##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AspEI       AviII       BbeI        BbrPI       BcgI        
BciVI       BfrI        BglII       BlpI        Bpu1102I    
BsaBI       BsaI        BseAI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
Bsu36I      CelII       ClaI        CpoI        Eam1105I    
Eco47III    Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
KasI        MamI        MluI        MroI        NarI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SapI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        3056  GACGTC               GACGT^C
AflIII       2661  ACACGT               A^C[GA][CT]GT
AseI         2035  ATTAAT               AT^TAAT
AsnI         2035  ATTAAT               AT^TAAT
AvrII         184  CCTAGG               C^CTAGG
BamHI        2187  GGATCC               G^GATCC
BlnI          184  CCTAGG               C^CTAGG
BsaAI         886  TACGTG               [CT]AC^GT[GA]
BsiWI        1123  CGTACG               C^GTACG
BsmBI        3972  GAGACG               CGTCTC(1/5)
BspMI         843  ACCTGC               ACCTGC(4/8)
BssSI        1565  CTCGTG               CACGAG(-5/-1)
Bst1107I     3432  GTATAC               GTA^TAC
BstZ17I      3432  GTATAC               GTA^TAC
DraI         3589  TTTAAA               TTT^AAA
DrdI         1909  GACAGGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        3636  CCTGCCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII       1918  GTCAAC               GT[CT]^[GA]AC
HindII       1918  GTCAAC               GT[CT]^[GA]AC
KspI            2  CCGCGG               CCGC^GG
MfeI          522  CAATTG               C^AATTG
MunI          522  CAATTG               C^AATTG
NaeI         1961  GCCGGC               GCC^GGC
NgoMIV       1961  GCCGGC               G^CCGGC
NheI          360  GCTAGC               G^CTAGC
NspI         1955  ACATGC               [GA]CATG^[CT]
PflMI        3865  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2035  ATTAAT               AT^TAAT
SacII           2  CCGCGG               CCGC^GG
SfiI         3721  GGCCCTGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI         2993  AATATT               AAT^ATT
Van91I       3865  CCATCTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          791  CCAGAGAGAAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I       3456  GGTACC               G^GTACC
             3772  GGTACC              
AccI         2665  GTCTAC               GT^[AC][GT]AC
             3432  GTATAC              
Asp700I      1510  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1860  GAACACCTTC          
Asp718I      3456  GGTACC               G^GTACC
             3772  GGTACC              
AspHI        2267  GTGCTC               G[AT]GC[AT]^C
             2518  GAGCAC              
AspI         1162  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1542  GACAGAGTC           
BbsI         3064  GAAGAC               GAAGAC(2/6)
             3171  GTCTTC              
BbvCI         580  CCTCAGC              CCTCAGC(-5/-2)
             1756  CCTCAGC             
BpuAI        3064  GAAGAC               GAAGAC(2/6)
             3171  GTCTTC              
BsaWI         825  ACCGGA               [AT]^CCGG[AT]
              933  ACCGGA              
BsgI         1339  GTGCAG               GTGCAG(16/14)
             1568  GTGCAG              
BsiEI        1038  CGGCCG               CG[GA][CT]^CG
             1050  CGGCCG              
BsiHKAI      2267  GTGCTC               G[AT]GC[AT]^C
             2518  GAGCAC              
BsrBI        2292  CCGCTC               CCGCTC(-3/-3)
             2698  GAGCGG              
DraIII       1333  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3115  CACTCAGTG           
EagI         1038  CGGCCG               C^GGCCG
             1050  CGGCCG              
EclXI        1038  CGGCCG               C^GGCCG
             1050  CGGCCG              
Eco52I       1038  CGGCCG               C^GGCCG
             1050  CGGCCG              
KpnI         3456  GGTACC               GGTAC^C
             3772  GGTACC              
NcoI         3086  CCATGG               C^CATGG
             3346  CCATGG              
PflFI        1162  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1542  GACAGAGTC           
PpuMI        2063  AGGACCT              [GA]G^G[AT]CC[CT]
             2341  AGGACCC             
SmaI           81  CCCGGG               CCC^GGG
               91  CCCGGG              
Tth111I      1162  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1542  GACAGAGTC           
XmaCI          81  CCCGGG               C^CCGGG
               91  CCCGGG              
XmaI           81  CCCGGG               C^CCGGG
               91  CCCGGG              
XmnI         1510  GAATTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1860  GAACACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BclI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII