##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AccIII      AclI        AcsI        ApoI        AscI        
AseI        AsnI        Asp700I     BbrPI       BsaBI       
BseAI       BsiWI       BsmI        BspDI       BspEI       
BspLU11I    BssHII      BstBI       BstXI       ClaI        
CpoI        DraI        EcoRI       EcoRV       EcoT22I     
FseI        HindIII     HpaI        MamI        MfeI        
MluI        MroI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SbfI        ScaI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspI        SwaI        TfiI        Tsp509I     XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        464  GGTACC               G^GTACC
AflII        3142  CTTAAG               C^TTAAG
AgeI         3036  ACCGGT               A^CCGGT
AhdI         3329  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        714  GTGCAC               G^TGCAC
ApaLI         714  GTGCAC               G^TGCAC
Asp718I       464  GGTACC               G^GTACC
AspEI        3329  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1814  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3373  CCTAGG               C^CTAGG
BcgI           66  GCACCGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1167  TGATCA               T^GATCA
BfrI         3142  CTTAAG               C^TTAAG
BlnI         3373  CCTAGG               C^CTAGG
BsaAI         589  CACGTA               [CT]AC^GT[GA]
BsaWI        3036  ACCGGT               [AT]^CCGG[AT]
BsmBI        2824  CGTCTC               CGTCTC(1/5)
BssSI        2444  CTCGTG               CACGAG(-5/-1)
Bst1107I     3242  GTATAC               GTA^TAC
BstZ17I      3242  GTATAC               GTA^TAC
EagI         2391  CGGCCG               C^GGCCG
Eam1105I     3329  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2391  CGGCCG               C^GGCCG
Eco52I       2391  CGGCCG               C^GGCCG
FbaI         1167  TGATCA               T^GATCA
KpnI          464  GGTACC               GGTAC^C
NaeI         3453  GCCGGC               GCC^GGC
NcoI          468  CCATGG               C^CATGG
NgoMIV       3453  GCCGGC               G^CCGGC
PaeR7I        329  CTCGAG               C^TCGAG
PflFI        1814  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        3036  ACCGGT               A^CCGGT
PshAI        1754  GACAAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SphI          669  GCATGC               GCATG^C
StuI         2466  AGGCCT               AGG^CCT
Tth111I      1814  GACGCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4480  TCTAGA               T^CTAGA
XhoI          329  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AflIII       2325  ACGTGT               A^C[GA][CT]GT
             3691  ACGTGT              
AocI         2917  CCTGAGG              CC^T[ACGT]AGG
             4135  CCTGAGG             
Aor51HI       729  AGCGCT               AGC^GCT
             2028  AGCGCT              
BamHI         376  GGATCC               G^GATCC
             1743  GGATCC              
BbeI         3671  GGCGCC               GGCGC^C
             3716  GGCGCC              
BbsI         3220  GAAGAC               GAAGAC(2/6)
             1889  GTCTTC              
BciVI        1183  GGATAC               GTATCC(6/5)
              908  GTATCC              
BfaI         3374  CTAG                 C^TAG
             4481  CTAG                
BglII        1665  AGATCT               A^GATCT
             3473  AGATCT              
BpuAI        3220  GAAGAC               GAAGAC(2/6)
             1889  GTCTTC              
BsiEI        1093  CGACCG               CG[GA][CT]^CG
             2391  CGGCCG              
Bsp1407I      441  TGTACA               T^GTACA
             2632  TGTACA              
BspHI         895  TCATGA               T^CATGA
             2691  TCATGA              
BsrDI        1699  CATTGC               GCAATG(2/0)
             3960  GCAATG              
BsrGI         441  TGTACA               T^GTACA
             2632  TGTACA              
Bsu36I       2917  CCTGAGG              CC^T[ACGT]AGG
             4135  CCTGAGG             
Eco47III      729  AGCGCT               AGC^GCT
             2028  AGCGCT              
Eco81I       2917  CCTGAGG              CC^T[ACGT]AGG
             4135  CCTGAGG             
EcoNI        2196  CCTCCTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4291  CCTTCCCCAGG         
HincII        118  GTCGAC               GT[CT]^[GA]AC
             1091  GTCGAC              
HindII        118  GTCGAC               GT[CT]^[GA]AC
             1091  GTCGAC              
KasI         3671  GGCGCC               G^GCGCC
             3716  GGCGCC              
KspI         3111  CCGCGG               CCGC^GG
             3712  CCGCGG              
MaeI         3374  CTAG                 C^TAG
             4481  CTAG                
MseI          135  TTAA                 T^TAA
             3143  TTAA                
NarI         3671  GGCGCC               GG^CGCC
             3716  GGCGCC              
RcaI          895  TCATGA               T^CATGA
             2691  TCATGA              
SacII        3111  CCGCGG               CCGC^GG
             3712  CCGCGG              
SalI          118  GTCGAC               G^TCGAC
             1091  GTCGAC              
SapI          802  GAAGAGC              GCTCTTC(1/4)
             1645  GCTCTTC             
SfoI         3671  GGCGCC               GGC^GCC
             3716  GGCGCC              
SmaI           18  CCCGGG               CCC^GGG
              251  CCCGGG              
SspBI         441  TGTACA               T^GTACA
             2632  TGTACA              
Tru9I         135  TTAA                 T^TAA
             3143  TTAA                
XmaCI          18  CCCGGG               C^CCGGG
              251  CCCGGG              
XmaI           18  CCCGGG               C^CCGGG
              251  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  124 enzymes) #
#########################################

AatII       AccI        AccII       AciI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApaI        
AspHI       AvaI        AvaII       AviII       BalI        
BanI        BanII       BbvCI       BbvI        BcnI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EarI        EcoO109I    EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      XcmI        XhoII