##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AccIII      AcsI        AgeI        ApoI        AscI        
AvrII       BamHI       BbeI        BbvCI       BcgI        
BclI        BlnI        BseAI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BsrBI       BsrGI       
BssHII      Bst1107I    BstBI       BstXI       BstZ17I     
ClaI        CpoI        DrdI        EcoRI       EcoRV       
EcoT22I     FbaI        FseI        KasI        KspI        
MfeI        MroI        MunI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PaeR7I      PflMI       PinAI       PmeI        PshAI       
PvuI        RcaI        RsrII       SacII       SapI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SspBI       SwaI        
Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AatII        3067  GACGTC               GACGT^C
Acc65I       2352  GGTACC               G^GTACC
AclI         2617  AACGTT               AA^CGTT
AflII        2407  CTTAAG               C^TTAAG
AhdI           43  GACTTCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2717  AGCGCT               AGC^GCT
AseI         3839  ATTAAT               AT^TAAT
AsnI         3839  ATTAAT               AT^TAAT
Asp700I      3618  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2352  GGTACC               G^GTACC
AspEI          43  GACTTCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1878  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        2858  TGCGCA               TGC^GCA
BbrPI         661  CACGTG               CAC^GTG
BciVI        1713  GTATCC               GTATCC(6/5)
BfrI         2407  CTTAAG               C^TTAAG
BglI         2135  GCCACCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         661  CACGTG               [CT]AC^GT[GA]
BsaBI        3366  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          710  GCATTC               GAATGC(1/-1)
BsrDI        2051  GCAATG               GCAATG(2/0)
BstEII       2754  GGTGACC              G^GT[ACGT]ACC
BstPI        2754  GGTGACC              G^GT[ACGT]ACC
DraI         4003  TTTAAA               TTT^AAA
EagI         2554  CGGCCG               C^GGCCG
Eam1105I       43  GACTTCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2554  CGGCCG               C^GGCCG
Eco47III     2717  AGCGCT               AGC^GCT
Eco52I       2554  CGGCCG               C^GGCCG
EcoO65I      2754  GGTGACC              G^GT[ACGT]ACC
FspI         2858  TGCGCA               TGC^GCA
HindIII      3061  AAGCTT               A^AGCTT
HpaI         4090  GTTAAC               GTT^AAC
KpnI         2352  GGTACC               GGTAC^C
MamI         3366  GATCCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         2459  ACGCGT               A^CGCGT
PacI         4177  TTAATTAA             TTAAT^TAA
PflFI        1878  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         661  CACGTG               CAC^GTG
PmlI          661  CACGTG               CAC^GTG
PpuMI        3050  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI        3839  ATTAAT               AT^TAAT
Psp1406I     2617  AACGTT               AA^CGTT
SacI         2920  GAGCTC               GAGCT^C
SexAI        3053  ACCTGGT              A^CC[AT]GGT
StuI         2261  AGGCCT               AGG^CCT
Tth111I      1878  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3934  TCTAGA               T^CTAGA
XmnI         3618  GAAGTTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI          721  CCTGAGG              CC^T[ACGT]AGG
             2416  CCTGAGG             
BglII        1151  AGATCT               A^GATCT
             3590  AGATCT              
BlpI         2377  GCTGAGC              GC^T[ACGT]AGC
             2966  GCTCAGC             
Bpu1102I     2377  GCTGAGC              GC^T[ACGT]AGC
             2966  GCTCAGC             
BsaI         2182  GAGACC               GGTCTC(1/5)
             3265  GAGACC              
BsaWI        1470  TCCGGT               [AT]^CCGG[AT]
             4216  ACCGGA              
BspLU11I     1144  ACATGT               A^CATGT
             2696  ACATGT              
Bsu36I        721  CCTGAGG              CC^T[ACGT]AGG
             2416  CCTGAGG             
CelII        2377  GCTGAGC              GC^T[ACGT]AGC
             2966  GCTCAGC             
EarI          340  CTCTTC               CTCTTC(1/4)
              279  GAAGAG              
Eco81I        721  CCTGAGG              CC^T[ACGT]AGG
             2416  CCTGAGG             
EcoNI         198  CCTCGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1989  CCTGTGTCAGG         
Ksp632I       340  CTCTTC               CTCTTC(1/4)
              279  GAAGAG              
NaeI         3097  GCCGGC               GCC^GGC
             3172  GCCGGC              
NgoMIV       3097  GCCGGC               G^CCGGC
             3172  GCCGGC              
PvuII         682  CAGCTG               CAG^CTG
             3824  CAGCTG              
SalI          733  GTCGAC               G^TCGAC
             1884  GTCGAC              
SphI         2399  GCATGC               GCATG^C
             2894  GCATGC              
SspI         3836  AATATT               AAT^ATT
             4158  AATATT              
TfiI           10  GAATC                G^A[AT]TC
             2526  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  120 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AflIII      AluI        Alw44I      AlwI        AlwNI       
ApaI        ApaLI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PstI        RsaI        Sau3AI      Sau96I      SbfI        
ScrFI       SfaNI       SfcI        SmlI        Sse8387I    
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII