##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccII AflIII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspHI AspI
AviII AvrII BbrPI BciVI BlnI
BsaAI BsaBI BsiEI BsiHKAI BsiWI
BspDI BspLU11I BssHII Bst1107I BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FseI
FspI HgaI HindIII KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PstI PvuI RsrII SacI
SacII SbfI SexAI SfiI SgrAI
SmaI SnaBI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 802 GTCGAC GT^[AC][GT]AC
AccIII 2067 TCCGGA T^CCGGA
AcyI 27 GGCGCC G[GA]^CG[CT]C
AflII 1786 CTTAAG C^TTAAG
AhdI 2933 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2537 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 2933 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 4416 CCCGAG C^[CT]CG[GA]G
BalI 1628 TGGCCA TGG^CCA
BanI 27 GGCGCC G^G[CT][GA]CC
BanII 3835 GAGCCC G[GA]GC[CT]^C
BbeI 27 GGCGCC GGCGC^C
BbvCI 4258 CCTCAGC CCTCAGC(-5/-2)
BcnI 1076 CCGGG CC^[GC]GG
BfrI 1786 CTTAAG C^TTAAG
BglI 3157 GCCTAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4420 AGATCT A^GATCT
BmyI 3835 GAGCCC G[AGT]GC[ACT]^C
BsaHI 27 GGCGCC G[GA]^CG[CT]C
BsaWI 2067 TCCGGA [AT]^CCGG[AT]
BseAI 2067 TCCGGA T^CCGGA
BsmBI 142 GAGACG CGTCTC(1/5)
BsoBI 4416 CCCGAG C^[CT]CG[GA]G
Bsp1286I 3835 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 913 TGTACA T^GTACA
BspEI 2067 TCCGGA T^CCGGA
BsrBI 104 CCGCTC CCGCTC(-3/-3)
BsrFI 2664 GCCGGT [GA]^CCGG[CT]
BsrGI 913 TGTACA T^GTACA
BssSI 377 CACGAG CACGAG(-5/-1)
BstAPI 2536 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3174 GGTCACC G^GT[ACGT]ACC
BstPI 3174 GGTCACC G^GT[ACGT]ACC
Cfr10I 2664 GCCGGT [GA]^CCGG[CT]
DsaI 282 CCGTGG C^C[GA][CT]GG
Eam1105I 2933 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1714 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3174 GGTCACC G^GT[ACGT]ACC
EcoT22I 1977 ATGCAT ATGCA^T
Hin1I 27 GGCGCC G[GA]^CG[CT]C
HpaI 2488 GTTAAC GTT^AAC
KasI 27 GGCGCC G^GCGCC
MluNI 1628 TGGCCA TGG^CCA
MroI 2067 TCCGGA T^CCGGA
MscI 1628 TGGCCA TGG^CCA
NarI 27 GGCGCC GG^CGCC
NciI 1076 CCGGG CC^[GC]GG
NsiI 1977 ATGCAT ATGCA^T
SalI 802 GTCGAC G^TCGAC
SapI 2420 GCTCTTC GCTCTTC(1/4)
SfoI 27 GGCGCC GGC^GCC
SspBI 913 TGTACA T^GTACA
XcmI 2307 CCACTTGAACGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AclI 2314 AACGTT AA^CGTT
2711 AACGTT
BclI 1237 TGATCA T^GATCA
1727 TGATCA
BlpI 1479 GCTAAGC GC^T[ACGT]AGC
4201 GCTAAGC
Bpu1102I 1479 GCTAAGC GC^T[ACGT]AGC
4201 GCTAAGC
BspHI 2794 TCATGA T^CATGA
4249 TCATGA
BsrDI 1666 GCAATG GCAATG(2/0)
3929 GCAATG
CelII 1479 GCTAAGC GC^T[ACGT]AGC
4201 GCTAAGC
DraII 165 GGGACCC [GA]G^G[ACGT]CC[CT]
619 AGGACCT
EcoO109I 165 GGGACCC [GA]G^G[ACGT]CC[CT]
619 AGGACCT
FbaI 1237 TGATCA T^GATCA
1727 TGATCA
HaeII 20 GGCGCT [GA]GCGC^[CT]
27 GGCGCC
HincII 802 GTCGAC GT[CT]^[GA]AC
2488 GTTAAC
HindII 802 GTCGAC GT[CT]^[GA]AC
2488 GTTAAC
MaeII 2315 ACGT A^CGT
2712 ACGT
MslI 3998 CACTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4055 CATAGTGATG
PpuMI 165 GGGACCC [GA]G^G[AT]CC[CT]
619 AGGACCT
Psp1406I 2314 AACGTT AA^CGTT
2711 AACGTT
PvuII 1143 CAGCTG CAG^CTG
3720 CAGCTG
RcaI 2794 TCATGA T^CATGA
4249 TCATGA
ScaI 558 AGTACT AGT^ACT
2581 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BamHI
BbsI BbvI BcgI BfaI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BspMI BsrI BssKI BstBI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI NspV
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
SpeI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII