##################################
# NO CUT ENZYMES ( 26 enzymes) #
##################################
AatII Acc65I AscI AseI AsnI
Asp718I BbrPI CpoI DraI FseI
HpaI KpnI KspI NotI PacI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SgrAI SnaBI SpeI
SwaI
###################################
# ONE CUT ENZYMES ( 19 enzymes) #
###################################
AflII 13566 CTTAAG C^TTAAG
AgeI 13687 ACCGGT A^CCGGT
BfrI 13566 CTTAAG C^TTAAG
BsiWI 9000 CGTACG C^GTACG
BspDI 11520 ATCGAT AT^CGAT
BssHII 3102 GCGCGC G^CGCGC
ClaI 11520 ATCGAT AT^CGAT
NdeI 12525 CATATG CA^TATG
NheI 13991 GCTAGC G^CTAGC
PaeR7I 10239 CTCGAG C^TCGAG
PinAI 13687 ACCGGT A^CCGGT
PshAI 1276 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 8637 GTCGAC G^TCGAC
SbfI 6870 CCTGCAGG CCTGCA^GG
ScaI 6763 AGTACT AGT^ACT
Sse8387I 6870 CCTGCAGG CCTGCA^GG
SspI 600 AATATT AAT^ATT
XbaI 10721 TCTAGA T^CTAGA
XhoI 10239 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AclI 984 AACGTT AA^CGTT
14361 AACGTT
Alw44I 3111 GTGCAC G^TGCAC
3201 GTGCAC
Aor51HI 9262 AGCGCT AGC^GCT
13045 AGCGCT
ApaLI 3111 GTGCAC G^TGCAC
3201 GTGCAC
AviII 3463 TGCGCA TGC^GCA
11863 TGCGCA
BsaBI 12824 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
13209 GATCCTCATC
BsmBI 11024 CGTCTC CGTCTC(1/5)
3417 GAGACG
EagI 26 CGGCCG C^GGCCG
11018 CGGCCG
EclXI 26 CGGCCG C^GGCCG
11018 CGGCCG
Eco47III 9262 AGCGCT AGC^GCT
13045 AGCGCT
Eco52I 26 CGGCCG C^GGCCG
11018 CGGCCG
EcoRI 4101 GAATTC G^AATTC
4962 GAATTC
EcoRV 9858 GATATC GAT^ATC
11010 GATATC
EcoT22I 2312 ATGCAT ATGCA^T
9288 ATGCAT
FspI 3463 TGCGCA TGC^GCA
11863 TGCGCA
MamI 12824 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
13209 GATCCTCATC
MluI 385 ACGCGT A^CGCGT
10100 ACGCGT
NsiI 2312 ATGCAT ATGCA^T
9288 ATGCAT
Psp1406I 984 AACGTT AA^CGTT
14361 AACGTT
#########################################
# 3 OR MORE CUT ENZYMES ( 194 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AhdI AluI
AlwI AlwNI AocI ApaI ApoI
Asp700I AspEI AspHI AspI AvaI
AvaII AvrII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcgI BciVI BclI BcnI BfaI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MroI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NruI NspI NspV PflFI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SfiI SfoI SfuI SmaI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmaCI XmaI XmnI