##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AviII AvrII BbeI BbrPI BglI
BlnI BsaHI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HgaI
Hin1I KasI KpnI KspI MluI
MroI NaeI NarI NgoMIV NotI
NruI NspV PacI PinAI PmaCI
PmlI PvuI RsrII SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccII 926 CGCG CG^CG
AflII 6320 CTTAAG C^TTAAG
ApaI 6405 GGGCCC GGGCC^C
AspI 266 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 5086 CCTCAGC CCTCAGC(-5/-2)
BcgI 342 GCAGGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 5026 TGATCA T^GATCA
BfrI 6320 CTTAAG C^TTAAG
BlpI 7873 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 7873 GCTAAGC GC^T[ACGT]AGC
BspHI 2897 TCATGA T^CATGA
BstAPI 516 GCAATGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 926 CGCG CG^CG
CelII 7873 GCTAAGC GC^T[ACGT]AGC
EcoNI 1130 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4859 GATATC GAT^ATC
EcoT22I 519 ATGCAT ATGCA^T
FbaI 5026 TGATCA T^GATCA
HaeII 2906 GGCGCT [GA]GCGC^[CT]
MvnI 926 CGCG CG^CG
NcoI 1912 CCATGG C^CATGG
NsiI 519 ATGCAT ATGCA^T
PflFI 266 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmeI 8363 GTTTAAAC GTTT^AAAC
PshAI 7108 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2897 TCATGA T^CATGA
SapI 2746 GAAGAGC GCTCTTC(1/4)
SbfI 7206 CCTGCAGG CCTGCA^GG
SnaBI 3694 TACGTA TAC^GTA
SphI 521 GCATGC GCATG^C
Sse8387I 7206 CCTGCAGG CCTGCA^GG
SwaI 1873 ATTTAAAT ATTT^AAAT
Tth111I 266 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 740 CCATAATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 590 ACATGT A^C[GA][CT]GT
788 ACATGT
Alw44I 4918 GTGCAC G^TGCAC
6844 GTGCAC
ApaLI 4918 GTGCAC G^TGCAC
6844 GTGCAC
BamHI 2450 GGATCC G^GATCC
5033 GGATCC
BcnI 5985 CCGGG CC^[GC]GG
6041 CCCGG
BglII 1517 AGATCT A^GATCT
7593 AGATCT
BsaAI 3694 TACGTA [CT]AC^GT[GA]
6949 CACGTA
BsaBI 5842 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7462 GATGAGGATC
BsaWI 720 TCCGGT [AT]^CCGG[AT]
4960 ACCGGA
BspLU11I 590 ACATGT A^CATGT
788 ACATGT
BssSI 2934 CACGAG CACGAG(-5/-1)
1888 CTCGTG
DraIII 5481 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
6328 CACAAAGTG
FauI 6735 CCCGC CCCGC(4/6)
6770 CCCGC
MamI 5842 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7462 GATGAGGATC
MfeI 5010 CAATTG C^AATTG
7850 CAATTG
MunI 5010 CAATTG C^AATTG
7850 CAATTG
NciI 5985 CCGGG CC^[GC]GG
6041 CCCGG
PaeR7I 1177 CTCGAG C^TCGAG
3163 CTCGAG
PflMI 2372 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2513 CCAGCCTCTGG
PpuMI 2552 AGGTCCC [GA]G^G[AT]CC[CT]
7307 AGGACCC
SacI 2202 GAGCTC GAGCT^C
3679 GAGCTC
StuI 5271 AGGCCT AGG^CCT
5439 AGGCCT
Van91I 2372 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2513 CCAGCCTCTGG
XhoI 1177 CTCGAG C^TCGAG
3163 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AclI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NheI NlaIII NlaIV NspI PleI
PshBI Psp1406I PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI