##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AspEI AviII BbeI BbrPI BcgI
BciVI BfrI BglII BlpI Bpu1102I
BsaBI BsaI BseAI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BstAPI BstBI BstEII BstPI
Bsu36I CelII ClaI CpoI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI MamI MluI MroI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 3056 GACGTC GACGT^C
AflIII 2661 ACACGT A^C[GA][CT]GT
AseI 2035 ATTAAT AT^TAAT
AsnI 2035 ATTAAT AT^TAAT
AvrII 184 CCTAGG C^CTAGG
BamHI 2187 GGATCC G^GATCC
BlnI 184 CCTAGG C^CTAGG
BsaAI 886 TACGTG [CT]AC^GT[GA]
BsiWI 1123 CGTACG C^GTACG
BsmBI 3972 GAGACG CGTCTC(1/5)
BspMI 843 ACCTGC ACCTGC(4/8)
BssSI 1565 CTCGTG CACGAG(-5/-1)
Bst1107I 3432 GTATAC GTA^TAC
BstZ17I 3432 GTATAC GTA^TAC
DraI 3589 TTTAAA TTT^AAA
DrdI 1909 GACAGGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3636 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1918 GTCAAC GT[CT]^[GA]AC
HindII 1918 GTCAAC GT[CT]^[GA]AC
KspI 2 CCGCGG CCGC^GG
MfeI 522 CAATTG C^AATTG
MunI 522 CAATTG C^AATTG
NaeI 1961 GCCGGC GCC^GGC
NgoMIV 1961 GCCGGC G^CCGGC
NheI 360 GCTAGC G^CTAGC
NspI 1955 ACATGC [GA]CATG^[CT]
PflMI 3865 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2035 ATTAAT AT^TAAT
SacII 2 CCGCGG CCGC^GG
SfiI 3721 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 2993 AATATT AAT^ATT
Van91I 3865 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 791 CCAGAGAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 3456 GGTACC G^GTACC
3772 GGTACC
AccI 2665 GTCTAC GT^[AC][GT]AC
3432 GTATAC
Asp700I 1510 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1860 GAACACCTTC
Asp718I 3456 GGTACC G^GTACC
3772 GGTACC
AspHI 2267 GTGCTC G[AT]GC[AT]^C
2518 GAGCAC
AspI 1162 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1542 GACAGAGTC
BbsI 3064 GAAGAC GAAGAC(2/6)
3171 GTCTTC
BbvCI 580 CCTCAGC CCTCAGC(-5/-2)
1756 CCTCAGC
BpuAI 3064 GAAGAC GAAGAC(2/6)
3171 GTCTTC
BsaWI 825 ACCGGA [AT]^CCGG[AT]
933 ACCGGA
BsgI 1339 GTGCAG GTGCAG(16/14)
1568 GTGCAG
BsiEI 1038 CGGCCG CG[GA][CT]^CG
1050 CGGCCG
BsiHKAI 2267 GTGCTC G[AT]GC[AT]^C
2518 GAGCAC
BsrBI 2292 CCGCTC CCGCTC(-3/-3)
2698 GAGCGG
DraIII 1333 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3115 CACTCAGTG
EagI 1038 CGGCCG C^GGCCG
1050 CGGCCG
EclXI 1038 CGGCCG C^GGCCG
1050 CGGCCG
Eco52I 1038 CGGCCG C^GGCCG
1050 CGGCCG
KpnI 3456 GGTACC GGTAC^C
3772 GGTACC
NcoI 3086 CCATGG C^CATGG
3346 CCATGG
PflFI 1162 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1542 GACAGAGTC
PpuMI 2063 AGGACCT [GA]G^G[AT]CC[CT]
2341 AGGACCC
SmaI 81 CCCGGG CCC^GGG
91 CCCGGG
Tth111I 1162 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1542 GACAGAGTC
XmaCI 81 CCCGGG C^CCGGG
91 CCCGGG
XmaI 81 CCCGGG C^CCGGG
91 CCCGGG
XmnI 1510 GAATTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1860 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AvaI AvaII BalI BanI BanII
BbvI BclI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII