##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Aor51HI AscI Asp700I
Asp718I AviII AvrII BbeI BciVI
BcnI BfrI BlnI BlpI Bpu1102I
BsaHI BsiEI BsiWI BsmI BspDI
BssHII BssSI BstAPI BstBI BstUI
CelII ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoRV EcoT22I
FauI FseI FspI Hin1I HpaI
KasI KpnI KspI MluI MspA1I
MvnI NarI NciI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 2763 TCCGGA T^CCGGA
AhdI 2593 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2804 GTGCAC G^TGCAC
ApaLI 2804 GTGCAC G^TGCAC
AspEI 2593 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1431 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2397 GGATCC G^GATCC
BbrPI 2968 CACGTG CAC^GTG
BcgI 1345 GCAGGAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3439 AGATCT A^GATCT
BsaWI 2763 TCCGGA [AT]^CCGG[AT]
BseAI 2763 TCCGGA T^CCGGA
BspEI 2763 TCCGGA T^CCGGA
BsrDI 3132 GCAATG GCAATG(2/0)
Bst1107I 2974 GTATAC GTA^TAC
BstZ17I 2974 GTATAC GTA^TAC
DsaI 2233 CCATGG C^C[GA][CT]GG
Eam1105I 2593 GACACATAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2216 GAATTC G^AATTC
HaeII 1411 AGCGCC [GA]GCGC^[CT]
HgaI 383 GACGC GACGC(5/10)
HincII 1056 GTCAAC GT[CT]^[GA]AC
HindII 1056 GTCAAC GT[CT]^[GA]AC
HindIII 42 AAGCTT A^AGCTT
MroI 2763 TCCGGA T^CCGGA
MslI 100 CATTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2239 GCCGGC GCC^GGC
NcoI 2233 CCATGG C^CATGG
NgoMIV 2239 GCCGGC G^CCGGC
NheI 1954 GCTAGC G^CTAGC
PflFI 1431 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2968 CACGTG CAC^GTG
PmlI 2968 CACGTG CAC^GTG
PstI 870 CTGCAG CTGCA^G
SphI 3224 GCATGC GCATG^C
SwaI 3933 ATTTAAAT ATTT^AAAT
Tth111I 1431 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 313 TCTAGA T^CTAGA
XcmI 399 CCAACGGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2143 GTCTAC GT^[AC][GT]AC
2974 GTATAC
AflIII 2724 ACATGT A^C[GA][CT]GT
3518 ACATGT
AocI 1222 CCTGAGG CC^T[ACGT]AGG
3309 CCTCAGG
ApaI 1038 GGGCCC GGGCC^C
3040 GGGCCC
AseI 2643 ATTAAT AT^TAAT
3706 ATTAAT
AsnI 2643 ATTAAT AT^TAAT
3706 ATTAAT
BalI 1249 TGGCCA TGG^CCA
3283 TGGCCA
BanI 389 GGCACC G^G[CT][GA]CC
2229 GGCACC
BglI 689 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
791 GCCACACTGGC
BsaAI 2801 TACGTG [CT]AC^GT[GA]
2968 CACGTG
BsaBI 1075 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1081 GATCGTGATC
BsaI 1006 GAGACC GGTCTC(1/5)
3293 GGTCTC
Bsp1407I 2692 TGTACA T^GTACA
2946 TGTACA
BspHI 2356 TCATGA T^CATGA
4133 TCATGA
BspLU11I 2724 ACATGT A^CATGT
3518 ACATGT
BsrBI 2630 GAGCGG CCGCTC(-3/-3)
3178 GAGCGG
BsrFI 960 GCCGGT [GA]^CCGG[CT]
2239 GCCGGC
BsrGI 2692 TGTACA T^GTACA
2946 TGTACA
BstEII 404 GGTGACC G^GT[ACGT]ACC
602 GGTGACC
BstPI 404 GGTGACC G^GT[ACGT]ACC
602 GGTGACC
Bsu36I 1222 CCTGAGG CC^T[ACGT]AGG
3309 CCTCAGG
Cfr10I 960 GCCGGT [GA]^CCGG[CT]
2239 GCCGGC
DraIII 198 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
594 CACAACGTG
Eco81I 1222 CCTGAGG CC^T[ACGT]AGG
3309 CCTCAGG
EcoNI 31 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3999 CCTCCTTGAGG
EcoO65I 404 GGTGACC G^GT[ACGT]ACC
602 GGTGACC
MamI 1075 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1081 GATCGTGATC
MfeI 806 CAATTG C^AATTG
1554 CAATTG
MluNI 1249 TGGCCA TGG^CCA
3283 TGGCCA
MscI 1249 TGGCCA TGG^CCA
3283 TGGCCA
MunI 806 CAATTG C^AATTG
1554 CAATTG
NdeI 71 CATATG CA^TATG
1768 CATATG
PshBI 2643 ATTAAT AT^TAAT
3706 ATTAAT
RcaI 2356 TCATGA T^CATGA
4133 TCATGA
SacI 134 GAGCTC GAGCT^C
2273 GAGCTC
ScaI 1802 AGTACT AGT^ACT
1996 AGTACT
SexAI 30 ACCTGGT A^CC[AT]GGT
3689 ACCTGGT
SspBI 2692 TGTACA T^GTACA
2946 TGTACA
SspI 2407 AATATT AAT^ATT
3532 AATATT
TaqI 1024 TCGA T^CGA
3176 TCGA
TthHB8I 1024 TCGA T^CGA
3176 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BclI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII