##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AflII AgeI Aor51HI
AscI AseI AsnI Asp718I AspI
BbrPI BcgI BciVI BfrI BglII
BsaAI BsaBI BsiEI BsiWI BssHII
BstAPI CpoI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI HindIII
HpaI KpnI MamI MfeI MluI
MunI NdeI NheI NotI NruI
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SfiI SgrAI SnaBI SspI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 2546 TCCGGA T^CCGGA
AhdI 1809 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4345 GTGCAC G^TGCAC
ApaLI 4345 GTGCAC G^TGCAC
AspEI 1809 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1588 TGCGCA TGC^GCA
BclI 3148 TGATCA T^GATCA
BglI 2603 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 2546 TCCGGA T^CCGGA
Bsp1407I 4270 TGTACA T^GTACA
BspEI 2546 TCCGGA T^CCGGA
BspHI 3257 TCATGA T^CATGA
BspMI 3949 GCAGGT ACCTGC(4/8)
BsrGI 4270 TGTACA T^GTACA
BssSI 1434 CTCGTG CACGAG(-5/-1)
Bst1107I 3243 GTATAC GTA^TAC
BstBI 2395 TTCGAA TT^CGAA
BstEII 3318 GGTGACC G^GT[ACGT]ACC
BstPI 3318 GGTGACC G^GT[ACGT]ACC
BstZ17I 3243 GTATAC GTA^TAC
DraIII 3313 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1809 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3318 GGTGACC G^GT[ACGT]ACC
EcoT22I 3193 ATGCAT ATGCA^T
FbaI 3148 TGATCA T^GATCA
FspI 1588 TGCGCA TGC^GCA
MroI 2546 TCCGGA T^CCGGA
NaeI 2699 GCCGGC GCC^GGC
NgoMIV 2699 GCCGGC G^CCGGC
NsiI 3193 ATGCAT ATGCA^T
NspV 2395 TTCGAA TT^CGAA
PaeR7I 2965 CTCGAG C^TCGAG
RcaI 3257 TCATGA T^CATGA
SacI 4249 GAGCTC GAGCT^C
SalI 2175 GTCGAC G^TCGAC
SapI 2951 GAAGAGC GCTCTTC(1/4)
SbfI 2116 CCTGCAGG CCTGCA^GG
SfuI 2395 TTCGAA TT^CGAA
SpeI 513 ACTAGT A^CTAGT
Sse8387I 2116 CCTGCAGG CCTGCA^GG
SspBI 4270 TGTACA T^GTACA
SwaI 383 ATTTAAAT ATTT^AAAT
TfiI 920 GATTC G^A[AT]TC
XhoI 2965 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1596 GACGTC GACGT^C
3074 GACGTC
AflIII 2030 ACATGT A^C[GA][CT]GT
4226 ACATGT
BalI 773 TGGCCA TGG^CCA
3112 TGGCCA
BbeI 155 GGCGCC GGCGC^C
1568 GGCGCC
BbsI 1545 GAAGAC GAAGAC(2/6)
3706 GAAGAC
BlpI 2367 GCTGAGC GC^T[ACGT]AGC
2792 GCTGAGC
Bpu1102I 2367 GCTGAGC GC^T[ACGT]AGC
2792 GCTGAGC
BpuAI 1545 GAAGAC GAAGAC(2/6)
3706 GAAGAC
BsaI 2567 GAGACC GGTCTC(1/5)
1816 GGTCTC
BsaWI 2212 ACCGGA [AT]^CCGG[AT]
2546 TCCGGA
BspDI 165 ATCGAT AT^CGAT
2778 ATCGAT
BspLU11I 2030 ACATGT A^CATGT
4226 ACATGT
BsrFI 324 ACCGGC [GA]^CCGG[CT]
2699 GCCGGC
CelII 2367 GCTGAGC GC^T[ACGT]AGC
2792 GCTGAGC
Cfr10I 324 ACCGGC [GA]^CCGG[CT]
2699 GCCGGC
ClaI 165 ATCGAT AT^CGAT
2778 ATCGAT
DraI 181 TTTAAA TTT^AAA
384 TTTAAA
DrdI 1502 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1548 GACAGCGTGGTC
EcoNI 2036 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2254 CCTACAGAAGG
HaeII 155 GGCGCC [GA]GCGC^[CT]
1568 GGCGCC
KasI 155 GGCGCC G^GCGCC
1568 GGCGCC
KspI 110 CCGCGG CCGC^GG
127 CCGCGG
MluNI 773 TGGCCA TGG^CCA
3112 TGGCCA
MscI 773 TGGCCA TGG^CCA
3112 TGGCCA
NarI 155 GGCGCC GG^CGCC
1568 GGCGCC
NcoI 3451 CCATGG C^CATGG
4080 CCATGG
SacII 110 CCGCGG CCGC^GG
127 CCGCGG
ScaI 516 AGTACT AGT^ACT
2482 AGTACT
SexAI 1045 ACCAGGT A^CC[AT]GGT
3314 ACCTGGT
SfoI 155 GGCGCC GGC^GCC
1568 GGCGCC
SphI 2797 GCATGC GCATG^C
3191 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI Asp700I AspHI AvaI
AvaII AvrII BamHI BanI BanII
BbvCI BbvI BcnI BfaI BlnI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI