##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BcnI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BspMI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
BstAPI      BstBI       BstEII      BstPI       BstUI       
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     FauI        FseI        
FspI        HaeII       HapII       HhaI        HinP1I      
HpaII       KasI        KspI        MamI        MluI        
MroI        MspA1I      MspI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SwaI        TaqI        
Tth111I     TthHB8I     XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Acc65I       1981  GGTACC               G^GTACC
AcyI         2102  GGCGTC               G[GA]^CG[CT]C
AflIII       3594  ACATGT               A^C[GA][CT]GT
AlwNI        2939  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I      1981  GGTACC               G^GTACC
BanII        1575  GAGCTC               G[GA]GC[CT]^C
BciVI         829  GTATCC               GTATCC(6/5)
BsaHI        2102  GGCGTC               G[GA]^CG[CT]C
BsmBI        2104  CGTCTC               CGTCTC(1/5)
BspLU11I     3594  ACATGT               A^CATGT
BstXI        2333  CCAACAGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII        1212  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO109I     1212  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I         8  ATGCAT               ATGCA^T
HgaI         2103  GCGTC                GACGC(5/10)
Hin1I        2102  GGCGTC               G[GA]^CG[CT]C
HindIII      2009  AAGCTT               A^AGCTT
HpaI         2131  GTTAAC               GTT^AAC
HphI         1435  TCACC                GGTGA(8/7)
KpnI         1981  GGTACC               GGTAC^C
MfeI         2979  CAATTG               C^AATTG
MunI         2979  CAATTG               C^AATTG
NsiI            8  ATGCAT               ATGCA^T
PpuMI        1212  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          565  CTGCAG               CTGCA^G
SacI         1575  GAGCTC               GAGCT^C
ScaI          794  AGTACT               AGT^ACT
StuI         2446  AGGCCT               AGG^CCT
XbaI         3160  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AocI          833  CCTCAGG              CC^T[ACGT]AGG
             1645  CCTGAGG             
AspHI         494  GTGCTC               G[AT]GC[AT]^C
             1575  GAGCTC              
BalI          576  TGGCCA               TGG^CCA
             1151  TGGCCA              
BanI          185  GGTGCC               G^G[CT][GA]CC
             1981  GGTACC              
BclI          881  TGATCA               T^GATCA
             3771  TGATCA              
BglII        2194  AGATCT               A^GATCT
             2929  AGATCT              
BmyI          494  GTGCTC               G[AGT]GC[ACT]^C
             1575  GAGCTC              
BseRI         616  CTCCTC               GAGGAG(10/8)
             1781  CTCCTC              
BsgI         1009  GTGCAG               GTGCAG(16/14)
             3601  GTGCAG              
BsiHKAI       494  GTGCTC               G[AT]GC[AT]^C
             1575  GAGCTC              
BsmI         1077  GAATGC               GAATGC(1/-1)
             1145  GCATTC              
Bsp1286I      494  GTGCTC               G[AGT]GC[ACT]^C
             1575  GAGCTC              
Bsp1407I     1663  TGTACA               T^GTACA
             1814  TGTACA              
BsrGI        1663  TGTACA               T^GTACA
             1814  TGTACA              
Bst1107I      238  GTATAC               GTA^TAC
             1058  GTATAC              
BstZ17I       238  GTATAC               GTA^TAC
             1058  GTATAC              
Bsu36I        833  CCTCAGG              CC^T[ACGT]AGG
             1645  CCTGAGG             
Cac8I         574  GCTGGC               GC[ACGT]^[ACGT]GC
             1632  GCCAGC              
DrdI          632  GACGAGCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1797  GACTTCTAAGTC        
DsaI         2146  CCATGG               C^C[GA][CT]GG
             2232  CCATGG              
EaeI          576  TGGCCA               [CT]^GGCC[GA]
             1151  TGGCCA              
EarI         1967  CTCTTC               CTCTTC(1/4)
             2151  GAAGAG              
Eco81I        833  CCTCAGG              CC^T[ACGT]AGG
             1645  CCTGAGG             
EcoT14I      2146  CCATGG               C^C[AT][AT]GG
             2232  CCATGG              
FbaI          881  TGATCA               T^GATCA
             3771  TGATCA              
HincII       2131  GTTAAC               GT[CT]^[GA]AC
             2898  GTCAAC              
HindII       2131  GTTAAC               GT[CT]^[GA]AC
             2898  GTCAAC              
Ksp632I      1967  CTCTTC               CTCTTC(1/4)
             2151  GAAGAG              
MluNI         576  TGGCCA               TGG^CCA
             1151  TGGCCA              
MscI          576  TGGCCA               TGG^CCA
             1151  TGGCCA              
MwoI          657  GCTGCCATAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              862  GCCCTTCTTGC         
NcoI         2146  CCATGG               C^CATGG
             2232  CCATGG              
NdeI          285  CATATG               CA^TATG
             3570  CATATG              
PflMI         425  CCATCTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              579  CCACATTTTGG         
SpeI          514  ACTAGT               A^CTAGT
              797  ACTAGT              
SspBI        1663  TGTACA               T^GTACA
             1814  TGTACA              
StyI         2146  CCATGG               C^C[AT][AT]GG
             2232  CCATGG              
Van91I        425  CCATCTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              579  CCACATTTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        Asp700I     AvaII       
BbsI        BbvI        BfaI        BpmI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
BspHI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cfr13I      DdeI        DpnII       DraI        
EcoRI       EcoRII      EcoRV       Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MvaI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshBI       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SspI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       XmnI