##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI Aor51HI AseI AsnI
AspEI AspI AviII BciVI BfrI
BsaBI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HindIII HpaI MamI MfeI
MluI MroI MslI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfuI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1060 GTCTAC GT^[AC][GT]AC
Alw44I 3170 GTGCAC G^TGCAC
ApaLI 3170 GTGCAC G^TGCAC
AscI 2366 GGCGCGCC GG^CGCGCC
Asp700I 120 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 3198 TGGCCA TGG^CCA
BbeI 2266 GGCGCC GGCGC^C
BbrPI 2200 CACGTG CAC^GTG
BbsI 601 GTCTTC GAAGAC(2/6)
BcgI 2768 GCATGAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 601 GTCTTC GAAGAC(2/6)
BsaAI 2200 CACGTG [CT]AC^GT[GA]
BsmI 2761 GAATGC GAATGC(1/-1)
BspLU11I 953 ACATGT A^CATGT
BssSI 2776 CTCGTG CACGAG(-5/-1)
BstAPI 1347 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2907 CCATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 781 GACGGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 120 GAATTC G^AATTC
KasI 2266 GGCGCC G^GCGCC
MluNI 3198 TGGCCA TGG^CCA
MscI 3198 TGGCCA TGG^CCA
MseI 2999 TTAA T^TAA
NarI 2266 GGCGCC GG^CGCC
PmaCI 2200 CACGTG CAC^GTG
PmlI 2200 CACGTG CAC^GTG
SapI 2876 GCTCTTC GCTCTTC(1/4)
SbfI 3265 CCTGCAGG CCTGCA^GG
SexAI 607 ACCTGGT A^CC[AT]GGT
SfiI 2289 GGCCGAAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2266 GGCGCC GGC^GCC
SgrAI 2048 CGCCGGTG C[GA]^CCGG[CT]G
SphI 3407 GCATGC GCATG^C
Sse8387I 3265 CCTGCAGG CCTGCA^GG
Tru9I 2999 TTAA T^TAA
XmnI 120 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 120 GAATTC [GA]^AATT[CT]
1725 GAATTT
AcyI 1046 GACGCC G[GA]^CG[CT]C
2266 GGCGCC
ApoI 120 GAATTC [GA]^AATT[CT]
1725 GAATTT
AvrII 811 CCTAGG C^CTAGG
3205 CCTAGG
BclI 1082 TGATCA T^GATCA
1716 TGATCA
BlnI 811 CCTAGG C^CTAGG
3205 CCTAGG
BlpI 686 GCTGAGC GC^T[ACGT]AGC
1576 GCTCAGC
Bpu1102I 686 GCTGAGC GC^T[ACGT]AGC
1576 GCTCAGC
BsaHI 1046 GACGCC G[GA]^CG[CT]C
2266 GGCGCC
BsmBI 1315 GAGACG CGTCTC(1/5)
2578 GAGACG
CelII 686 GCTGAGC GC^T[ACGT]AGC
1576 GCTCAGC
DraIII 1502 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2197 CACCACGTG
FbaI 1082 TGATCA T^GATCA
1716 TGATCA
Hin1I 1046 GACGCC G[GA]^CG[CT]C
2266 GGCGCC
HincII 581 GTCAAC GT[CT]^[GA]AC
2829 GTCAAC
HindII 581 GTCAAC GT[CT]^[GA]AC
2829 GTCAAC
KspI 831 CCGCGG CCGC^GG
2362 CCGCGG
NcoI 672 CCATGG C^CATGG
848 CCATGG
SacII 831 CCGCGG CCGC^GG
2362 CCGCGG
SmlI 1473 CTTGAG C^T[CT][GA]AG
1803 CTTGAG
Tsp509I 121 AATT ^AATT
1726 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
Acc65I AccII AciI AfaI AflIII
AluI AlwI AlwNI ApaI Asp718I
AspHI AvaI AvaII BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BglII BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI