##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AccIII      AflIII      AgeI        AhdI        
Aor51HI     AscI        AspEI       AviII       AvrII       
BamHI       BbsI        BbvCI       BclI        BlnI        
BpuAI       BsaBI       BsaWI       BseAI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrGI       BssSI       BstBI       BstEII      BstPI       
BstXI       ClaI        CpoI        DraIII      DrdI        
Eam1105I    Eco47III    EcoNI       EcoO65I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
MamI        MluI        MroI        NdeI        NotI        
NruI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmeI        PshAI       PvuI        PvuII       
RsrII       SalI        SapI        SbfI        SexAI       
SfiI        SfuI        SnaBI       SpeI        Sse8387I    
SspBI       StuI        SwaI        Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         3696  GTATAC               GT^[AC][GT]AC
AclI         2087  AACGTT               AA^CGTT
Alw44I       2485  GTGCAC               G^TGCAC
AocI          594  CCTAAGG              CC^T[ACGT]AGG
ApaLI        2485  GTGCAC               G^TGCAC
AseI          499  ATTAAT               AT^TAAT
AsnI          499  ATTAAT               AT^TAAT
AspI         2317  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        2710  CACGTG               CAC^GTG
BcgI           60  GCAGGCGGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1617  GTATCC               GTATCC(6/5)
BlpI         3914  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3914  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2710  CACGTG               [CT]AC^GT[GA]
BsmBI        2498  GAGACG               CGTCTC(1/5)
BspMI        2540  ACCTGC               ACCTGC(4/8)
BsrFI         109  GCCGGC               [GA]^CCGG[CT]
Bst1107I     3696  GTATAC               GTA^TAC
BstZ17I      3696  GTATAC               GTA^TAC
Bsu36I        594  CCTAAGG              CC^T[ACGT]AGG
CelII        3914  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        109  GCCGGC               [GA]^CCGG[CT]
EagI           93  CGGCCG               C^GGCCG
EclXI          93  CGGCCG               C^GGCCG
Eco52I         93  CGGCCG               C^GGCCG
Eco81I        594  CCTAAGG              CC^T[ACGT]AGG
EcoRV         714  GATATC               GAT^ATC
HindIII      2520  AAGCTT               A^AGCTT
KspI          309  CCGCGG               CCGC^GG
NaeI          109  GCCGGC               GCC^GGC
NgoMIV        109  GCCGGC               G^CCGGC
NheI          289  GCTAGC               G^CTAGC
PflFI        2317  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2710  CACGTG               CAC^GTG
PmlI         2710  CACGTG               CAC^GTG
PshBI         499  ATTAAT               AT^TAAT
Psp1406I     2087  AACGTT               AA^CGTT
SacI         1463  GAGCTC               GAGCT^C
SacII         309  CCGCGG               CCGC^GG
ScaI         2003  AGTACT               AGT^ACT
SgrAI         108  CGCCGGCG             C[GA]^CCGG[CT]G
Tth111I      2317  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2402  CCAAAGGTGATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        821  GGTACC               G^GTACC
             1048  GGTACC              
AflII        1009  CTTAAG               C^TTAAG
             3788  CTTAAG              
AlwNI        1947  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3134  CAGATGCTG           
Asp700I      1228  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2123  GAATTGATTC          
Asp718I       821  GGTACC               G^GTACC
             1048  GGTACC              
AspHI        1463  GAGCTC               G[AT]GC[AT]^C
             2485  GTGCAC              
BfrI         1009  CTTAAG               C^TTAAG
             3788  CTTAAG              
BglI           95  GCCGGGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              109  GCCGGCGGGGC         
BglII        2874  AGATCT               A^GATCT
             3294  AGATCT              
BpmI         2675  CTGGAG               CTGGAG(16/14)
             3455  CTGGAG              
BsaI         2289  GGTCTC               GGTCTC(1/5)
             2331  GGTCTC              
BsgI           58  GTGCAG               GTGCAG(16/14)
             2462  GTGCAG              
BsiEI          93  CGGCCG               CG[GA][CT]^CG
              341  CGGTCG              
BsiHKAI      1463  GAGCTC               G[AT]GC[AT]^C
             2485  GTGCAC              
BspHI        1086  TCATGA               T^CATGA
             2276  TCATGA              
BsrDI         695  GCAATG               GCAATG(2/0)
              960  GCAATG              
BssHII          3  GCGCGC               G^CGCGC
              220  GCGCGC              
BstAPI        787  GCAGATGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1600  GCATTCTTTGC         
DraI          987  TTTAAA               TTT^AAA
             3568  TTTAAA              
EarI          423  GAAGAG               CTCTTC(1/4)
             2729  GAAGAG              
EcoT22I      1598  ATGCAT               ATGCA^T
             2795  ATGCAT              
KpnI          821  GGTACC               GGTAC^C
             1048  GGTACC              
Ksp632I       423  GAAGAG               CTCTTC(1/4)
             2729  GAAGAG              
MaeII        2088  ACGT                 A^CGT
             2711  ACGT                
MfeI         3509  CAATTG               C^AATTG
             3999  CAATTG              
MspA1I        231  CAGCGG               C[AC]G^C[GT]G
              309  CCGCGG              
MunI         3509  CAATTG               C^AATTG
             3999  CAATTG              
NsiI         1598  ATGCAT               ATGCA^T
             2795  ATGCAT              
RcaI         1086  TCATGA               T^CATGA
             2276  TCATGA              
SphI          458  GCATGC               GCATG^C
             3906  GCATGC              
XbaI          547  TCTAGA               T^CTAGA
             3889  TCTAGA              
XmnI         1228  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2123  GAATTGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApaI        ApoI        AvaI        
AvaII       BalI        BanI        BanII       BbeI        
BbvI        BcnI        BfaI        BmyI        BsaHI       
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        MaeI        MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MvnI        
MwoI        NarI        NciI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        SmaI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmaCI       XmaI