##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AflII AgeI ApaI AscI
AseI AsnI Asp700I AvrII BalI
BamHI BbvCI BciVI BfrI BlnI
BsaBI BsiWI BspDI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EcoT22I FseI HpaI MamI
MfeI MluI MluNI MscI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1059 GGTACC G^GTACC
AccI 3094 GTCGAC GT^[AC][GT]AC
AccIII 3480 TCCGGA T^CCGGA
AhdI 1936 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3798 AGCGCT AGC^GCT
Asp718I 1059 GGTACC G^GTACC
AspEI 1936 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1982 TGCGCA TGC^GCA
BclI 2376 TGATCA T^GATCA
BlpI 2393 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2393 GCTGAGC GC^T[ACGT]AGC
BsaI 212 GAGACC GGTCTC(1/5)
BsaWI 3480 TCCGGA [AT]^CCGG[AT]
BseAI 3480 TCCGGA T^CCGGA
BsmI 3141 GCATTC GAATGC(1/-1)
BspEI 3480 TCCGGA T^CCGGA
BsrBI 281 GAGCGG CCGCTC(-3/-3)
BssSI 1418 CTCGTG CACGAG(-5/-1)
BstEII 1810 GGTTACC G^GT[ACGT]ACC
BstPI 1810 GGTTACC G^GT[ACGT]ACC
CelII 2393 GCTGAGC GC^T[ACGT]AGC
DraIII 3580 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 129 CGGCCG C^GGCCG
Eam1105I 1936 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1629 GAAGAG CTCTTC(1/4)
EclXI 129 CGGCCG C^GGCCG
Eco47III 3798 AGCGCT AGC^GCT
Eco52I 129 CGGCCG C^GGCCG
EcoO65I 1810 GGTTACC G^GT[ACGT]ACC
EcoRI 3349 GAATTC G^AATTC
EcoRV 3359 GATATC GAT^ATC
FbaI 2376 TGATCA T^GATCA
FspI 1982 TGCGCA TGC^GCA
HincII 3094 GTCGAC GT[CT]^[GA]AC
HindII 3094 GTCGAC GT[CT]^[GA]AC
KpnI 1059 GGTACC GGTAC^C
Ksp632I 1629 GAAGAG CTCTTC(1/4)
KspI 132 CCGCGG CCGC^GG
MroI 3480 TCCGGA T^CCGGA
NcoI 855 CCATGG C^CATGG
NspI 561 ACATGC [GA]CATG^[CT]
PaeR7I 1579 CTCGAG C^TCGAG
PpuMI 3179 GGGACCC [GA]G^G[AT]CC[CT]
SacII 132 CCGCGG CCGC^GG
SalI 3094 GTCGAC G^TCGAC
SapI 1629 GAAGAGC GCTCTTC(1/4)
SexAI 1806 ACCTGGT A^CC[AT]GGT
SfiI 2827 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 216 CCCGGG CCC^GGG
XhoI 1579 CTCGAG C^TCGAG
XmaCI 216 CCCGGG C^CCGGG
XmaI 216 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1166 GACGTC GACGT^C
3530 GACGTC
AcsI 419 AAATTT [GA]^AATT[CT]
3349 GAATTC
ApoI 419 AAATTT [GA]^AATT[CT]
3349 GAATTC
BbeI 6 GGCGCC GGCGC^C
2447 GGCGCC
BcgI 1860 CGAGGGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2291 GCAGCAGTCTCG
BglII 1948 AGATCT A^GATCT
3192 AGATCT
BsiEI 129 CGGCCG CG[GA][CT]^CG
1230 CGGTCG
BsmBI 1410 CGTCTC CGTCTC(1/5)
2556 GAGACG
Bsp1407I 403 TGTACA T^GTACA
2004 TGTACA
BspHI 478 TCATGA T^CATGA
2133 TCATGA
BsrGI 403 TGTACA T^GTACA
2004 TGTACA
DraI 416 TTTAAA TTT^AAA
890 TTTAAA
EaeI 129 CGGCCG [CT]^GGCC[GA]
3492 CGGCCA
HindIII 543 AAGCTT A^AGCTT
1004 AAGCTT
KasI 6 GGCGCC G^GCGCC
2447 GGCGCC
NaeI 1769 GCCGGC GCC^GGC
2475 GCCGGC
NarI 6 GGCGCC GG^CGCC
2447 GGCGCC
NgoMIV 1769 GCCGGC G^CCGGC
2475 GCCGGC
RcaI 478 TCATGA T^CATGA
2133 TCATGA
SfoI 6 GGCGCC GGC^GCC
2447 GGCGCC
SmlI 1579 CTCGAG C^T[CT][GA]AG
2260 CTCAAG
SspBI 403 TGTACA T^GTACA
2004 TGTACA
SspI 886 AATATT AAT^ATT
2325 AATATT
StuI 1396 AGGCCT AGG^CCT
3000 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaLI AspHI AspI AvaI AvaII
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI PmaCI PmlI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XcmI XhoII