvrII BbrPI BcgI
BciVI BglI BlnI BsaAI BsaBI
BsmBI Bsp1407I BspDI BsrBI BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoO65I
FseI HgaI KpnI KspI MamI
MfeI MluI MunI NaeI NgoMIV
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 802 TCCGGA T^CCGGA
AflIII 3051 ACATGT A^C[GA][CT]GT
AhdI 2748 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 141 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 448 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2748 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1649 CCCGAG C^[CT]CG[GA]G
AviII 15 TGCGCA TGC^GCA
BamHI 3077 GGATCC G^GATCC
BlpI 2048 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2048 GCTGAGC GC^T[ACGT]AGC
BsaI 2617 GGTCTC GGTCTC(1/5)
BsaWI 802 TCCGGA [AT]^CCGG[AT]
BseAI 802 TCCGGA T^CCGGA
BsiEI 2647 CGGTCG CG[GA][CT]^CG
BsiWI 2326 CGTACG C^GTACG
BsoBI 1649 CCCGAG C^[CT]CG[GA]G
BspEI 802 TCCGGA T^CCGGA
BspLU11I 3051 ACATGT A^CATGT
BssHII 135 GCGCGC G^CGCGC
BstAPI 2589 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 2048 GCTGAGC GC^T[ACGT]AGC
DraI 3712 TTTAAA TTT^AAA
DrdI 1385 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2748 GACATAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 15 TGCGCA TGC^GCA
HpaI 1729 GTTAAC GTT^AAC
MroI 802 TCCGGA T^CCGGA
NdeI 981 CATATG CA^TATG
NheI 1463 GCTAGC G^CTAGC
PflMI 780 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 525 AGTACT AGT^ACT
SexAI 3057 ACCAGGT A^CC[AT]GGT
Van91I 780 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 156 CCATCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 448 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1291 GTCTAC GT^[AC][GT]AC
1630 GTCTAC
AcyI 1872 GGCGCC G[GA]^CG[CT]C
1930 GGCGCC
AflII 1525 CTTAAG C^TTAAG
2453 CTTAAG
Alw44I 1081 GTGCAC G^TGCAC
3406 GTGCAC
ApaI 2478 GGGCCC GGGCC^C
2962 GGGCCC
ApaLI 1081 GTGCAC G^TGCAC
3406 GTGCAC
AspI 1370 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2026 GACGAAGTC
BbeI 1872 GGCGCC GGCGC^C
1930 GGCGCC
BbvCI 1423 GCTGAGG CCTCAGC(-5/-2)
2972 GCTGAGG
BclI 969 TGATCA T^GATCA
1189 TGATCA
BfrI 1525 CTTAAG C^TTAAG
2453 CTTAAG
BglII 1901 AGATCT A^GATCT
2367 AGATCT
BsaHI 1872 GGCGCC G[GA]^CG[CT]C
1930 GGCGCC
BsmI 2420 GAATGC GAATGC(1/-1)
3303 GAATGC
BspHI 966 TCATGA T^CATGA
2362 TCATGA
BsrDI 2139 GCAATG GCAATG(2/0)
3089 GCAATG
DraIII 33 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
2088 CACACTGTG
EcoNI 1296 CCTCTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2278 CCTTGAAGAGG
EcoRI 962 GAATTC G^AATTC
3193 GAATTC
FauI 132 CCCGC CCCGC(4/6)
290 GCGGG
FbaI 969 TGATCA T^GATCA
1189 TGATCA
HaeII 1872 GGCGCC [GA]GCGC^[CT]
1930 GGCGCC
Hin1I 1872 GGCGCC G[GA]^CG[CT]C
1930 GGCGCC
HindIII 1273 AAGCTT A^AGCTT
1569 AAGCTT
KasI 1872 GGCGCC G^GCGCC
1930 GGCGCC
MaeII 716 ACGT A^CGT
2766 ACGT
NarI 1872 GGCGCC GG^CGCC
1930 GGCGCC
NcoI 225 CCATGG C^CATGG
1762 CCATGG
PflFI 1370 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2026 GACGAAGTC
RcaI 966 TCATGA T^CATGA
2362 TCATGA
SfoI 1872 GGCGCC GGC^GCC
1930 GGCGCC
StuI 1304 AGGCCT AGG^CCT
1889 AGGCCT
Tth111I 1370 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2026 GACGAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AocI ApoI AseI AsnI
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoRV EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII