##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII BamHI
BcgI BciVI BclI BfrI BglII
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
KpnI KspI MamI MluI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 3365 TCCGGA T^CCGGA
AflIII 3613 ACACGT A^C[GA][CT]GT
Aor51HI 1299 AGCGCT AGC^GCT
Asp700I 1349 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 134 CCTAGG C^CTAGG
BbrPI 803 CACGTG CAC^GTG
BlnI 134 CCTAGG C^CTAGG
BsaI 1652 GAGACC GGTCTC(1/5)
BseAI 3365 TCCGGA T^CCGGA
BsiEI 580 CGACCG CG[GA][CT]^CG
BsmBI 1037 GAGACG CGTCTC(1/5)
BsmI 3606 GAATGC GAATGC(1/-1)
BspEI 3365 TCCGGA T^CCGGA
BsrDI 1333 CATTGC GCAATG(2/0)
DrdI 415 GACACGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 460 CCGTGG C^C[GA][CT]GG
Eco47III 1299 AGCGCT AGC^GCT
HincII 229 GTTAAC GT[CT]^[GA]AC
HindII 229 GTTAAC GT[CT]^[GA]AC
HindIII 113 AAGCTT A^AGCTT
HpaI 229 GTTAAC GTT^AAC
MfeI 3890 CAATTG C^AATTG
MroI 3365 TCCGGA T^CCGGA
MslI 1570 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 3890 CAATTG C^AATTG
NheI 346 GCTAGC G^CTAGC
NspI 3599 ACATGC [GA]CATG^[CT]
PflMI 3555 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 803 CACGTG CAC^GTG
PmlI 803 CACGTG CAC^GTG
SapI 3815 GAAGAGC GCTCTTC(1/4)
Van91I 3555 CCAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1349 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 421 GACGTC GACGT^C
1066 GACGTC
AhdI 1061 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1157 GACCGACTGTC
AspEI 1061 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1157 GACCGACTGTC
BbsI 3035 GAAGAC GAAGAC(2/6)
3320 GAAGAC
BpuAI 3035 GAAGAC GAAGAC(2/6)
3320 GAAGAC
BsaAI 803 CACGTG [CT]AC^GT[GA]
3614 CACGTA
BsaWI 3136 ACCGGA [AT]^CCGG[AT]
3365 TCCGGA
BsgI 71 CTGCAC GTGCAG(16/14)
1112 GTGCAG
BsrBI 679 CCGCTC CCGCTC(-3/-3)
3818 GAGCGG
BssSI 200 CACGAG CACGAG(-5/-1)
409 CTCGTG
BstAPI 925 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3663 GCATTTATTGC
BstYI 996 AGATCC [GA]^GATC[CT]
2721 AGATCC
Eam1105I 1061 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1157 GACCGACTGTC
MflI 996 AGATCC [GA]^GATC[CT]
2721 AGATCC
MseI 230 TTAA T^TAA
3744 TTAA
PaeR7I 1 CTCGAG C^TCGAG
788 CTCGAG
PpuMI 1422 AGGACCT [GA]G^G[AT]CC[CT]
2606 AGGTCCC
TfiI 1084 GATTC G^A[AT]TC
2942 GAATC
Tru9I 230 TTAA T^TAA
3744 TTAA
XhoI 1 CTCGAG C^TCGAG
788 CTCGAG
XhoII 996 AGATCC [GA]^GATC[CT]
2721 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI