##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BseAI BsiWI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoT22I FseI FspI HpaI
KasI KspI MluI MroI NaeI
NarI NgoMIV NheI NotI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 418 GACGTC GACGT^C
Acc65I 1748 GGTACC G^GTACC
AccI 3824 GTCTAC GT^[AC][GT]AC
AccII 556 CGCG CG^CG
Asp718I 1748 GGTACC G^GTACC
AspI 391 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3712 CTCGAG C^[CT]CG[GA]G
BanI 1748 GGTACC G^G[CT][GA]CC
BcnI 984 CCGGG CC^[GC]GG
BglI 3709 GCCCTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1142 AGATCT A^GATCT
BsaAI 2318 CACGTA [CT]AC^GT[GA]
BsaBI 1513 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2550 ACCGGA [AT]^CCGG[AT]
BsmI 2656 GCATTC GAATGC(1/-1)
BsoBI 3712 CTCGAG C^[CT]CG[GA]G
BspLU11I 3057 ACATGT A^CATGT
BspMI 3042 ACCTGC ACCTGC(4/8)
BstUI 556 CGCG CG^CG
BstYI 1142 AGATCT [GA]^GATC[CT]
DraIII 2287 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 301 CGGCCG C^GGCCG
EclXI 301 CGGCCG C^GGCCG
Eco52I 301 CGGCCG C^GGCCG
EcoRI 2688 GAATTC G^AATTC
EcoRV 574 GATATC GAT^ATC
FauI 1087 GCGGG CCCGC(4/6)
KpnI 1748 GGTACC GGTAC^C
MamI 1513 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1142 AGATCT [GA]^GATC[CT]
MvnI 556 CGCG CG^CG
NciI 984 CCGGG CC^[GC]GG
NdeI 2167 CATATG CA^TATG
NruI 555 TCGCGA TCG^CGA
PaeR7I 3712 CTCGAG C^TCGAG
PflFI 391 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1221 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2059 CTGCAG CTGCA^G
SacI 1741 GAGCTC GAGCT^C
ScaI 1268 AGTACT AGT^ACT
SspI 3910 AATATT AAT^ATT
Tth111I 391 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3629 CCACTGCTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3712 CTCGAG C^TCGAG
XhoII 1142 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 839 GTGCAC G^TGCAC
3150 GTGCAC
ApaLI 839 GTGCAC G^TGCAC
3150 GTGCAC
AseI 496 ATTAAT AT^TAAT
3943 ATTAAT
AsnI 496 ATTAAT AT^TAAT
3943 ATTAAT
BalI 1109 TGGCCA TGG^CCA
3683 TGGCCA
BanII 1741 GAGCTC G[GA]GC[CT]^C
3625 GAGCCC
BclI 3341 TGATCA T^GATCA
4141 TGATCA
BlpI 1116 GCTGAGC GC^T[ACGT]AGC
2121 GCTCAGC
Bpu1102I 1116 GCTGAGC GC^T[ACGT]AGC
2121 GCTCAGC
BsiEI 301 CGGCCG CG[GA][CT]^CG
2361 CGACCG
BspHI 1682 TCATGA T^CATGA
3768 TCATGA
BssSI 1395 CACGAG CACGAG(-5/-1)
847 CTCGTG
BstXI 2834 CCAGGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3005 CCACCCAAGTGG
CelII 1116 GCTGAGC GC^T[ACGT]AGC
2121 GCTCAGC
DraI 3107 TTTAAA TTT^AAA
3551 TTTAAA
EcoNI 2154 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2346 CCTGAACCAGG
FbaI 3341 TGATCA T^GATCA
4141 TGATCA
HaeII 898 AGCGCC [GA]GCGC^[CT]
2916 GGCGCT
HapII 984 CCGG C^CGG
2551 CCGG
HincII 2671 GTCAAC GT[CT]^[GA]AC
2860 GTCAAC
HindII 2671 GTCAAC GT[CT]^[GA]AC
2860 GTCAAC
HpaII 984 CCGG C^CGG
2551 CCGG
MluNI 1109 TGGCCA TGG^CCA
3683 TGGCCA
MscI 1109 TGGCCA TGG^CCA
3683 TGGCCA
MslI 921 CATTGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3006 CACCCAAGTG
MspI 984 CCGG C^CGG
2551 CCGG
PshBI 496 ATTAAT AT^TAAT
3943 ATTAAT
RcaI 1682 TCATGA T^CATGA
3768 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HgaI HhaI
Hin1I HinP1I HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MspA1I MunI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmnI