##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcgI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiWI
BsmBI BsoBI BspDI BspEI BspMI
BsrFI BssHII BssSI BstBI CelII
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccII 2283 CGCG CG^CG
Asp700I 1291 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 3335 GCTGAGG CCTCAGC(-5/-2)
BglII 3157 AGATCT A^GATCT
BsaWI 1053 TCCGGT [AT]^CCGG[AT]
BsgI 893 GTGCAG GTGCAG(16/14)
BsiEI 3981 CGATCG CG[GA][CT]^CG
Bsp1407I 3889 TGTACA T^GTACA
BspHI 2658 TCATGA T^CATGA
BspLU11I 3228 ACATGT A^CATGT
BsrGI 3889 TGTACA T^GTACA
BstAPI 2705 GCAAACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1423 GGTAACC G^GT[ACGT]ACC
BstPI 1423 GGTAACC G^GT[ACGT]ACC
BstUI 2283 CGCG CG^CG
CfoI 2284 GCGC GCG^C
DrdI 3477 GACTCTTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1492 CCGTGG C^C[GA][CT]GG
EcoO65I 1423 GGTAACC G^GT[ACGT]ACC
HapII 1054 CCGG C^CGG
HhaI 2284 GCGC GCG^C
HinP1I 2284 GCGC G^CGC
HpaI 2053 GTTAAC GTT^AAC
HpaII 1054 CCGG C^CGG
MspI 1054 CCGG C^CGG
MvnI 2283 CGCG CG^CG
NdeI 3488 CATATG CA^TATG
PflMI 2508 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuI 3981 CGATCG CGAT^CG
RcaI 2658 TCATGA T^CATGA
SspBI 3889 TGTACA T^GTACA
Van91I 2508 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 877 TCTAGA T^CTAGA
XcmI 1269 CCACATATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1291 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 2652 ACACGT A^C[GA][CT]GT
3228 ACATGT
AlwI 2579 GATCC GGATC(4/5)
3140 GATCC
AlwNI 95 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3144 CAGGAACTG
AseI 226 ATTAAT AT^TAAT
2011 ATTAAT
AsnI 226 ATTAAT AT^TAAT
2011 ATTAAT
AspI 1864 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2533 GACTCAGTC
BbsI 1820 GAAGAC GAAGAC(2/6)
3224 GAAGAC
BclI 1468 TGATCA T^GATCA
2721 TGATCA
BpuAI 1820 GAAGAC GAAGAC(2/6)
3224 GAAGAC
BsaI 103 GAGACC GGTCTC(1/5)
3116 GAGACC
BsrBI 1765 CCGCTC CCGCTC(-3/-3)
956 GAGCGG
BsrDI 336 CATTGC GCAATG(2/0)
1435 CATTGC
Bst1107I 3777 GTATAC GTA^TAC
4020 GTATAC
BstYI 2578 AGATCC [GA]^GATC[CT]
3157 AGATCT
BstZ17I 3777 GTATAC GTA^TAC
4020 GTATAC
EcoRV 449 GATATC GAT^ATC
3559 GATATC
EcoT14I 1144 CCTTGG C^C[AT][AT]GG
3318 CCAAGG
FbaI 1468 TGATCA T^GATCA
2721 TGATCA
HindIII 2564 AAGCTT A^AGCTT
3417 AAGCTT
MflI 2578 AGATCC [GA]^GATC[CT]
3157 AGATCT
PflFI 1864 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2533 GACTCAGTC
PshBI 226 ATTAAT AT^TAAT
2011 ATTAAT
SphI 112 GCATGC GCATG^C
3250 GCATGC
SspI 1948 AATATT AAT^ATT
2270 AATATT
StyI 1144 CCTTGG C^C[AT][AT]GG
3318 CCAAGG
Tth111I 1864 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2533 GACTCAGTC
XhoII 2578 AGATCC [GA]^GATC[CT]
3157 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AocI ApoI AspHI AvaII BalI
BbvI BciVI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
DraII EaeI EarI Eco81I EcoNI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I