##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I EcoO65I EcoT22I FbaI
FseI FspI Hin1I KasI KpnI
MluI MroI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI SspBI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 2423 ACGTGT A^C[GA][CT]GT
AhdI 2518 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 126 CCTCAGG CC^T[ACGT]AGG
Aor51HI 749 AGCGCT AGC^GCT
ApaI 212 GGGCCC GGGCC^C
AspEI 2518 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 364 TGGCCA TGG^CCA
BglII 1577 AGATCT A^GATCT
BsaBI 1630 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 545 GAATGC GAATGC(1/-1)
Bsu36I 126 CCTCAGG CC^T[ACGT]AGG
Eam1105I 2518 GACGGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 749 AGCGCT AGC^GCT
Eco81I 126 CCTCAGG CC^T[ACGT]AGG
EcoNI 249 CCTGGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1315 GAATTC G^AATTC
EcoRV 1210 GATATC GAT^ATC
HpaI 1831 GTTAAC GTT^AAC
KspI 1706 CCGCGG CCGC^GG
MamI 1630 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2154 CAATTG C^AATTG
MluNI 364 TGGCCA TGG^CCA
MscI 364 TGGCCA TGG^CCA
MunI 2154 CAATTG C^AATTG
NaeI 1248 GCCGGC GCC^GGC
NcoI 284 CCATGG C^CATGG
NgoMIV 1248 GCCGGC G^CCGGC
NspI 1919 ACATGC [GA]CATG^[CT]
PflMI 279 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 43 AGGACCC [GA]G^G[AT]CC[CT]
SacI 494 GAGCTC GAGCT^C
SacII 1706 CCGCGG CCGC^GG
SbfI 2074 CCTGCAGG CCTGCA^GG
SpeI 555 ACTAGT A^CTAGT
Sse8387I 2074 CCTGCAGG CCTGCA^GG
SspI 1285 AATATT AAT^ATT
Van91I 279 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 1367 CGCG CG^CG
1707 CGCG
AlwI 529 GGATC GGATC(4/5)
1424 GGATC
AvaI 1499 CCCGAG C^[CT]CG[GA]G
2170 CTCGGG
BbsI 259 GAAGAC GAAGAC(2/6)
1435 GTCTTC
BfaI 460 CTAG C^TAG
556 CTAG
BpuAI 259 GAAGAC GAAGAC(2/6)
1435 GTCTTC
BsaI 1756 GAGACC GGTCTC(1/5)
2011 GAGACC
BsoBI 1499 CCCGAG C^[CT]CG[GA]G
2170 CTCGGG
BsrDI 1172 GCAATG GCAATG(2/0)
2120 GCAATG
BsrFI 1248 GCCGGC [GA]^CCGG[CT]
2138 GCCGGT
BstUI 1367 CGCG CG^CG
1707 CGCG
BstYI 529 GGATCT [GA]^GATC[CT]
1577 AGATCT
Cfr10I 1248 GCCGGC [GA]^CCGG[CT]
2138 GCCGGT
DraII 43 AGGACCC [GA]G^G[ACGT]CC[CT]
1790 GGGGCCT
EaeI 364 TGGCCA [CT]^GGCC[GA]
1246 TGGCCG
EcoO109I 43 AGGACCC [GA]G^G[ACGT]CC[CT]
1790 GGGGCCT
HgaI 1356 GACGC GACGC(5/10)
1951 GACGC
HincII 1051 GTCAAC GT[CT]^[GA]AC
1831 GTTAAC
HindII 1051 GTCAAC GT[CT]^[GA]AC
1831 GTTAAC
HindIII 418 AAGCTT A^AGCTT
1483 AAGCTT
MaeI 460 CTAG C^TAG
556 CTAG
MflI 529 GGATCT [GA]^GATC[CT]
1577 AGATCT
MvnI 1367 CGCG CG^CG
1707 CGCG
SmlI 376 CTCAAG C^T[CT][GA]AG
2017 CTCAAG
StuI 388 AGGCCT AGG^CCT
2348 AGGCCT
XhoII 529 GGATCT [GA]^GATC[CT]
1577 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BssSI
BstAPI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I