##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AvrII BbrPI BcgI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI FseI KspI
MamI MluI MroI NaeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I StuI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1271 GGTACC G^GTACC
AccI 770 GTCTAC GT^[AC][GT]AC
AccII 1276 CGCG CG^CG
AflIII 3141 ACATGT A^C[GA][CT]GT
Asp718I 1271 GGTACC G^GTACC
AspI 819 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3615 GGATCC G^GATCC
BanII 3022 GAGCTC G[GA]GC[CT]^C
BbeI 94 GGCGCC GGCGC^C
BlpI 2755 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2755 GCTGAGC GC^T[ACGT]AGC
BsaI 2428 GAGACC GGTCTC(1/5)
BsmI 2532 GCATTC GAATGC(1/-1)
Bsp1407I 4569 TGTACA T^GTACA
BspDI 2422 ATCGAT AT^CGAT
BspLU11I 3141 ACATGT A^CATGT
BsrBI 2507 CCGCTC CCGCTC(-3/-3)
BsrGI 4569 TGTACA T^GTACA
BstUI 1276 CGCG CG^CG
BstXI 4353 CCACTCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2755 GCTGAGC GC^T[ACGT]AGC
ClaI 2422 ATCGAT AT^CGAT
DrdI 3886 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2550 GATATC GAT^ATC
EcoT22I 3563 ATGCAT ATGCA^T
FauI 75 GCGGG CCCGC(4/6)
HaeII 94 GGCGCC [GA]GCGC^[CT]
HindIII 1778 AAGCTT A^AGCTT
HpaI 2923 GTTAAC GTT^AAC
KasI 94 GGCGCC G^GCGCC
KpnI 1271 GGTACC GGTAC^C
MfeI 4058 CAATTG C^AATTG
MunI 4058 CAATTG C^AATTG
MvnI 1276 CGCG CG^CG
NarI 94 GGCGCC GG^CGCC
NdeI 1167 CATATG CA^TATG
NsiI 3563 ATGCAT ATGCA^T
PaeR7I 275 CTCGAG C^TCGAG
PflFI 819 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3022 GAGCTC GAGCT^C
SfoI 94 GGCGCC GGC^GCC
SmaI 50 CCCGGG CCC^GGG
SpeI 4077 ACTAGT A^CTAGT
SphI 3036 GCATGC GCATG^C
SspBI 4569 TGTACA T^GTACA
SwaI 4554 ATTTAAAT ATTT^AAAT
Tth111I 819 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1147 TCTAGA T^CTAGA
XhoI 275 CTCGAG C^TCGAG
XmaCI 50 CCCGGG C^CCGGG
XmaI 50 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 1606 AACGTT AA^CGTT
1805 AACGTT
AflII 2038 CTTAAG C^TTAAG
4449 CTTAAG
AvaI 50 CCCGGG C^[CT]CG[GA]G
275 CTCGAG
AviII 2471 TGCGCA TGC^GCA
2520 TGCGCA
BalI 2417 TGGCCA TGG^CCA
3754 TGGCCA
BbvCI 87 CCTCAGC CCTCAGC(-5/-2)
2296 CCTCAGC
BclI 795 TGATCA T^GATCA
834 TGATCA
BfrI 2038 CTTAAG C^TTAAG
4449 CTTAAG
BglII 1562 AGATCT A^GATCT
3323 AGATCT
BsgI 2543 CTGCAC GTGCAG(16/14)
3665 GTGCAG
BsoBI 50 CCCGGG C^[CT]CG[GA]G
275 CTCGAG
BspHI 3503 TCATGA T^CATGA
3882 TCATGA
BstAPI 706 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2463 GCAGCAGATGC
EaeI 2417 TGGCCA [CT]^GGCC[GA]
3754 TGGCCA
FbaI 795 TGATCA T^GATCA
834 TGATCA
FspI 2471 TGCGCA TGC^GCA
2520 TGCGCA
HapII 51 CCGG C^CGG
596 CCGG
HpaII 51 CCGG C^CGG
596 CCGG
MluNI 2417 TGGCCA TGG^CCA
3754 TGGCCA
MscI 2417 TGGCCA TGG^CCA
3754 TGGCCA
MspI 51 CCGG C^CGG
596 CCGG
NcoI 1155 CCATGG C^CATGG
2455 CCATGG
Psp1406I 1606 AACGTT AA^CGTT
1805 AACGTT
RcaI 3503 TCATGA T^CATGA
3882 TCATGA
ScaI 552 AGTACT AGT^ACT
2651 AGTACT
SspI 2144 AATATT AAT^ATT
3129 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanI BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI