##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AflIII AhdI Aor51HI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII BbrPI BlpI Bpu1102I
BsiEI BsiWI BsoBI BspLU11I BsrBI
BssHII BssSI BstAPI BstBI CelII
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MluI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacII SbfI SexAI SfiI SfuI
SgrAI SmaI SpeI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccIII 1445 TCCGGA T^CCGGA
AclI 3616 AACGTT AA^CGTT
AflII 3901 CTTAAG C^TTAAG
AgeI 4348 ACCGGT A^CCGGT
Alw44I 3588 GTGCAC G^TGCAC
AocI 4056 CCTGAGG CC^T[ACGT]AGG
ApaI 3443 GGGCCC GGGCC^C
ApaLI 3588 GTGCAC G^TGCAC
AseI 4336 ATTAAT AT^TAAT
AsnI 4336 ATTAAT AT^TAAT
BalI 1317 TGGCCA TGG^CCA
BbeI 1631 GGCGCC GGCGC^C
BclI 4196 TGATCA T^GATCA
BfrI 3901 CTTAAG C^TTAAG
BglI 1032 GCCATTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3351 TACGTA [CT]AC^GT[GA]
BsaBI 1419 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1449 GAGACC GGTCTC(1/5)
BseAI 1445 TCCGGA T^CCGGA
Bsp1407I 4325 TGTACA T^GTACA
BspDI 3346 ATCGAT AT^CGAT
BspEI 1445 TCCGGA T^CCGGA
BspMI 926 GCAGGT ACCTGC(4/8)
BsrGI 4325 TGTACA T^GTACA
Bst1107I 621 GTATAC GTA^TAC
BstEII 717 GGTGACC G^GT[ACGT]ACC
BstPI 717 GGTGACC G^GT[ACGT]ACC
BstXI 155 CCAACTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 621 GTATAC GTA^TAC
Bsu36I 4056 CCTGAGG CC^T[ACGT]AGG
ClaI 3346 ATCGAT AT^CGAT
DraI 4403 TTTAAA TTT^AAA
DraIII 528 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1317 TGGCCA [CT]^GGCC[GA]
Eco81I 4056 CCTGAGG CC^T[ACGT]AGG
EcoNI 27 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 717 GGTGACC G^GT[ACGT]ACC
FauI 1 CCCGC CCCGC(4/6)
FbaI 4196 TGATCA T^GATCA
HaeII 1631 GGCGCC [GA]GCGC^[CT]
KasI 1631 GGCGCC G^GCGCC
MamI 1419 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2106 CAATTG C^AATTG
MluNI 1317 TGGCCA TGG^CCA
MroI 1445 TCCGGA T^CCGGA
MscI 1317 TGGCCA TGG^CCA
MslI 3182 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2106 CAATTG C^AATTG
NarI 1631 GGCGCC GG^CGCC
NcoI 1770 CCATGG C^CATGG
NdeI 2503 CATATG CA^TATG
PinAI 4348 ACCGGT A^CCGGT
PshBI 4336 ATTAAT AT^TAAT
Psp1406I 3616 AACGTT AA^CGTT
SalI 1387 GTCGAC G^TCGAC
SapI 2898 GAAGAGC GCTCTTC(1/4)
ScaI 182 AGTACT AGT^ACT
SfoI 1631 GGCGCC GGC^GCC
SnaBI 3351 TACGTA TAC^GTA
SphI 2008 GCATGC GCATG^C
SspBI 4325 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 17 CGCG CG^CG
3863 CGCG
AcyI 1631 GGCGCC G[GA]^CG[CT]C
4097 GACGCC
AlwNI 1906 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2965 CAGAAGCTG
BamHI 842 GGATCC G^GATCC
2621 GGATCC
BbvCI 3092 CCTCAGC CCTCAGC(-5/-2)
3653 CCTCAGC
BciVI 1774 GGATAC GTATCC(6/5)
790 GTATCC
BsaHI 1631 GGCGCC G[GA]^CG[CT]C
4097 GACGCC
BsaWI 1445 TCCGGA [AT]^CCGG[AT]
4348 ACCGGT
BsgI 2266 CTGCAC GTGCAG(16/14)
1704 GTGCAG
BsmBI 1804 CGTCTC CGTCTC(1/5)
4095 GAGACG
BsmI 904 GAATGC GAATGC(1/-1)
4087 GAATGC
BspHI 2434 TCATGA T^CATGA
2846 TCATGA
BsrFI 1741 ACCGGC [GA]^CCGG[CT]
4348 ACCGGT
BstUI 17 CGCG CG^CG
3863 CGCG
Cfr10I 1741 ACCGGC [GA]^CCGG[CT]
4348 ACCGGT
DrdI 67 GACAAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3869 GACCTCTGTGTC
EcoRI 2985 GAATTC G^AATTC
3983 GAATTC
HgaI 4097 GACGC GACGC(5/10)
1803 GCGTC
Hin1I 1631 GGCGCC G[GA]^CG[CT]C
4097 GACGCC
HincII 1387 GTCGAC GT[CT]^[GA]AC
3677 GTTGAC
HindII 1387 GTCGAC GT[CT]^[GA]AC
3677 GTTGAC
MvnI 17 CGCG CG^CG
3863 CGCG
PflMI 527 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4022 CCACTGCCTGG
PpuMI 150 AGGACCC [GA]G^G[AT]CC[CT]
3478 AGGACCT
PstI 204 CTGCAG CTGCA^G
3551 CTGCAG
RcaI 2434 TCATGA T^CATGA
2846 TCATGA
SmlI 1885 CTCAAG C^T[CT][GA]AG
3901 CTTAAG
StuI 1303 AGGCCT AGG^CCT
3745 AGGCCT
Van91I 527 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4022 CCACTGCCTGG
XbaI 1520 TCTAGA T^CTAGA
2049 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII AvrII
BanI BanII BbsI BbvI BcgI
BcnI BfaI BglII BlnI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII