##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AgeI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI AviII AvrII BamHI BbeI
BbrPI BlnI BsiWI BspDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI EagI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV FseI FspI
KasI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfoI SfuI
SgrAI SmaI SpeI SwaI XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 997 GGTACC G^GTACC
AccIII 4009 TCCGGA T^CCGGA
AcsI 378 AAATTC [GA]^AATT[CT]
AflII 1734 CTTAAG C^TTAAG
AhdI 3531 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3952 AGCGCT AGC^GCT
ApoI 378 AAATTC [GA]^AATT[CT]
Asp700I 1358 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 997 GGTACC G^GTACC
AspEI 3531 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 1761 GCTGAGG CCTCAGC(-5/-2)
BciVI 1389 GGATAC GTATCC(6/5)
BclI 2191 TGATCA T^GATCA
BfrI 1734 CTTAAG C^TTAAG
BglI 345 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3749 AGATCT A^GATCT
BlpI 3868 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3868 GCTCAGC GC^T[ACGT]AGC
BsaBI 659 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4009 TCCGGA T^CCGGA
BsiEI 3897 CGGTCG CG[GA][CT]^CG
BsmBI 3555 GAGACG CGTCTC(1/5)
BspEI 4009 TCCGGA T^CCGGA
BspLU11I 1108 ACATGT A^CATGT
BssSI 2394 CTCGTG CACGAG(-5/-1)
CelII 3868 GCTCAGC GC^T[ACGT]AGC
DraI 3825 TTTAAA TTT^AAA
Eam1105I 3531 GACGAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3952 AGCGCT AGC^GCT
FauI 1269 GCGGG CCCGC(4/6)
FbaI 2191 TGATCA T^GATCA
HindIII 4090 AAGCTT A^AGCTT
KpnI 997 GGTACC GGTAC^C
MamI 659 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4009 TCCGGA T^CCGGA
NaeI 2989 GCCGGC GCC^GGC
NcoI 1468 CCATGG C^CATGG
NgoMIV 2989 GCCGGC G^CCGGC
PshAI 612 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1245 CAGCTG CAG^CTG
SacI 3620 GAGCTC GAGCT^C
SbfI 2945 CCTGCAGG CCTGCA^GG
ScaI 3098 AGTACT AGT^ACT
SfiI 344 GGCCTTTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 2642 TACGTA TAC^GTA
SphI 2563 GCATGC GCATG^C
Sse8387I 2945 CCTGCAGG CCTGCA^GG
SspI 3129 AATATT AAT^ATT
StuI 3990 AGGCCT AGG^CCT
XmnI 1358 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 885 GTAGAC GT^[AC][GT]AC
3218 GTCTAC
AcyI 2449 GACGCC G[GA]^CG[CT]C
3049 GACGCC
AflIII 1108 ACATGT A^C[GA][CT]GT
3670 ACGTGT
AspHI 2779 GTGCTC G[AT]GC[AT]^C
3620 GAGCTC
AspI 282 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1983 GACCCTGTC
BcgI 3196 CGACCTCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1700 GCACACCGTTCG
BfaI 2427 CTAG C^TAG
3994 CTAG
BsaHI 2449 GACGCC G[GA]^CG[CT]C
3049 GACGCC
BsaWI 1386 ACCGGA [AT]^CCGG[AT]
4009 TCCGGA
BsiHKAI 2779 GTGCTC G[AT]GC[AT]^C
3620 GAGCTC
BspHI 1822 TCATGA T^CATGA
3601 TCATGA
BsrBI 2486 GAGCGG CCGCTC(-3/-3)
3629 GAGCGG
BsrFI 1380 ACCGGC [GA]^CCGG[CT]
2989 GCCGGC
BstYI 1087 AGATCC [GA]^GATC[CT]
3749 AGATCT
Cfr10I 1380 ACCGGC [GA]^CCGG[CT]
2989 GCCGGC
EcoT22I 1476 ATGCAT ATGCA^T
3851 ATGCAT
HaeII 2637 GGCGCT [GA]GCGC^[CT]
3952 AGCGCT
Hin1I 2449 GACGCC G[GA]^CG[CT]C
3049 GACGCC
MaeI 2427 CTAG C^TAG
3994 CTAG
MflI 1087 AGATCC [GA]^GATC[CT]
3749 AGATCT
NsiI 1476 ATGCAT ATGCA^T
3851 ATGCAT
PflFI 282 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1983 GACCCTGTC
PflMI 366 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2784 CCAGGACTTGG
RcaI 1822 TCATGA T^CATGA
3601 TCATGA
SapI 3626 GAAGAGC GCTCTTC(1/4)
2475 GCTCTTC
Tth111I 282 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1983 GACCCTGTC
Van91I 366 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2784 CCAGGACTTGG
XhoII 1087 AGATCC [GA]^GATC[CT]
3749 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PaeR7I PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI