##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AclI AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AviII BamHI BbrPI BcgI BglI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
FseI FspI HindIII HpaI KspI
MamI MluI MroI NdeI NotI
NruI NspV PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SbfI ScaI SfiI SfuI
SnaBI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2358 GACGTC GACGT^C
AccI 27 GTCGAC GT^[AC][GT]AC
AspI 3182 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 213 CCTAGG C^CTAGG
BclI 3176 TGATCA T^GATCA
BlnI 213 CCTAGG C^CTAGG
BlpI 127 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 127 GCTGAGC GC^T[ACGT]AGC
BsmBI 2562 CGTCTC CGTCTC(1/5)
BssSI 1213 CACGAG CACGAG(-5/-1)
BstXI 3190 CCAGCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 127 GCTGAGC GC^T[ACGT]AGC
DraI 1688 TTTAAA TTT^AAA
DraIII 1976 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3508 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1300 ATGCAT ATGCA^T
FbaI 3176 TGATCA T^GATCA
MfeI 2747 CAATTG C^AATTG
MunI 2747 CAATTG C^AATTG
NaeI 204 GCCGGC GCC^GGC
NgoMIV 204 GCCGGC G^CCGGC
NheI 1814 GCTAGC G^CTAGC
NsiI 1300 ATGCAT ATGCA^T
PacI 3620 TTAATTAA TTAAT^TAA
PaeR7I 2323 CTCGAG C^TCGAG
PflFI 3182 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3126 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 361 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 27 GTCGAC G^TCGAC
SapI 165 GCTCTTC GCTCTTC(1/4)
SexAI 4113 ACCAGGT A^CC[AT]GGT
SgrAI 203 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 3079 CCCGGG CCC^GGG
SpeI 2070 ACTAGT A^CTAGT
SspI 747 AATATT AAT^ATT
Tth111I 3182 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2323 CTCGAG C^TCGAG
XmaCI 3079 CCCGGG C^CCGGG
XmaI 3079 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 3075 GGTACC G^GTACC
4110 GGTACC
AccII 41 CGCG CG^CG
330 CGCG
Alw44I 1798 GTGCAC G^TGCAC
1940 GTGCAC
AlwNI 232 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
3180 CAGACACTG
ApaLI 1798 GTGCAC G^TGCAC
1940 GTGCAC
AseI 3619 ATTAAT AT^TAAT
3623 ATTAAT
AsnI 3619 ATTAAT AT^TAAT
3623 ATTAAT
Asp700I 1595 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1695 GAACTGATTC
Asp718I 3075 GGTACC G^GTACC
4110 GGTACC
BalI 2239 TGGCCA TGG^CCA
2714 TGGCCA
BanII 2919 GAGCTC G[GA]GC[CT]^C
3647 GAGCTC
BbeI 201 GGCGCC GGCGC^C
207 GGCGCC
BbvCI 836 CCTCAGC CCTCAGC(-5/-2)
3159 GCTGAGG
BciVI 2015 GGATAC GTATCC(6/5)
3242 GGATAC
BglII 1015 AGATCT A^GATCT
1110 AGATCT
BsaI 319 GGTCTC GGTCTC(1/5)
2433 GGTCTC
BspMI 2679 ACCTGC ACCTGC(4/8)
316 GCAGGT
BsrBI 287 CCGCTC CCGCTC(-3/-3)
3350 GAGCGG
BsrFI 204 GCCGGC [GA]^CCGG[CT]
3805 ACCGGC
BstAPI 2339 GCACGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3020 GCAACTTATGC
BstUI 41 CGCG CG^CG
330 CGCG
Cfr10I 204 GCCGGC [GA]^CCGG[CT]
3805 ACCGGC
DraII 3126 AGGACCC [GA]G^G[ACGT]CC[CT]
3463 AGGGCCT
DsaI 2236 CCATGG C^C[GA][CT]GG
3849 CCATGG
EaeI 2239 TGGCCA [CT]^GGCC[GA]
2714 TGGCCA
EcoO109I 3126 AGGACCC [GA]G^G[ACGT]CC[CT]
3463 AGGGCCT
HincII 27 GTCGAC GT[CT]^[GA]AC
591 GTTGAC
HindII 27 GTCGAC GT[CT]^[GA]AC
591 GTTGAC
KasI 201 GGCGCC G^GCGCC
207 GGCGCC
KpnI 3075 GGTACC GGTAC^C
4110 GGTACC
MluNI 2239 TGGCCA TGG^CCA
2714 TGGCCA
MscI 2239 TGGCCA TGG^CCA
2714 TGGCCA
MvnI 41 CGCG CG^CG
330 CGCG
NarI 201 GGCGCC GG^CGCC
207 GGCGCC
NcoI 2236 CCATGG C^CATGG
3849 CCATGG
PflMI 1037 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1975 CCACTGTGTGG
PshBI 3619 ATTAAT AT^TAAT
3623 ATTAAT
PvuII 1350 CAGCTG CAG^CTG
3764 CAGCTG
SacI 2919 GAGCTC GAGCT^C
3647 GAGCTC
SfoI 201 GGCGCC GGC^GCC
207 GGCGCC
StuI 631 AGGCCT AGG^CCT
3398 AGGCCT
Van91I 1037 CCAATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1975 CCACTGTGTGG
XbaI 250 TCTAGA T^CTAGA
1628 TCTAGA
XcmI 2762 CCAGAGTAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3723 CCATGCGTTGTTTGG
XmnI 1595 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1695 GAACTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BbsI BbvI BcnI
BfaI BfrI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DrdI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII